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Cryo-Electron Tomography and Subtomogram Averaging

Methods in Enzymology, 2016
Cryo-electron tomography (cryo-ET) allows 3D volumes to be reconstructed from a set of 2D projection images of a tilted biological sample. It allows densities to be resolved in 3D that would otherwise overlap in 2D projection images.
Briggs, J., W. Wan, Wan, W., J.A.G. Briggs   +3 more
exaly   +2 more sources

Integrative Approaches for Cellular Cryo-Electron Tomography: Correlative Imaging and Focused Ion Beam Micromachining

Methods in Cell Biology, 2012
The application of cryo-electron tomography to cells and tissues is commonly referred to as “cellular tomography,” and enables visualization of the supramolecular architecture of cells in a near-native state.
Benjamin D Engel, Alexander Rigort, Juergen M. Plitzko   +2 more
exaly   +2 more sources

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Analysis of Mitochondrial Membrane Protein Complexes by Electron Cryo-tomography

2017
The visualization of membrane protein complexes in their natural membrane environment is a major goal in an emerging area of research termed structural cell biology. Such approaches provide important information on the spatial distribution of protein complexes in their resident cellular membrane systems and on the structural organization of multi ...
Gold, Vicki A. M., Brandt, Tobias, Cavellini, Laetitia, Cohen, Mickael M., Ieva, Raffaele, Laan, Martin van der   +5 more
openaire   +4 more sources

High-Throughput Electron Cryo-tomography of Protein Complexes and Their Assembly

2018
Electron cryo-tomography and subtomogram averaging enable visualization of protein complexes in situ, in three dimensions, in a near-native frozen-hydrated state to nanometer resolutions. To achieve this, intact cells are vitrified and imaged over a range of tilts within an electron microscope.
Louie D, Henderson, Morgan, Beeby
openaire   +2 more sources

Preparation of Bunyavirus-Infected Cells for Electron Cryo-Tomography

Cellular electron cryo-tomography (cryoET) produces high-resolution three-dimensional images of subcellular structures in a near-native frozen-hydrated state. These three-dimensional images are obtained by recording a series of two-dimensional tilt images on a transmission electron cryo-microscope that are subsequently back-projected to form a tomogram.
Fanny Ott, Märit-Runa Jönsson, Kay Grünewald, Jan Hellert   +3 more
openaire   +3 more sources

Electron Cryo-Tomography of Viral Infection Within Caulobacter crescentus

Microscopy and Microanalysis, 2008
Extended abstract of a paper presented at Microscopy and Microanalysis 2008 in Albuquerque, New Mexico, USA, August 3 – August 7 ...
ER Wright, JS Poindexter, P Viollier, GJ Jensen   +3 more
openaire   +1 more source

In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography

2020
Electron cryo-tomography (cryo-ET) is a technique that allows the investigation of intact macromolecular complexes while they are in their cellular milieu. Over the years, cryo-ET has had a huge impact on our understanding of how large biomolecular complexes look like, how they assemble, disassemble, function, and evolve(d).
Kaplan, Mohammed, Nicolas, William J., Zhao, Wei, Carter, Stephen D., Metskas, Lauren Ann, Chreifi, Georges, Ghosal, Debnath, Jensen, Grant J.   +7 more
openaire   +3 more sources

Cryo-Electron Tomography: The Resolution Revolution and a Surge of in Situ Virological Discoveries

Annual Review of Biophysics, 2023
Yutong Song, Sai Li
exaly  

Unveiling the Polarity of Actin Filaments by Cryo-Electron Tomography

Structure, 2021
Bruno Martins, Simona Sorrentino, Matthias Eibauer   +2 more
exaly  

Electron Cryo-Microscopy and Cryo-Tomography of Viruses☆

2015
W. Chiu, C. Hecksel, C.F. Hryc
openaire   +1 more source