Results 71 to 80 of about 560,418 (257)

Kidney cell electrophoresis [PDF]

open access: yes
The following aspects of kidney cell electrophoresis are discussed: (1) the development and testing of electrophoresis solutions; (2) optimization of freezing and thawing; (3) procedures for evaluation of separated kidney cells; and (4) electrophoretic ...
Todd, P.
core   +4 more sources

Strongly nonlinear waves in capillary electrophoresis

open access: yes, 2012
In capillary electrophoresis, sample ions migrate along a micro-capillary filled with a background electrolyte under the influence of an applied electric field.
F. Kohlrausch   +4 more
core   +1 more source

Establishment and biological characterization of radioresistant colorectal cancer cell lines

open access: yesFEBS Open Bio, EarlyView.
Under ionizing radiation exposure, radiation‐sensitive cancer cells exhibit oxidative stress and DNA damage, while radiation‐resistant cancer cells exhibit strong antioxidant properties and DNA damage repair. Radiotherapy resistance is a major cause of recurrence and metastasis in colorectal cancer (CRC).
Tian‐Yin Qu   +10 more
wiley   +1 more source

Capillary electrophoresis characterisation of a rapid prototyped PMMA chip for particle analysis [PDF]

open access: yes, 2010
Màster en Nanociència i NanotecnologiaA rapid and cheap method has been developed for the fabrication of a capillary electrophoresis chip for the preliminary characterization of particles.
Colom, Adai
core   +1 more source

Hydrophobicity causes anomalous migration of cystine/glutamate antiporter SLC7A11 in SDS‐PAGE with low acrylamide concentration

open access: yesFEBS Open Bio, EarlyView.
SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel   +13 more
wiley   +1 more source

Intracellular protein crystallization in living insect cells

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides a step‐by‐step guide for applying the InCellCryst pipeline for intracellular protein crystallization. After gene cloning and generation of recombinant baculoviruses, High Five insect cells are infected for target protein crystallization.
Robert Schönherr   +8 more
wiley   +1 more source

Analysis of water-soluble vitamins in biopharma raw materials by electrophoresis micro-chips with contactless conductivity detection [PDF]

open access: yes, 2009
Detailed information concerning the composition of the raw materials employed in the production of biologics is important for the efficient control and optimization of bioprocesses.
Benito-Lopez, Fernando   +3 more
core  

Equi‐MOI ratio for rapid baculovirus‐mediated multiprotein co‐expression in insect cells integrating selenomethionine for structural studies

open access: yesFEBS Open Bio, EarlyView.
This study presents a versatile protocol for producing selenomethionine‐labeled multiprotein complexes, addressing challenges in structural biology such as experimental phasing in X‐ray crystallography. Its efficiency and adaptability make it valuable for researchers studying de novo, dynamic, or complex protein structures, promoting advancements in ...
Andrej Bitala   +3 more
wiley   +1 more source

Electrophoresis simulated with the cage model for reptation [PDF]

open access: yesarXiv, 1999
The cage model for polymer reptation is extended to simulate DC electrophoresis. The drift velocity v of a polymer with length L in an electric field with strength E shows three different regions: if the strength of field is small, the drift velocity scales as E/L; for slightly larger strengths, it scales as E^2, independent of length; for high fields,
arxiv  

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