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Two-Dimensional Difference Gel Electrophoresis
2018Two-dimensional difference gel electrophoresis (2D DIGE) is a modified form of 2D electrophoresis (2D E) that allows one to compare two or three protein samples simultaneously on the same gel. The proteins in each sample are covalently tagged with different color fluorescent dyes that are designed to have no effect on the relative migration of proteins
Malachi, Blundon +4 more
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Two-Dimensional Gel Electrophoresis Image Analysis
2021Gel-based proteomics is still quite widespread due to its high-resolution power; the experimental approach is based on differential analysis, where groups of samples (e.g., control vs diseased) are compared to identify panels of potential biomarkers.
Robotti E., Cala Elisa, Marengo E.
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Alignment of two-dimensional electrophoresis gels
Biochemical and Biophysical Research Communications, 2007Two-dimensional electrophoresis is a major separating technique for proteins in proteomics. Alignment of gel images is critical for intra-laboratory or even more difficult inter-laboratory gel comparisons. In the paper, we propose a novel iterative closest point (ICP) method for 2D-gel electrophoresis image alignment.
Shi, Guihua +4 more
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Two-Dimensional Difference Gel Electrophoresis
2012Two-dimensional difference gel electrophoresis (2D DIGE) is a modified form of 2D electrophoresis (2DE) that allows one to compare two or three protein samples simultaneously on the same gel. The proteins in each sample are covalently tagged with different color fluorescent dyes that are designed to have no effect on the relative migration of proteins ...
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Dialysis‐assisted two‐dimensional gel electrophoresis
ELECTROPHORESIS, 2006Abstract2‐DE is an important tool in proteomics research. However, intrinsic gel‐to‐gel variability of 2‐DE often masks the biological differences between the samples and compromises quantitative comparison of protein expression levels. Here, we describe a modification of 2‐DE that results in improved matching and quantification of proteins.
Olivier, Danos, Fedor, Svinartchouk
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Two-Dimensional Difference Gel Electrophoresis
2009The introduction of two-dimensional fluorescent difference gel electrophoresis has enabled the extensive screening of differential protein expression levels with higher confidence and greater sensitivity than using the classical two-dimensional electrophoresis (2DE) approach.
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Two-dimensional gel electrophoresis of proteins
Journal of Chromatography B: Biomedical Sciences and Applications, 1987The high-resolution capacity of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) makes it an excellent tool for the analysis and characterisation of complex protein mixtures. The evolution of two-dimensional electrophoresis is briefly described.
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High Resolution Two-dimensional Polyacrylamide-gel Electrophoresis
TrAC Trends in Analytical Chemistry, 1983Abstract The combination of two different gel electrophoretic techniques in a two-dimensional separation procedure provides the resolution capacity required for the simultaneous separation and analysis of complex protein mixtures. This technique is therefore a powerful tool for the study of phenotypic expression.
Michael J. Dunn, Arthur H.M. Burghes
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Two‐Dimensional Blue Native Polyacrylamide Gel Electrophoresis
Current Protocols in Cell Biology, 2008AbstractMultiprotein complexes play crucial roles in nearly all cell biological processes. Blue Native Polyacrylamide Gel Electrophoresis (BN‐PAGE) is a powerful method to study these complexes. It is a native protein separation method that relies on the dye Coomassie blue to confer negative charge for separation.
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Two-dimensional agarose gel electrophoresis without gel manipulation
Analytical Biochemistry, 1985The apparatus and procedure to perform two-dimensional agarose gel electrophoresis without manipulating the gel used for the first electrophoresis (first-dimension gel) have been developed. The procedure is less complex, less damaging to first-dimension gels, and more precise than procedures that require manipulation of the first-dimension gel.
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