Results 131 to 140 of about 205,159 (276)

Endothelial PD‐1 Regulates Vascular Homeostasis and Oligodendrogenesis during Brain Development

Advanced Science, EarlyView.
Vascular endothelial cells are fundamental for angiogenesis and brain development. This study reveals that endothelial programmed cell death 1 (PD‐1) is critical for cerebral angiogenesis and blood brain barrier (BBB) maturation. It also mediates interaction with oligodendrocyte precursor cells (OPCs), modulating OPCs differentiation and myelination ...
Tingting He, Mengtian Zhang, Jie Qin, Yanyan Wang, Sihan Li, Chaoyi Du, Jianwei Jiao, Fen Ji   +7 more
wiley   +1 more source

Switching positions: Assessing the dynamics of conjugational heterogeneity in antibody–drug conjugates using CE‐SDS

ELECTROPHORESIS, Volume 44, Issue 1-2, Page 62-71, January 2023., 2023
Abstract Antibody–drug conjugates (ADCs) are a prospective class of new oncology therapeutics with the ability to deliver a cytotoxic drug to a targeted location. The concept appears simple, but ADCs are highly complex due to their intrinsic heterogeneity. Randomly conjugated ADCs, for instance, are composed of conjugated species carrying between 0 and
Eline B. A. van den Berg, Jaap C. W. Hendriks, Everdine W. Elsinga, Mark Eggink, Eef H. C. Dirksen   +4 more
wiley   +1 more source

Outermost Cationic Surface Charge of Layer‐by‐Layer Films Prevents Endothelial Cells Migration for Cell Compartmentalization in Three‐Dimensional Tissues

Advanced Science, EarlyView.
A nanometer‐sized artificial basement membrane (A‐BM) is developed to replicate the barrier functions of the basement membrane and prevent cell migration. It is demonstrated that the outermost cationic surface charge of nanofilms can effectively inhibit endothelial cell migration and sprouting, allowing for the construction of 3D patterned vascular ...
Jinfeng Zeng, Sven Heilig, Matthias Ryma, Jürgen Groll, Congju Li, Michiya Matsusaki   +5 more
wiley   +1 more source

Method development for quantitative monitoring of monoclonal antibodies in upstream cell‐culture process samples with limited sample preparation – An evaluation of various capillary coatings

ELECTROPHORESIS, Volume 44, Issue 1-2, Page 96-106, January 2023., 2023
Abstract Monoclonal antibodies (mAbs) have become an important class of biopharmaceuticals used for the treatment of various diseases. Their quantification during the manufacturing process is important. In this work, a capillary zone electrophoresis (CZE) method was developed for the monitoring of the mAb concentration during cell‐culture processes ...
Debbie van der Burg, Hermann Wätzig, Cari E. Sänger‐van de Griend   +2 more
wiley   +1 more source

A Fast Stable Discretization of the Constant-Convection-Diffusion-Reaction Equations of Kinetic Capillary Electrophoresis (KCE) [PDF]

arXiv, 2016
A discretization scheme is introduced for a set of convection-diffusion equations with a non-linear reaction term, where the convection velocity is constant for each reactant. This constancy allows a transformation to new spatial variables, which ensures the global stability of discretization. Convection-diffusion equations are notorious for their lack
arxiv  

Locoregional Immune Checkpoint Blockade and Remodeling of Lymph Nodes by Engineered Dendritic Cell‐Derived Exosomes for Suppressing Tumor Progression and Metastasis

Advanced Science, EarlyView.
The study develops engineered exosomes (EmDEX@GA) for locoregional immunomodulation of tumor‐draining lymph node (TDLN). EmDEX@GA can actively target to TDLN and remodel its immunosuppressive microenvironment through the synergistic effects of PD‐1/PD‐L1 blockade and STING pathway activation, subsequently enhance anti‐tumor immunity and suppress tumor ...
Yizhen Wang, Xiaomin Guo, Jingya Qin, Yifan Xue, Peng Zhang, Yadong Liu, Moyang Chen, Guanghao Zhu, Xinqiu Song, Lili Cheng, Bo Liu, Jie Liu, Jie Ren   +12 more
wiley   +1 more source

Enantioselective CE–MS analysis of ketamine metabolites in urine

ELECTROPHORESIS, Volume 44, Issue 1-2, Page 125-134, January 2023., 2023
Abstract The chiral drug ketamine has long‐lasting antidepressant effects with a fast onset and is also suitable to treat patients with therapy‐resistant depression. The metabolite hydroxynorketamine (HNK) plays an important role in the antidepressant mechanism of action.
Friederike A. Sandbaumhüter, Jordan T. Aerts, Regula Theurillat, Per E. Andrén, Wolfgang Thormann, Erik T. Jansson   +5 more
wiley   +1 more source

Estimating Kinetic Rate Constants and Plug Concentration Profiles from Simulated KCE Electropherogram Signals [PDF]

arXiv, 2017
Kinetic rate constants fundamentally characterize the dynamics of the chemical interaction of macromolecules, and thus their study sets a major direction in experimental biochemistry. The estimation of such constants is often challenging, partly due to the noisiness of data, and partly due to the theoretical framework.
arxiv  

Engineering Extracellular Vesicles Derived from 3D Cultivation of BMSCs Enriched with HGF Ameliorate Sepsis‐Induced Lung Epithelial Barrier Damage

Advanced Science, EarlyView.
3D culture of MSCs upregulates gene expression of matrix metalloproteinases (MMPs), growth factors (GFs), and increases the production of extracellular vesicles (EVs). Proteomic analysis reveals 642 upregulated proteins in 3D‐EVs, including HGF. These 3D‐EVs, enriched with HGF, protect lung epithelial cells from apoptosis and barrier damage in septic ...
Yong Chen, Feihong Lin, Tong Zhang, Zhuoran Xiao, Yuanli Chen, Dongsheng Hua, Yu Wang, Juan Wei, Tian Jin, Xin Lv   +9 more
wiley   +1 more source

Analysis of drug interactions with serum proteins and related binding agents by affinity capillary electrophoresis: A review

ELECTROPHORESIS, Volume 43, Issue 23-24, Page 2302-2323, December 2022., 2022
Abstract Biomolecules such as serum proteins can interact with drugs in the body and influence their pharmaceutical effects. Specific and precise methods that analyze these interactions are critical for drug development or monitoring and for diagnostic purposes.
Sadia Sharmeen, Isaac Kyei, Arden Hatch, David S. Hage   +3 more
wiley   +1 more source