Results 211 to 220 of about 101,509 (256)

The role of bioinformatics in two‐dimensional gel electrophoresis

PROTEOMICS, 2003
Abstract Over the last two decades, two‐dimensional electrophoresis (2‐DE) gel has established itself as the de facto approach to separating proteins from cell and tissue samples.
Dowsey, Andrew W., Dunn, Michael J., Yang, Guang Zhong   +2 more
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Two-Dimensional Difference Gel Electrophoresis

2009
The introduction of two-dimensional fluorescent difference gel electrophoresis has enabled the extensive screening of differential protein expression levels with higher confidence and greater sensitivity than using the classical two-dimensional electrophoresis (2DE) approach.
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Two-dimensional gel electrophoresis of membrane proteins

Biochemistry, 1976
A high-resolution method for two-dimensional separation of membrane proteins is described. It involves a nondiscriminating solubilization of a membrane preparation with sodium dodecyl sulfate, followed by electrophoresis in the first dimension according to charge (by isoelectric focusing).
G F, Ames, K, Nikaido
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Detection of dystrophin on two-dimensional gel electrophoresis

Biochemical and Biophysical Research Communications, 1989
A protein with MW approximately 350 k daltons and pI approximately 5.5, which was deleted in the dystrophic mouse (C57BL/10ScSn-mdx), was detected on two-dimensional gel electrophoresis with silver staining. Deletion of this protein was uniformly observed in the dystrophic mouse extensor digitus longus, soleus and cardiac muscle.
S, Hori, H, Sugiura, T, Shimizu, T, Hirabayashi, S, Ohtani, M, Yoshida, K, Miyamoto, H, Tanabe   +7 more
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Sample preparation for two‐dimensional gel electrophoresis

PROTEOMICS, 2003
Abstract The choice of sample preparation protocol is a critical influential factor for isoelectric focusing which in turn affects the two‐dimensional gel result in terms of quality and protein species distribution. The optimal protocol varies depending on the nature of the sample for analysis and the properties of the constituent ...
Margaret M, Shaw, Beat M, Riederer
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Dialysis‐assisted two‐dimensional gel electrophoresis

ELECTROPHORESIS, 2006
Abstract2‐DE is an important tool in proteomics research. However, intrinsic gel‐to‐gel variability of 2‐DE often masks the biological differences between the samples and compromises quantitative comparison of protein expression levels. Here, we describe a modification of 2‐DE that results in improved matching and quantification of proteins.
Olivier, Danos, Fedor, Svinartchouk
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Preprocessing of two‐dimensional gel electrophoresis images

PROTEOMICS, 2004
Abstract Proteomics produces a huge amount of two‐dimensional gel electrophoresis images. Their analysis can yield a lot of information concerning proteins responsible for different diseases or new unidentified proteins. However, an automatic analysis of such images requires an efficient tool for reducing noise in
Krzysztof, Kaczmarek, Beata, Walczak, Sijmen, de Jong, Bernard G M, Vandeginste   +3 more
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Two-Dimensional Gel Electrophoresis: A Reference Protocol

2017
Two-dimensional gel electrophoresis (2DE) has been a mainstay of proteomic techniques for more than four decades. It was even in use for several years before the term proteomics was actually coined in the early 1990s. Over this time, it has been used in the study of many diseases including cancer, diabetes, heart disease, and psychiatric disorders ...
Veronica M, Saia-Cereda, Adriano, Aquino, Paul C, Guest, Daniel, Martins-de-Souza   +3 more
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Automatic segmentation and modelling of two-dimensional electrophoresis gels

Proceedings of 3rd IEEE International Conference on Image Processing, 2002
An important issue in the analysis of two-dimensional electrophoresis images is the detection and quantification of protein spots. In this paper we describe a new robust technique to segment and model the different spots present in the gels. For the segmentation a watershed technique is applied.
Eva Bettens, Paul Scheunders, Jan Sijbers, Dirk Van Dyck, L. Moens   +4 more
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Two-Dimensional Gel Electrophoresis of Total Yeast Proteins

2005
Two-dimensional gel electrophoresis (2-DE) offers the opportunity of separating several hundred proteins from a total yeast cellular extract. A detailed description is provided here of the different steps required for the separation and visualization of radiolabeled yeast proteins on high-resolution (24 cm x 20 cm) 2-D gels.
Boucherie, H., Monribot-Espagne, C.
openaire   +3 more sources