Results 171 to 180 of about 1,417,832 (307)

Blocking protein quality control degradation leads to structural stabilization of DHFR indel variants

The FEBS Journal, EarlyView.
Blocking protein quality control degradation by deleting the E3 ligases Ubr1 or San1 stabilizes many DHFR insertion and deletion variants that are otherwise partially unfolded. These hypomorphic variants regain folding at lower temperatures or upon methotrexate binding, revealing that PQC degradation can remove proteins that are still functionally ...
Sven Larsen‐Ledet, Caroline H. Suhr, Sarah Gersing, Celeste M. Hackney, Amelie Stein, Kaare Teilum, Rasmus Hartmann‐Petersen   +6 more
wiley   +1 more source

Synergistic anti-Helicobacter pylori efficacy of a molecularly identified Limosilactobacillus fermentum isolate in combination with multiple antibiotics. [PDF]

BMC Microbiol
Ali MMMM, Shady HMA, M SM, Sayed HAE.
europepmc   +1 more source

Functional and transcriptomic analyses in Neurospora crassa reveal a crucial role for the N‐glycoprotein deglycosylation process in fungal homeostasis

The FEBS Journal, EarlyView.
In Neurospora crassa, a cytosolic GH18 endo‐β‐n‐acetylglucosaminidase (ENGase) functions as an active deglycosylating enzyme. Deletion of this severely alters tolerance to multiple stress conditions and causes extensive transcriptomic reprogramming, including upregulation of a GH20 exo‐β‐n‐acetylhexosaminidase under ER stress, indicating that N. crassa
Anastasios Samaras, Tanim Jabid Hossain, Magnus Karlsson, Georgios Tzelepis   +3 more
wiley   +1 more source

Cuantificación de hemoglobina A2 por electroforesis en gel de agarosa Quantitation of hemoglobin A2 by electrophoresis in agar gel

Revista Cubana de Hematología, Inmunología y Hemoterapia, 2012
Maydelín Miguel-Morales, Mabel Díaz-Barroso, Heidys Garrote-Santana, Grisel Uley-del Rosario, Graciela Pérez-Diez de los Ríos, Marianela Estrada-del Cueto   +5 more
doaj  

Affordable CRISPR RNP-Based Genome Editing in Euglena gracilis. [PDF]

Curr Protoc
Minami A, Shimizu M, Tamaki S, Nishinarizki V, Yosua, Mochida K.   +5 more
europepmc   +1 more source

Insights into tandem dioxygenases—copper radical oxidases in fungal Phytopathogens of the Colletotrichum acutatum species complex

The FEBS Journal, EarlyView.
In Colletotrichum acutatum species complex, conserved ring‐cleavage dioxygenases located adjacent to AA5_2 enzymes were investigated as potential redox partners. Biochemical analyses identified the dioxygenases as intradiol‐cleaving enzymes preferentially oxidizing methyl‐substituted catechols, while AA5_2 enzymes displayed weak alcohol oxidase ...
Radka Končitíková, David Ribeaucourt, Mireille Haon, David Navarro, Elodie Drula, Bastien Bissaro, Jean‐Guy Berrin, Mickael Lafond   +7 more
wiley   +1 more source

Protocol for ex vivo mouse paired single-gonad screening platform for phenotypical characterization. [PDF]

STAR Protoc
Estermann MA, Rodriguez KF, Hunnicutt J, Yao HH.   +3 more
europepmc   +1 more source

Outbreaks of Granulomatous Disease Associated With Vibrio harveyi Infection in Grouper (Epinephelus fuscoguttatus × E. lanceolatus) Aquaculture in Taiwan

Journal of Fish Diseases, EarlyView.
ABSTRACT Between 2015 and 2024, outbreaks of muscular and visceral granulomatous disease, resulting in moderate‐to‐severe mortality in farmed groupers, occurred in southern Taiwan. Initially, affected groupers were screened for granuloma‐associated pathogens, including Mycobacterium spp., Nocardia spp., Photobacterium damselae and Edwardsiella spp ...
Shang‐Ting Wu, Chia‐Yu Hsieh
wiley   +1 more source

Sensitivity of Loop-Mediated Isothermal Amplification in Comparison to Digital Droplet PCR for Identification of <i>Yersinia pseudotuberculosis</i> in Raw Goat Milk. [PDF]

Foods
Kim TC, Zaharieva MM, Najdenski HM.
europepmc   +1 more source

Enrichment‐Based Duplex PCR for Early Detection of Potentially Virulent Vibrio harveyi in Aquaculture

Journal of Fish Diseases, EarlyView.
ABSTRACT Vibrio harveyi is an important bacterial pathogen in marine aquaculture, responsible for outbreaks affecting a wide range of fish species. Early detection of potentially virulent isolates in fish and environmental samples is essential for effective disease prevention and management, particularly under field conditions where the pathogen may be
Javier Barriga‐Cuartero, Carla Hernández‐Cabanyero, Pablo Ibanyez‐Paya, Carmen Amaro, Belen Fouz   +4 more
wiley   +1 more source