Results 91 to 100 of about 99,285 (246)

Structurally Enhanced Colloidal Molecules via Carbene‐Mediated Covalent C─H Insertion Crosslinking

Advanced Science, EarlyView.
This work presents a nonspecific covalent crosslinking strategy to significantly enhance the structural stability of colloidal molecules (CMs) without compromising their inherent structural and physicochemical properties by rationally designing a diazo‐based crosslinker.
Liwei Dai, Chongyang Yao, Xiancheng Zhang, Weijia Kong, Mingxing Liu, Huibin He, Yutao Sang, Zhihong Nie   +7 more
wiley   +1 more source

Modular Assembly of Dynamic Polymer Networks From Heteroaffinity Cross‐Links to Multivalent Proteins

Angewandte Chemie, EarlyView.
This study develops a method to modularly assemble oligomeric binding proteins, like streptavidin, using heterobifunctional cross‐linkers that have both a strong‐ and a weak‐affinity binding moiety. These dynamic cross‐linkers enable the assembly of polymer networks with unique meta‐stable nonequilibrium topologies, and that exhibit chemically tunable ...
Tianyue Dai, Yuntao Qiu, Katherine M. Leon Hernandez, Wesley Chan, Katrina Mejia, Natalia Nemeria, Colin D. Kinz‐Thompson   +6 more
wiley   +2 more sources

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

Advanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

Visualizing and Quantifying microRNA‐Induced DNA Origami Separation at the Nanoscale

Angewandte Chemie, EarlyView.
Clinically relevant miRNA biomarkers trigger the disassembly of DNA origami dimers into monomers through a toehold‐mediated strand displacement reaction. High‐speed AFM was used to visualize this reaction in real time, while solid‐state nanopore measurements quantified the populations of dimers and monomers, as well as the resulting miRNA concentration,
Chalmers C. C. Chau, Varun Gupta, George R. Heath, Christoph Wälti, Paolo Actis   +4 more
wiley   +2 more sources

Enhanced Intracellular Stability and Translation Efficiency of mRNA Drugs by a 2‐arm mRNA Platform

Advanced Science, EarlyView.
We constructed a 2‐arm mRNA, characterized by a unique topology formed through the dimerization of two mRNA 3’ tails. The 2‐arm mRNA improves 3’ tail stability and resistance to nuclease degradation, resulting in an intracellular half‐life of up to 65 h. This method substantially enhances the translation capacity of mRNA drugs.
Xucong Teng, Jiahao Lin, Qiushuang Zhang, Xiangdong Zhang, Yicong Dai, Jinghong Li   +5 more
wiley   +1 more source

Inverse Shifting-PCR Modified by Capillary Electrophoresis for Detecting F8 int22h and int1h Inversions in Severe Hemophilia A Patients and Probable Carriers

Life
Globally, intron 22 inversions (Inv22s) of the factor VIII gene (F8) are the most frequent pathogenic variants and account for 45–50% of severe hemophilia A (SHA) cases, while intron 1 inversion (Inv1) explains 1–5% of SHA cases.
Rosa Michel Martínez-Contreras, Silvia Sofía García-López, Hilda Luna-Záizar, Ana Rebeca Jaloma-Cruz   +3 more
doaj   +1 more source

Selective Electrolysis of Water Under Artificial Seawater Conditions Using Transition Metal Borate Anodes

Advanced Energy Materials, EarlyView.
The borate‐based cobalt‐iron catalyst promotes selective oxidation of water rather than chlorine evolution reaction during electrolysis of seawater. When integrated into a zero‐gap electrolyzer prototype, this catalyst supports robust splitting of artificial seawater into oxygen and hydrogen on an extended timescale.
Ananta R. Fareza, Hank Garg, Luis Miguel Azofra, Darcy Simondson, Tam D. Nguyen, Mohamed R. Rizk, Khang N. Dinh, Rosalie K. Hocking, Douglas R. MacFarlane, Hoang‐Long Du, Alexandr N. Simonov   +10 more
wiley   +1 more source

Artifacts of analysis in cell line identification by short tandem repeat profiling

Кардиоваскулярная терапия и профилактика
Aim. To study and describe the most common types of artifacts in detection of short tandem repeat (STR) amplicons by capillary electrophoresis and cause difficulties in interpreting the obtained STR profiles.Material and methods. Cell lines were obtained
A. A. Malchenkova, E. N. Kosobokova
doaj   +1 more source

Novel MYL1 Intron Variant With Expanded Phenotype

American Journal of Medical Genetics Part A, EarlyView.
ABSTRACT Congenital myopathy‐14 (CMYO14) is an ultrarare autosomal recessive disorder caused by biallelic variants in MYL1, with only four patients reported to date. We describe what is likely the fifth reported patient, a neonate with severe hypotonia, respiratory insufficiency, and skeletal anomalies showing distinct histological changes of skeletal ...
Maria Barington, Marie Balslev‐Harder, Thomas Krag, Thomas van Overeem Hansen, Camilla Bernt Wulff, Ulrik Lausten‐Thomsen, Tina Duelund Hjortshøj, Elsebet Østergaard   +7 more
wiley   +1 more source

Carbon nanotubes in capillary electrophoresis, capillary electrochromatography and microchip electrophoresis

Open Chemistry, 2012
Pauwels Jochen, Schepdael Ann
doaj   +1 more source