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Hydration in aqueous solutions of ectoine and hydroxyectoine

Physical Chemistry Chemical Physics, 2018
We explore the influence of the two osmolytes ectoine and hydroxyectoine on the structure of pure water and aqueous NaCl solutions using non-resonant X-ray Raman scattering spectroscopy at the oxygen K-edge.
Christoph J. Sahle   +3 more
openaire   +3 more sources

Stabilization of bovine lactoperoxidase in the presence of ectoine

Food Chemistry, 2018
Lactoperoxidase (LPO) is a heme peroxidase with various applications in industry and medicine. In this study, the effects of ectoine, as a compatible solute, on the structure, thermal stability, thermodynamic parameters, activity, and stability of LPO have been investigated.
Hashem Nayeri
exaly   +3 more sources

Effect of ectoine on hydration spheres of peptides–spectroscopic studies

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2023
In this paper, we use FTIR spectroscopy to characterize the hydration water of ectoine, its interactions with two peptides-diglycine and NAGMA, and the properties of water molecules in the hydration spheres of both peptides changed by the presence of the osmolyte.
Aneta Panuszko   +5 more
openaire   +2 more sources

[Construction of ectoine absorption defective mutant for efficient ectoine production].

Wei sheng wu xue bao = Acta microbiologica Sinica, 2012
To further enhance ectoine (1, 4, 5, 6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) synthesis efficiency.We cloned Halomonas salina DSM 5928(T) teaABC gene of ectoine-specific transporter TeaABC by walking PCR and constructed ectoine absorption defective mutant H.
Rui, Xu, Linghua, Zhang
openaire   +1 more source

Microbial Production of Ectoine: A Review

ACS Synthetic Biology
Ectoine is an important natural secondary metabolite widely used in biomedical fields, novel cosmetics development, and the food industry. Due to the increasing market demand for ectoine, more cost-effective production methods are being explored. With the rapid development of synthetic biology and metabolic engineering technologies, the production of ...
Ke Wang, Boya Cui, Yi Wang, Wei Luo
openaire   +2 more sources

Hydration and Ion Binding of the Osmolyte Ectoine

The Journal of Physical Chemistry B, 2015
Ectoine is a widespread osmolyte enabling halophilic bacteria to withstand high osmotic stress that has many potential applications ranging from cosmetics to its use as a therapeutic agent. In this contribution, combining experiment and theory, the hydration and ion-binding of this zwitterionic compound was studied to gain information on the ...
Andreas, Eiberweiser   +4 more
openaire   +2 more sources

Ectoine interaction with DNA

2020
Ectoine is a small zwitterionic osmolyte and compatible solute, which does not interfere with cell metabolism even at molar concentrations. Plasmid DNA (pUC19) was irradiated with ultraviolet radiation (UV-C at 266 nm) under quasi physiological conditions (PBS) and in pure water in the presence and absence of ectoine (THP(B)) and hydroxyectoine (THP(A))
Hahn, Marc Benjamin   +4 more
openaire   +1 more source

Ectoines in cell stress protection: Uses and biotechnological production

Biotechnology Advances, 2010
Microorganisms produce and accumulate compatible solutes aiming at protecting themselves from environmental stresses. Among them, the wide spread in nature ectoines are receiving increasing attention by the scientific community because of their multiple applications.
José M, Pastor   +9 more
openaire   +2 more sources

Scalable Electro‐Biosynthesis of Ectoine from Greenhouse Gases

Angewandte Chemie International Edition
AbstractConverting greenhouse gases into valuable products has become a promising approach for achieving a carbon‐neutral economy and sustainable development. However, the conversion efficiency depends on the energy yield of the substrate. In this study, we developed an electro‐biocatalytic system by integrating electrochemical and microbial processes ...
Shuqi Guo   +11 more
openaire   +2 more sources

Accumulation of ectoine in the halotolerant Brevibacterium sp. JCM 6894

Journal of Bioscience and Bioengineering, 2001
The optimum conditions for the intracellular synthesis of the cyclic amino acid ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyridine carboxylic acid) were determined using the halotolerant Brevibacterium sp. JCM 6894. The amount of ectoine synthesized in cells was quantitatively determined after extraction from cells grown under various conditions, such as ...
S, Nagata, Y B, Wang
openaire   +2 more sources

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