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Two-dimensional agarose gel electrophoresis without gel manipulation
Analytical Biochemistry, 1985The apparatus and procedure to perform two-dimensional agarose gel electrophoresis without manipulating the gel used for the first electrophoresis (first-dimension gel) have been developed. The procedure is less complex, less damaging to first-dimension gels, and more precise than procedures that require manipulation of the first-dimension gel.
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Two-Dimensional Gel Electrophoresis-Based Proteomics of Mycobacteria
2009Two-dimensional gel electrophoresis (2-DE) in combination with mass spectrometry (MS) is the classic proteomics approach used to monitor the dynamics of protein abundance and posttranslational modifications in biological systems. In this chapter, we provide detailed protocols for 2-DE-based proteomics of mycobacteria.
Mattow, J. +5 more
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Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins
2003Since O'Farrell (1) introduced the improved technique for high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), it has become one of the most powerful tools for the separation and quantification of proteins from complex mixtures. The principal reason for this is that the method employs separation of denatured proteins according
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Two‐Dimensional Blue Native Polyacrylamide Gel Electrophoresis
Current Protocols in Cell Biology, 2008AbstractMultiprotein complexes play crucial roles in nearly all cell biological processes. Blue Native Polyacrylamide Gel Electrophoresis (BN‐PAGE) is a powerful method to study these complexes. It is a native protein separation method that relies on the dye Coomassie blue to confer negative charge for separation.
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Two-Dimensional Gel Electrophoresis and 2D-DIGE
2017Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) continues to be one of the most versatile and widely used techniques to study the proteome of a biological system. In particular, a modified version of 2D-PAGE, two-dimensional difference gel electrophoresis (2D-DIGE), which uses differential labeling of protein samples with up to three ...
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Universal strategies to multi-dimensional noble-metal-based catalysts for electrocatalysis
Coordination Chemistry Reviews, 2021Fei Gao, Yangping Zhang, Zhengying Wu
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Vapour-phase deposition of two-dimensional layered chalcogenides
Nature Reviews Materials, 2023Tianyi Zhang, Jiangtao Wang, Peng Wu
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Finite-temperature transport in one-dimensional quantum lattice models
Reviews of Modern Physics, 2021F Heidrich-Meisner +2 more
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