Results 141 to 150 of about 226,631 (305)

High‐Resolution Multiplexed Sequencing of Single‐Cell Full‐length Transcriptome Via Combinational Barcoded Tn5 Transposon Insertion

Advanced Science, EarlyView.
CBTi‐seq is reported, leveraging Tn5 transposase‐mediated molecular assembly of combinatorial barcodes and UMIs for high‐resolution multiplexed sequencing of the full‐length transcriptome in single cells. This approach achieves molecular resolution by end‐to‐end sequencing, enabling reconstruction of splice variants and accurate quantification of ...
Liyong He, Kaitong Dang, Qian Sun, Wenjia Wang, Wenbo Li, Wenyi Zhang, Kaiqiang Ye, Handong Wang, Zhengyue Li, Yan Guo, Zheng Li, Chencheng Yao, Peng Li, Yan Huang, Xiangwei Zhao   +14 more
wiley   +1 more source

Pirin Transcriptionally Regulates PLA2G4A To Inhibit Ferroptosis in Colorectal Cancer via Lipid Profile Remodeling

Advanced Science, EarlyView.
This study identifies Pirin (PIR), an iron‐binding protein, as a critical ferroptosis suppressor in colorectal cancer through lipid membrane remodeling. PIR, induced by NRF2 during ferroptotic stress, transcriptionally regulates PLA2G4A to shift cellular lipid composition away from ferroptosis‐permissive polyunsaturated phospholipids.
Wei Shi, Yue Qi Ong, Prativa Majee, Esther SM Wong, Amhed Missael Vargas Velazquez, Kerem Fidan, Pierce Kah‐Hoe Chow, Wai Leong Tam, Ker Kan Tan, Iain Beehuat Tan, Vinay Tergaonkar   +10 more
wiley   +1 more source

Aptamer Engineering: Strategies for Discovering Functional Nucleic Acids for Next‐Generation Diagnostics and Biosensing

Advanced Science, EarlyView.
The advent of aptamers has highlighted their potential as alternatives to antibodies, overcoming limitations of structural instability and production cost. However, conventional approaches such as SELEX remain slow and labor‐intensive. This review examines recent advances in aptamer engineering, emphasizing in vitro and AI‐driven in silico strategies ...
John V. L. Nguyen, Ahlem Meziadi, Cyrill Brunner, Maureen McKeague, Lidija Malic, Maryam Tabrizian   +5 more
wiley   +1 more source

Multifunctional Nano Immunostimulant: Overcoming Immunosuppressive Microenvironment for Antitumor Immunotherapy

Advanced Science, EarlyView.
A multifunctional nano immunostimulant is first proposed for efficient tumor immunotherapy. The ER targeted ability, superior ROS production, efficient GSH consumption, coupled with the HIF suppression, jointly arouses ICD/ferroptosis pathways to realize efficient tumor immunotherapy.
Guanhong Guo, Wenda Zhong, Huishuang Zhao, Yueying An, Xinyu Dong, Zhengbo Li, Shuangfeng Qin, Guangzhao Xu, Xiangguo Yue, Xudong Wang, Wen Sun, Zhe‐Sheng Chen, Weiguo Song, Liuya Wei, Fahui Li   +14 more
wiley   +1 more source

Development of Dimethylsulfonium Probes for Broad Profiling of Methyllysine Reader Proteins

Advanced Science, EarlyView.
Development of oligoglycine‐based dimethylsulfonium probes for unbiased crosslinking to methyllysine readers. The general probe facilitates profiling of site‐specific methyllysine readers, evaluation of selectivity and activity of reader inhibitors, and global profiling of methyllysine readers.
Jinyu Yang, Yihang Xiao, Yingxiao Gao, Mingxuan Wu   +3 more
wiley   +1 more source

Enhanced Recognition via Defect Engineered Amorphous Metal–Organic Framework for Sensitive and Stable Pesticide Biosensor

