Results 21 to 30 of about 101,509 (256)

Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry

Biological Procedures Online, 2009
The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass ...
Mandal Nakul, Heegaard Steffen, Prause Jan, Honoré Bent, Vorum Henrik   +4 more
doaj   +1 more source

Two-dimensional gel electrophoresis in bacterial proteomics [PDF]

Protein & Cell, 2012
Two-dimensional gel electrophoresis (2-DE) is a gel-based technique widely used for analyzing the protein composition of biological samples. It is capable of resolving complex mixtures containing more than a thousand protein components into individual protein spots through the coupling of two orthogonal biophysical separation techniques: isoelectric ...
Watt, RM, Lau, SKP, Woo, PCY, Curreem, SOT   +3 more
openaire   +4 more sources

Application of Fluorescence Two-Dimensional Difference In-Gel Electrophoresis as a Proteomic Biomarker Discovery Tool in Muscular Dystrophy Research

Biology, 2013
In this article, we illustrate the application of difference in-gel electrophoresis for the proteomic analysis of dystrophic skeletal muscle. The mdx diaphragm was used as a tissue model of dystrophinopathy.
Steven Carberry, Margit Zweyer, Dieter Swandulla, Kay Ohlendieck   +3 more
doaj   +1 more source

Comparative 2-DE protein analysis in a 3-D geometry gel

BioTechniques, 2007
Two-dimensional gel electrophoresis (2-DE) separation has not been considered suitable for large-scale comparative protein expression studies due to its limited throughput.
Robert Ventzki, Sabrina Rüggeberg, Stefan Leicht, Thomas Franz, Josef Stegemann   +4 more
doaj   +1 more source

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, Volume 20, Issue 6, Page 1429-1452, June 2026.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source

Protein profile analysis of Malaysian snake venoms by two-dimensional gel electrophoresis

Journal of Venomous Animals and Toxins including Tropical Diseases, 2010
Snake venoms comprise a highly complex mixture of proteins, which requires for their characterization the use of versatile two-dimensional electrophoresis techniques.
J Vejayan, L Shin Yee, G Ponnudurai, S Ambu, I Ibrahim   +4 more
doaj   +1 more source

Dataset of Two-Dimensional Gel Electrophoresis Images of Acute Myeloid Leukemia Patients before and after Induction Therapy

Data, 2021
Acute myeloid leukemia (AML) is a malignant disorder of the hematopoietic stem and progenitor cells, which results in the build-up of immature blasts in the bone marrow and eventually in the peripheral blood of affected patients.
Juan E. Urrea, Luisa F. Restrepo, Jeanette Prada-Arismendy, Erwing Castillo, Manuel M. Goez, Maria C. Torres-Madronero, Edilson Delgado-Trejos, Sarah Röthlisberger   +7 more
doaj   +1 more source

Proteasome inhibitor, ixazomib prevents topoisomerase‐I degradation and reverses irinotecan resistance in colorectal cancer

Molecular Oncology, EarlyView.
Ixazomib inhibits proteasome‐mediated degradation of topoisomerase I induced by irinotecan, thereby restoring drug sensitivity and promoting tumor cell death in colorectal cancer. Irinotecan, a topoisomerase I (topoI) inhibitor, is widely used for colorectal cancer, but resistance remains a major clinical challenge.
Yuho Ebata, Koji Ando, Hirofumi Hasuda, Koshi Mimori, Elizabeth C. Unan, Siddhartha Pulukuri, Aahana Tiku, Allison Berger, Eiji Oki, Ajit Bharti, Tomoharu Yoshizumi   +10 more
wiley   +1 more source

Utility of Two-Dimensional Difference Gel Electrophoresis in Diagnosis of Multiple Sclerosis

Diagnostics, 2018
Two-dimensional difference gel electrophoresis (2D-DIGE) has been used for identification of possible biomarkers in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients.
Michael Auer, Harald Hegen, Dagmar Rudzki, Georg Golderer, Florian Deisenhammer   +4 more
doaj   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source