Results 51 to 60 of about 222,058 (260)

Highly Sensitive Detection of Human Serum Albumin Biomarker Using a Molecularly Imprinted Polymer‐Based Electrochemical Capacitance Sensor

Advanced Sensor Research, EarlyView.
An electrosynthesized protein‐imprinted polymer (poly(azo‐BBY)) enables probe‐free, real‐capacitance detection of human serum albumin at 0.02 ng mL−1 within a 0.25–3.0 ng mL−1 window. The device uses a redox‐mediator‐free architecture and maintains selectivity against common interferents, illustrating a simple, scalable route to high‐sensitivity ...
Heitor F. Trevizan, Pedro C. M. Pizzol, André Olean‐Oliveira, Patrícia Monteiro Seraphim, Marcos F. S. Teixeira   +4 more
wiley   +1 more source

Microchip Capillary Electrophoresis [PDF]

, 2014
For the study of neuromodulation in Cancer borealis we have designed a microfluidic device to separate and detect bioamine concentrations with a high temporal resolution.
Hardy, Scott, Schull, Daniel, Vinopal, Mark   +2 more
core   +1 more source

Microfluidics: Fluid physics at the nanoliter scale [PDF]

, 2005
Microfabricated integrated circuits revolutionized computation by vastly reducing the space, labor, and time required for calculations. Microfluidic systems hold similar promise for the large-scale automation of chemistry and biology, suggesting the ...
Quake, Stephen R., Squires, Todd M.
core   +1 more source

Structural Characterization and AI‐Enhanced Modeling of a Broadly Neutralizing Camelid Antibody Against SARS‐CoV‐2 Variants

Advanced Therapeutics, EarlyView.
This study characterizes a camelid antibody (B10) with potent neutralizing activity against SARS‐CoV‐2 Wildtype and Omicron variants. Through binding assays, epitope mapping, pseudovirus neutralization, and AI‐driven modeling, the antibody is shown to target conserved RBD regions in a closed Spike conformation.
Katja Hanack, Urszula Orzeł, Anja Schlör, Sourabh Mehta, Anandi Krishnan, Slawomir Filipek, Rushika Patel, Madhvi Joshi, Markus Hoffmann, Stefan Pöhlmann, Chaitanya Joshi, Dorian Liepmann, Ramasamy Paulmurugan, Venkatesan Renugopalakrishnan   +13 more
wiley   +1 more source

PlantID – DNA-based identification of multiple medicinal plants in complex mixtures [PDF]

, 2012
Background An efficient method for the identification of medicinal plant products is now a priority as the global demand increases. This study aims to develop a DNA-based method for the identification and authentication of plant species that can be ...
Bremner, Paul, Fowler, Mark, Graham, Eleanor, Howard, Caroline, Scott, Nigel, Slater, Adrian, Socratous, Eleni, Williams, Sarah   +7 more
core   +2 more sources

Integrin α8‐Mediated Pericyte Morphogenesis Controls Blood‐Brain Barrier Integrity

Advanced Science, EarlyView.
This study highlights that ITGA8 impacts pericyte morphology and function, both crucial for BBB integrity, via RhoA/ROCK signaling, thus influencing TGF‐β1 activation through cytoskeletal tension and ECM interactions. Notably, in post‐ischemic recovery models, ITGA8 deficiency exacerbates vascular dysfunction by impairing pericyte‐mediated BBB ...
Chang‐Xiong Gong, Lin‐Lin Hu, Ling Jiang, Pei‐Xia Shi, Shuang Zhang, Yuan Zhao, Bing‐Qiao Wang, Xiao‐Feng Cheng, Cheng‐Kang He, Sen Lin, Yue Dai, Zhao‐You Meng, Chen‐Hao Zhao, Bao‐Liang Sun, Qi Xie, Fang‐Fei Li, Qing‐Wu Yang   +16 more
wiley   +1 more source

Mono-\u3cem\u3eN\u3c/em\u3e-acyl-2,6-diaminopimelic Acid Derivatives: Analysis by Electromigration and Spectroscopic Methods and Examination of Enzyme Inhibitory Activity [PDF]

, 2014
Thirteen mono-N-acyl derivatives of 2,6-diaminopimelic acid (DAP)—new potential inhibitors of the dapE-encoded N-succinyl-l,l-diaminopimelic acid desuccinylase (DapE; EC 3.5.1.18)—were analyzed and characterized by infrared (IR) and nuclear magnetic ...
Buděšínský, Miloš, Gillner, Danuta M., Hlaváček, Jan, Holz, Richard C., Jiráček, Jiří, Kašička, Václav, Mikšík, Ivan, Pícha, Jan, Sázelová, Petra, Vaněk, Václav, Vítovcová, Miloslava   +10 more
core   +1 more source

A mutate-and-map protocol for inferring base pairs in structured RNA

, 2013
Chemical mapping is a widespread technique for structural analysis of nucleic acids in which a molecule's reactivity to different probes is quantified at single-nucleotide resolution and used to constrain structural modeling.
CS Chow, D Moazed, DH Mathews, EE Regulski, JB Lucks, JG Underwood, JP Staley, KA Wilkinson, M Kertesz, M Mandal, N Kulshina, NB Leontis, P Cordero, P Cordero, P Rocca-Serra, P Tijerina, PJ Lukavsky, R Rydzanicz, RC Spitale, RF Gesteland, S Mitra, S Washietl, S Yoon, SA Mortimer, SE Wells, SM Vasa, SW Brostoff, VD Sood, W Kladwang, W Kladwang, W Kladwang, W Kladwang, Y Rhee   +32 more
core   +1 more source

Okanin Suppresses the Growth of Colorectal Cancer Cells by Targeting at Peroxiredoxin 5

Advanced Science, EarlyView.
Okanin suppresses colorectal cancer growth by directly targeting PRDX5. This natural compound selectively binds peroxiredoxin 5 (PRDX5), inhibiting its activity and inducing WSB1‐mediated degradation. PRDX5 loss elevates ROS, suppresses GPX4 via SIAH2, and further triggers cell death (apoptosis and ferroptosis).
Ji Zhong Zhao, Yuan Fei Li, Fu Kang Yuan, Meng Lu Zhao, Ya Wen Han, Jia Xin Wang, Qi Yang, Han Ying Ye, Yu Cheng Lu, Shao Chin Lee   +9 more
wiley   +1 more source

Development of methods for capillary isoelectric focusing of dairy proteins : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Chemistry at Massey University [PDF]

, 2006
Capillary Isoelectric Focusing (CIEF) is a high-resolution technique which can be applied to the separation and characterisation of complex biological mixtures such as dairy proteins.
Gapper, Leyton William
core