Advanced Science, EarlyView.
A defect‐engineered assembly strategy is reported for the in situ encapsulation of acetylcholinesterase (AChE) into amorphous MOF‐74, endowing the composite with superior catalytic activity and target recognition capability. Leveraging these structural advantages, a sensitive and stable biosensor is constructed for the detection of paraoxon pesticide ...
Changshun Su, Xiangyu Zhai, Meng Zhang, Mengxue Li, Xinru Zhang, Chunyan Sun, Xu Yan, Hongxia Li   +7 more
wiley   +1 more source

GSH as an A‐A Type Allosteric Activator of PKM2: Modulating Cancer Cell Homeostasis and Ferroptosis Susceptibility

Advanced Science, EarlyView.
Glutathione (GSH) defines oxidative balance, but can it directly tune central metabolism? The research reveals that GSH binds and potently activates pyruvate kniase M2 (PKM2), rewiring glycolysis and dictating ferroptosis sensitivity. Combining GSH depletion with PKM2 activation triggers mitochondrial damage and lipid peroxidation, profoundly ...
Tsan‐Jan Chen, Chi‐Jen Lo, Meng‐Jen Wu, Wei Yang Sit, Hsin‐Yu Hsu, Yi‐Cheng Huang, Chien‐Hung Lu, Yu‐Lun Chen, Wei‐Kai Fang, Shan‐Min Yang, Pei‐Lien Chen, Tokuko Haraguchi, Yasushi Hiraoka, Chun‐Yu Lin, Mei‐Ling Cheng, Muh‐Hwa Yang, Hsing‐Jien Kung, Wen‐Ching Wang   +17 more
wiley   +1 more source

RADA16 and SAAP148 Peptide‐Modified Collagen Self‐Assembled Hydrogels for Accelerated Healing of Infected Wounds

Advanced Science, EarlyView.
Collagen type I (Col I) is integrated with self‐assembling peptide RADA16 and loaded with anti‐bacterial peptide SAAP148 to develop a self‐assembled nano‐microstructure hydrogel (Col I‐RADA16‐SAAP148, CRS) without chemical cross‐linkers. CRS exhibits excellent mechanical properties, sustained SAAP148 release to inhibit bacterial infection, macrophage ...
Qian Liu, Jiawei Wu, Ho‐Pan Bei, Yufei Chen, Yifan Zhang, Xueliang Peng, Fulin Chen, Xin Zhao, Zhuoyue Chen   +8 more
wiley   +1 more source

Diazirines Beyond Photoaffinity Labeling: A Comprehensive Overview of Applications in Biological Sciences, Materials Chemistry, and NMR‐Spectroscopy

Angewandte Chemie, EarlyView.
Diazirines are three‐membered, nitrogen‐containing heterocycles that decompose under light or heat to generate carbenes. For the past two decades, they have served mainly as minimally invasive tags in photoaffinity labeling (PAL). More recently, their value as versatile carbene precursors has been recognized.
Dominik Schnalzer, Viktor Savic, Clemens Cziegler, Michael Schnürch, Marko D. Mihovilovic   +4 more
wiley   +2 more sources

A Sirtuin‐1‐Targeted Gene‐Activating Tetrahedral DNA Attenuates Bladder Fibrosis by Restoring Mitophagy in Fibroblasts via the SIRT1‐FOXO3‐BNIP3 Axis

Advanced Science, EarlyView.
The SIRT1‐targeted saRNA‐delivering tetrahedral DNA (TSA) treatment effectively upregulates SIRT1 expression, which subsequently promotes FOXO3A deacetylation. This deacetylation event relieves FOXO3A's transcriptional repression on the BNIP3 gene, thereby initiating PINK1‐PARKIN‐dependent mitophagy.
Wei Wang, Ran Yan, Lede Lin, Lei Xiang, Xiaozhi Xia, Liao Peng, Xiaoshuai Gao, Jiawei Chen, Yang Xiong, Liang Zhou, Yunfeng Lin   +10 more
wiley   +1 more source