Results 21 to 30 of about 712,932 (237)

Conducting polymer electrodes for gel electrophoresis.

open access: yesPLoS ONE, 2014
In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that π-conjugated polymers such as poly(3,4-
Katarina Bengtsson   +2 more
doaj   +1 more source

Silver Staining of Proteins in 2DE Gels [PDF]

open access: yes, 2009
Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals.
Chevallet, Mireille   +3 more
core   +4 more sources

ELECTROPHORETIC SYSTEMS FOR PREPARATIVE FRACTIONATION OF PROTEIN PRECURSORS OF BIOACTIVE PEPTIDES FROM COW’S MILK

open access: yesHarčova Nauka ì Tehnologìâ, 2018
The article considers the possibility of obtaining purified fractions-precursors of bioactive peptides from milk proteins by the method of preparative electrophoresis.
V. Yukalo   +3 more
doaj   +1 more source

Analysis of chitosan molecular weight profile by electrophoresis in a porosity step gradient polyacrylamide gel [PDF]

open access: yesThe Ukrainian Biochemical Journal, 2022
Chitosan is biocompatible and biodegradable natural biopolymer widely applied in various fields of biology, medicine, and pharmacy, however, its effects significantly depend on the degree of polymerization (DP) and the degree of deacetylation (DDA) of ...
N. O. Manko   +3 more
doaj   +1 more source

Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia [PDF]

open access: yes, 1971
Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide ...
Wilson, David L.
core   +4 more sources

Preparative electrophoresis of human apolipoprotein E: an improved method.

open access: yesJournal of Lipid Research, 1990
Apolipoprotein E was isolated from human very low density lipoproteins by a two-step electrophoretic procedure derived from that of Méndez (1982. Anal. Biochem. 126: 403-408).
S Meunier   +3 more
doaj   +1 more source

Comparison of the modified polyacrylamide gradient gel electrophoresis and high-performance liquid chromatography methods in determining LDL size

open access: yesTürk Biyokimya Dergisi, 2022
In this study, we aimed to compare modified Krauss polyacrylamide gradient gel electrophoresis (PAGGE) and high-performance liquid chromatography (HPLC) methods in classification, quantification, and separation of lipoproteins and determining low-density
Cihan Murat   +3 more
doaj   +1 more source

Membrane proteins and proteomics: Love is possible, but so difficult [PDF]

open access: yes, 2009
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi   +103 more
core   +4 more sources

A novel mechanism of "metal gel-shift" by histidine-rich Ni2+-binding Hpn protein from Helicobacter pylori strain SS1. [PDF]

open access: yesPLoS ONE, 2017
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is a universally used method for determining approximate molecular weight (MW) in protein research.
Rahul Mahadev Shelake   +4 more
doaj   +1 more source

Characterization of the aerosol produced by infrared femtosecond laser ablation of polyacrylamide gels for the sensitive inductively coupled plasma mass spectrometry detection of selenoproteins [PDF]

open access: yes, 2009
A 2D high repetition rate femtosecondlaserablation strategy (2-mm wide lane) previously developed for the detection of selenoproteins in gel electrophoresis by inductively coupled plasma mass spectrometry was found to increase signal sensitivity by a ...
Alexis, Joël   +7 more
core   +2 more sources

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