Results 161 to 170 of about 1,487,249 (257)

A Rapid Method to Rank Proteins of Widely Varying Stability: Application to Paramyxovirus Foot Domains. [PDF]

Curr Protoc
Hausrath AC, Kingston RL, McEvoy MM.
europepmc   +1 more source

Programmable Assembly of Higher‐Order DNA Nanostructures from Microbial ssDNA Staple Libraries

Small, EarlyView.
A phage‐free, microbial platform produces programmable circular ssDNA libraries that are enzymatically converted into DNA origami staple pools. Rolling‐circle replication generates sequence‐complex ssDNA with >99% fidelity, enabling assembly of DNA tiles and tubules and supporting nanostructure formation even in crude cellular lysates.
Ming Hung Yen, Mallikarjuna Reddy Kesama, Yancheng Du, Jong Hyun Choi, Kevin V. Solomon   +4 more
wiley   +1 more source

Prevalence, Antimicrobial Susceptibility, and Hygienic Practices of Escherichia coli O157:H7 in Raw Meat, Bahir Dar City, Ethiopia. [PDF]

Vet Med Sci
Asefa W, Tassew H, Bialfew T, Gelaye E, Azene BT.   +4 more
europepmc   +1 more source

Exploiting Phase Partitioning of a Giant Trimeric Fibrous Protein Scaffold to Potentiate Chitinase‐Driven Antifungal Activity

Small, EarlyView.
Cstalk, a giant fibrous protein, selectively partitions into the dextran‐rich phase in polyethylene glycol/dextran aqueous two‐phase systems, functioning as a recruiter for proteins of interest. This phase‐selective recruitment can be extended to fungal cell walls, enabling the targeted localization of enzymes and enhancing antifungal activity through ...
Ayasa Nagatani, Shogo Yoshimoto, Toki Taira, Rie Wakabayashi, Masahiro Goto, Katsutoshi Hori, Noriho Kamiya   +6 more
wiley   +1 more source

CryoBN‐PAGE: A Rapid and Cost‐Effective Method for the Analysis of Yeast Respiratory Complexes and Their Dynamic Organization

Yeast, EarlyView.
We present cryoBN‐PAGE, a method that enables blue native polyacrylamide gel electrophoresis analysis of mitochondrial respiratory complexes and supercomplexes directly from whole yeast cells using cryogenic milling. This approach eliminates the need for mitochondrial isolation, reducing time and cost while preserving native protein complexes.
Lizeth J. Camacho Lopez, Mazzen H. Eldeeb, Elliana G. Mohrhardt, Flavia Fontanesi   +3 more
wiley   +1 more source

Plasmid Curing of <i>Pseudoalteromonas haloplanktis</i> TAC125 Using Homologous Recombination and PTasRNA Gene Silencing. [PDF]

Bio Protoc
Severino A, Lauro C, Calvanese M, Parrilli E, Tutino ML.   +4 more
europepmc   +1 more source

Uneven Culturable Yeast Communities Harbored by Two Sympatric Stingless Bee Species: Scaptotrigona depilis and Tetragona clavipes

Yeast, EarlyView.
To explore the yeast diversity associated with Brazilian stingless bees, we collected samples from bees and their provisions. Sympatric bee species harbor distinct yeast communities, providing insights into bees' foraging choices. Moreover, these communities are dominated by specific taxa with similar metabolic profiles.
Rodolfo Bizarria Jr., Ana Ligia Leandrini de Oliveira, Bárbara Gimenes de Castro, Mônica Tallarico Pupo   +3 more
wiley   +1 more source

Draft genome sequences of four bacterial isolates from the Indian Fort Nature Preserve in Geneseo, NY. [PDF]

Microbiol Resour Announc
Cappa J, Privitera G, Rivera A, Hutchison E.   +3 more
europepmc   +1 more source

Taxogenomic Characterization and Comparative Genomic Analysis of Hermanozyma buritii sp. nov., f.a., a Yeast Species Isolated From Plants and Soil

Yeast, EarlyView.
Isolation of the novel yeast species Hernanozyma buritii sp. nov. from soil and plant in Brazil, followed by phylogenetic characterization and genome analysis, highlighting its relationship with closely related taxa and genomic features associated with mating, pulcherriminic acid biosynthesis, and iron‐dependent pigmentation.
Vitória Petra de O. Barros, Ana Raquel O. Santos, Júlio Ricardo M. Silva, Paula B. Morais, Thiago M. Batista, Daniela L. Souza, Rosangela Santa‐Brígida, Marlúcia B. Martins, Glória R. Franco, Marc‐André Lachance, Carlos A. Rosa, Melissa Fontes Landell   +11 more
wiley   +1 more source

Nutrimental Determinants of Chronological Aging and Competitiveness in the snf1Δ Warburg Model

Yeast, EarlyView.
Deletion of SNF1 in Saccharomyces cerevisiae alters chronological aging in a nutrient‐dependent manner, shortening lifespan under low‐nutrient conditions and extending it under high‐glucose and high‐amino‐acid conditions. Under nutrient‐rich conditions, the snf1∆ strain also showed an approximately 2‐log increase in competitiveness relative to the wild
Abigail Correa‐Olivares, Amanda de la Caridad Lahera Champagne, Alma Delia Bertadillo‐Jilote, Karla I. Lira‐de Leon, David G. Garcia‐Gutierrez, Gerardo M. Nava, Vanessa Sanchez‐Quezada, Luis Alberto Madrigal‐Perez   +7 more
wiley   +1 more source