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Metagenomic analysis of the community structure and functional potential of <i>Tamarix</i> rhizosphere microbiomes along a soil salinity gradient. [PDF]

Front Microbiol
Wang Y, Zhang L, Huang W, Wang N, Sun M, Wu L, Wang W, Shi C.   +7 more
europepmc   +1 more source

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Optimization of ectoine production from Nesterenkonia xinjiangensis and one-step ectoine purification

Bioresource Technology, 2023
In the current study, the optimization of ectoine production byNesterenkonia xinjiangensisand purification of ectoine from the bacterial cell extract were performed for the first time. Various carbon sources (glucose, sucrose, maltose, lactose, mannitol, and xylose) and nitrogen sources (ammonium nitrate, ammonium phosphate, ammonium chloride, ammonium
Furkan Orhan, Akın Akıncıoğlu
exaly   +5 more sources

Ectoine accumulation in Brevibacterium epidermis

Biotechnology Letters, 2004
As a halotolerant bacterial species, Brevibacterium epidermis DSM 20659 can grow at relatively high salinity, tolerating up to 2 M NaCl. It synthesizes ectoine and the intracellular content increases with the medium salinity, with a maximum of 0.14 g ectoine/g CDW at 1 M NaCl. Sugar-stressed cells do not synthesize ectoine.
Annelies, Onraedt, Cassandra, De Muynck, Bart, Walcarius, Wim, Soetaert, Erick, Vandamme   +4 more
openaire   +2 more sources

Efficient production of ectoine using ectoine-excreting strain

Extremophiles, 2009
Halophilic bacteria strain Halomonas salina DSM 5928 was found to excrete ectoine, suggesting its potential in the development of a new method of ectoine production. We performed HPLC and LC-MS analyses that showed that Halomonas salina DSM 5928 excreted ectoine under constant extracellular osmolarity.
Ling-hua, Zhang, Ya-jun, Lang, Shinichi, Nagata   +2 more
exaly   +3 more sources

Biotechnological production of ectoine: current status and prospects

Folia Microbiologica, 2023
Ectoine is an important natural secondary metabolite in halophilic microorganisms. It protects cells against environmental stressors, such as salinity, freezing, drying, and high temperatures. Ectoine is widely used in medical, cosmetic, and other industries.
Jun Chen, Deliang Qiao, Tao Yuan, Yeyuan Feng, Pengjun Zhang, Xuejun Wang, Li Zhang   +6 more
openaire   +2 more sources

Poly-β-hydroxybutyrate/ectoine co-production by ectoine-excreting strain Halomonas salina

Process Biochemistry, 2014
Abstract The ectoine-excreting bacterial strain of Halomonas salina was employed in the co-production of poly-β-hydroxybutyrate (PHB) and ectoine (Ect) during a fermentation process (PHB/Ect co-production). An efficient PHB/Ect co-production process was carried out at low NaCl concentration (30 g L−1).
Qing Chen, Linghua Zhang, Xiaolin Li, Sha Liu, Danni Li   +4 more
openaire   +1 more source

Ectoine hyperproduction by engineered Halomonas bluephagenesis

Metabolic Engineering
Ectoine, a crucial osmoprotectant for salt adaptation in halophiles, has gained growing interest in cosmetics and medical industries. However, its production remains challenged by stringent fermentation process in model microorganisms and low production level in its native producers.
Guo-Qiang Chen
exaly   +3 more sources

Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis

Metabolic Engineering, 2015
Advanced high-throughput screening methods for small molecules may have important applications in the metabolic engineering of the biosynthetic pathways of these molecules. Ectoine is an excellent osmoprotectant that has been widely used in cosmetics. In this study, the Escherichia coli regulatory protein AraC was engineered to recognize ectoine as its
Wei Chen, Shan Zhang, Peixia Jiang, Jun Yao, Yongzhi He, Lincai Chen, Xiwu Gui, Zhiyang Dong, Shuang-Yan Tang   +8 more
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Poly(ectoine) Hydrogels Resist Nonspecific Protein Adsorption

Langmuir, 2017
The development of nonfouling zwitterionic materials has a wide range of biomedical and engineering applications. This work delineates the design and synthesis of a new zwitterionic material based on a naturally occurring compatible solute, ectoine, which is known to possess additional protective properties that stabilize even whole cells against ...
Priyesh Jain, Hsiang-Chieh Hung, Xiaojie Lin, Jinrong Ma, Peng Zhang, Fang Sun, Kan Wu, Shaoyi Jiang   +7 more
openaire   +2 more sources

Supplementation effect of ectoine on thermostability of phytase

Journal of Bioscience and Bioengineering, 2006
In this study, we elucidated the supplementation effect of compatible solutes on the thermostability of phytase, designated as PHYA II, which was encoded by the phytase gene phyA I (GeneBank AY013315) from Aspergillus ficuum As3.324 and expressed in Pichia pastoris GS115.
Linghua, Zhang, Yue, Wang, Chunyu, Zhang, Yunji, Wang, Daochen, Zhu, Chenxiang, Wang, Shinichi, Nagata   +6 more
openaire   +2 more sources