Interactions between Upf1 and the decapping factors Edc3 and Pat1 in Saccharomyces cerevisiae. [PDF]
PLoS ONE, 2011 In Saccharomyces cerevisiae, mRNA transcripts with premature termination codons are targeted for deadenylation independent decapping and 5' to 3' decay in a quality control pathway termed nonsense-mediated decay (NMD).
Kylie D Swisher, Roy Parker
doaj +5 more sources
The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae [PDF]
Biology Open, 2016 The rate and regulation of mRNA decay are major elements in the proper control of gene expression. Edc3 and Lsm4 are two decapping activator proteins that have previously been shown to function in the assembly of RNA granules termed P bodies.
Susanne Huch +5 more
doaj +6 more sources
The structural basis of Edc3‐ and Scd6‐mediated activation of the Dcp1:Dcp2 mRNA decapping complex [PDF]
The EMBO Journal, 2011 The Dcp1:Dcp2 decapping complex catalyses the removal of the mRNA 5' cap structure. Activator proteins, including Edc3 (enhancer of decapping 3), modulate its activity. Here, we solved the structure of the yeast Edc3 LSm domain in complex with a short helical leucine-rich motif (HLM) from Dcp2.
Fromm, Simon A. +6 more
openaire +8 more sources
Identification of the Rps28 binding motif from yeast Edc3 involved in the autoregulatory feedback loop controlling RPS28B mRNA decay [PDF]
Nucleic Acids Research, 2013 In the yeast Saccharomyces cerevisiae, the Edc3 protein was previously reported to participate in the auto-regulatory feedback loop controlling the level of the RPS28B messenger RNA (mRNA). We show here that Edc3 binds directly and tightly to the globular core of Rps28 ribosomal protein.
Kolesnikova, Olga +3 more
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Global Phosphoproteomics Identifies a Major Role for AKT and 14-3-3 in Regulating EDC3
Molecular & Cellular Proteomics, 2010 Insulin plays an essential role in metabolic homeostasis in mammals, and many of the underlying biochemical pathways are regulated via the canonical phosphatidylinositol 3-kinase/AKT pathway. To identify novel metabolic actions of insulin, we conducted a quantitative proteomics analysis of insulin-regulated 14-3-3-binding proteins in muscle cells ...
Larance, Mark +6 more
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Transkriptionelle Charakterisierung des Kandidatengens EDC3 für mentale Retardierung [PDF]
, 2019 Background and aims: A homozygous variant in the Enhancer of Decapping 3 gene (EDC3, c.161T>C; p.Phe54Ser) was identified in a preliminary study of the research group in Erlangen in two siblings with mild, non-syndromic intellectual disability (ID). EDC3 interacts and enhances the decapping factor DCP2 which hydrolyses the 7-methylguanosine cap at the ...
Scheller, Ute
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Melatonin‐Mediated Intracellular Insulin during 2‐Deoxy‐
Oxidative Medicine and Cellular Longevity, 2016 2‐DG triggers glucose deprivation without altering other nutrients or metabolic pathways and then activates autophagy via activation of AMPK and endoplasmic reticulum (ER) stress. We investigated whether 2‐DG reduced intracellular insulin increased by melatonin via autophagy/EDC3 in insulinoma INS‐1E cells. p‐AMPK and GRP78/BiP level were significantly
Han Sung Kim +2 more
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, 2020 Processing bodies (P-bodies) are cytoplasmic mRNA granules that serve as hubs of mRNA repression, degradation, and processing. Initially described as membrane-less organelles, P-bodies stand at a junction between protein translation and RNA decay.
Bearss, Jeremiah
core +5 more sources
Med13 is required for efficient P-body recruitment and autophagic degradation of Edc3 following nitrogen starvation. [PDF]
Mol Biol CellThe Cdk8 kinase module (CKM), a conserved, detachable unit of the Mediator complex, plays a vital role in regulating transcription and communicating stress signals from the nucleus to other organelles. Here, we describe a new transcription-independent role for Med13, a CKM scaffold protein, following nitrogen starvation.
Hanley SE +3 more
europepmc +3 more sources
Identification and Analysis of the Interaction between Edc3 and Dcp2 in Saccharomyces cerevisiae [PDF]
Molecular and Cellular Biology, 2010 Cap hydrolysis is a critical control point in the life of eukaryotic mRNAs and is catalyzed by the evolutionarily conserved Dcp1-Dcp2 complex. In Saccharomyces cerevisiae, decapping is modulated by several factors, including the Lsm family protein Edc3, which directly binds to Dcp2.
Yuriko, Harigaya +4 more
openaire +2 more sources