Results 111 to 120 of about 192,083 (323)

A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall [PDF]

, 2019
Yuichi Furuhata, Ayako Sakai, Tomi Murakami, Mone Morikawa, Chikashi Nakamura, Takeshi Yoshizumi, Ushio Fujikura, Keiji Nishida, Yoshio Kato   +8 more
openalex   +1 more source

Calcium Electroporation Reduces Viability and Proliferation Capacity of Four Uveal Melanoma Cell Lines in 2D and 3D Cultures [PDF]

, 2022
Miriam M. Kraemer, Theodora Tsimpaki, Utta Berchner‐Pfannschmidt, Nikolaos E. Bechrakis, Berthold Seitz, Miltiadis Fiorentzis   +5 more
openalex   +1 more source

Two Novel S‐methyltransferases Confer Dimethylsulfide Production in Actinomycetota

Advanced Science, EarlyView.
This study identifies two novel S‐adenosine‐methionine‐dependent methyltransferases, MddM1 and MddM2, in actinomycetes from the Mariana Trench. These enzymes can convert toxic hydrogen sulfide (H2S) and methanethiol (MeSH) into dimethylsulfide (DMS), serving as a cellular detoxification and oxidative stress response.
Ruihong Guo, Zihua Guo, Yi Zhou, Yunhui Zhang, Haojin Cheng, Rebecca Devine, Chuang Sun, Ronghua Liu, Yanfen Zheng, Andrew J. Gates, Jonathan D. Todd, Xiao‐Hua Zhang   +11 more
wiley   +1 more source

Modeling of morphologically realistic large-scale multicellular electroporation for population-level analysis

Results in Engineering
This study explores the dynamics of multicellular electroporation within a monolayer cluster of HCMEC/D3 cells subjected to pulsed electric fields.
Lujia Ding, Mike A.J. Moser, Chris Zhang, Ningning Hu   +3 more
doaj   +1 more source

Diffusion-Weighted MRI for Verification of Electroporation-Based Treatments [PDF]

, 2011
Faisal Mahmood, Rasmus Hvass Hansen, Birgit Agerholm-Larsen, Kurt Svarre Jensen, Helle K. Iversen, Julie Gehl   +5 more
openalex   +1 more source

Promoting Autophagy Mitigates Stress‐Induced Remodeling in Patient iPSC‐CMs with the Phospholamban R9C Mutation

Advanced Science, EarlyView.
The Phospholamban (PLN) R9C mutation reduces SERCA2a binding, increasing calcium recycling and baseline contractility. However, the excess of free PLN promotes pentamer formation, limiting phosphorylation and blunting β‐adrenergic signaling. Under cardiac stress, enhanced functional demands overwhelm proteostasis in PLN R9C cells, leading to misfolded ...
Qi Yu, Yawei Shen, Robert J. Barndt, Karim Sallam, Ying Tang, Cameron E. Brown, Xiao Li, Stephen Y. Chan, Joseph C. Wu, Qing Liu, Haodi Wu   +10 more
wiley   +1 more source

Enhancement effect of urea toward electroporation-mediated plasmid transfection efficiency in the HEK-293 cell line

Research in Pharmaceutical Sciences
Intracellular delivery is crucial in biological and medical studies. Although many molecular tools have been created for cell-based gene therapies, it remains challenging to introduce external molecules into cells.
Mahshid Mowla, Gilar Gorji-Bahri, Hamid Reza Moghimi, Atieh Hashemi   +3 more
doaj   +1 more source

Reversible Electroporation–Mediated Liposomal Doxorubicin Delivery to Tumors Can Be Monitored With Zr-Labeled Reporter Nanoparticles

Molecular Imaging, 2018
Reversible electroporation (RE) can facilitate nanoparticle delivery to tumors through direct transfection and from changes in vascular permeability. We investigated a radiolabeled liposomal nanoparticle ( 89 Zr-NRep) for monitoring RE-mediated liposomal
Govindarajan Srimathveeravalli PhD, Dalya Abdel-Atti BS, Carlos Pérez-Medina PhD, Haruyuki Takaki MD, PhD, Stephen B. Solomon MD, Willem J. M. Mulder PhD, Thomas Reiner PhD   +6 more
doaj   +1 more source

Optically-controlled platforms for transfection and single- and sub-cellular surgery [PDF]

, 2015
Improving the resolution of biological research to the single- or sub-cellular level is of critical importance in a wide variety of processes and disease conditions.
Casey, DR, Glückstad, J, Villangca, M
core  

Unraveling the Morphological and Functional Maturation Mechanisms Underlying Human Neural Development Using iPSCs‐Derived Neuronal Model

Advanced Science, EarlyView.
Using human induced pluripotent stem cells (hiPSCs)‐derived neuronal model, Tian and colleagues reveal that voltage‐gated calcium channels Cav1.2 and Cav1.3, and their mediated calcium ion influx, are essential for early morphogenesis of human neuronal development, while ECEL1 underlies human neuronal functional developmental maturation through CALM3 ...
Yue Tian, Yi‐Chun Ou, Zi‐Xian Zhang, Jie Cai, Si‐Qing Cai, Guo‐Gang Xing   +5 more
wiley   +1 more source