Results 21 to 30 of about 705,442 (212)
Silver Staining of 2D Electrophoresis Gels [PDF]
Methods in Molecular Biology -Clifton then Totowa- 893 (2012)
61-73, 2012 Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It -combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in proteomics, two important additional features must be considered, compatibility with mass spectrometry and ...
arxiv +1 more source
Genomic and proteomic profiling for cancer diagnosis in dogs [PDF]
, 2016 Global gene expression, whereby tumours are classified according to similar gene expression patterns or ‘signatures’ regardless of cell morphology or tissue characteristics, is being increasingly used in both the human and veterinary fields to assist in ...
Morris, Joanna S.
core +1 more source
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
Biochemical Journal, 1967 1. Gels were prepared with recrystallized acrylamide and bisacrylamide. Electrophoresis was in tris-sodium acetate-EDTA buffer for 0.5 to 3hr. Gels were scanned at 280 or 265mmu. Techniques are described for slicing and radioactive counting. 2.
U. Loening
semanticscholar +1 more source
Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters
BioTechniques, 2023 Atomically precise nanoclusters comprising 1–100 atoms have emerged as a new class of nanomaterials with intriguing size-dependent physicochemical properties. The significant changes in the properties of nanoclusters were observed in tailoring the number
Vinitha Packirisamy, Prabhu Pandurangan
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Purification and characterisation of a carboxylesterase from the latex ofSynadenium grantii Hook, ‘f’ [PDF]
, 1987 The latex ofSynadenium grantii was found to contain esterolytic activity. Polyacrylamide gel electrophoretic study coupled with substrate and inhibitor specificity studies revealed the presence of multiple forms of carboxylesterases and cholinesterases ...
Govardhan,, L. +3 more
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, 1966 Fractionation of normal cerebrospinal fluid proteins was carried out by Disc-electrophoresis and the obtained patterns were compared with those of normal sera.Protein fractions of cerebrospinal fluid were named provisionally as follows:Prealbumin (ρ ...
Y. Asagami
semanticscholar +1 more source
A versatile polyacrylamide gel electrophoresis based sulfotransferase assay
BMC Biotechnology, 2010 Background Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known
Prather Brittany +4 more
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A two-dimensional electrophoresis protocol suitable for Medicago truncatula leaf proteome [PDF]
, 2015 Medicago truncatula leaves were used as the experimental materials. Total proteins of leaves were extracted by trichloracetic acid (TCA)-acetone method and proteins had a better separation using gel strips, forming an immobilized non-linear 3 to 10 pH ...
Dong, Wen +7 more
core +2 more sources
Proceedings of the National Academy of Sciences of the United States of America, 1989 We developed mobility shift analysis of single-stranded DNAs on neutral polyacrylamide gel electrophoresis to detect DNA polymorphisms. This method follows digestion of genomic DNA with restriction endonucleases, denaturation in alkaline solution, and ...
Masato Orita +4 more
semanticscholar +1 more source
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives
BioTechniques, 2008 The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
doaj +1 more source