Results 121 to 130 of about 135,255 (300)
High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation
, 2014 We established a high-efficiency nuclear transformation method for the diatom Phaeodactylum tricornutum using an electroporation system. Based on a universal electroporation protocol, the conditions for the introduction of exogenous DNA including ...
Zhang, Chunye, Hu, Hanhua
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A Pooled CRISPR Screen Reveals Genes Critical for Erythroblast Enucleation
American Journal of Hematology, EarlyView.ABSTRACT Terminal erythroid differentiation involves dramatic cellular remodeling that culminates in the expulsion of the nucleus, a process known as enucleation. While enucleation is conserved across mammals and is crucial for the generation of fully functional erythrocytes, the mechanisms governing this process have remained largely unknown, in part ...
Marilou Tetard +7 more
wiley +1 more source
Stable transformation of sugar beet protoplasts by electroporation.
, 1989 Conditions were optimized for the culture, antibiotic selection and stable transformation by electroporation of suspension culture protoplasts of sugarbeet,Beta vulgaris L..
Jones, M.G.K. +2 more
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Use of electroporation and reverse iontophoresis for extraction of transdermal multibiomarkers [PDF]
, 2012 Congo Tak-Shing Ching1,2, Lin-Shien Fu3-5, Tai-Ping Sun1, Tzu-Hsiang Hsu1, Kang-Ming Chang21Department of Electrical Engineering, National Chi Nan University, Puli, Nantou County, 2Department of Photonics and Communication Engineering, Asia University ...
Sun TP +4 more
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Electroporation Optimization: A User’s Guide
, 2018 Electroporation is an effective physical method for delivery of various types and sizes of molecules to cells in culture. It is a fast and relatively easy way to re-engineer cells to contain exogeneous nucleic acids or small molecules.
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Irreversible Electroporation for Inferior Turbinate Reduction Ablation in Adults
International Forum of Allergy &Rhinology, EarlyView.ABSTRACT Background Inferior turbinate hypertrophy is a common cause of nasal airway obstruction. An optimal surgical approach should provide effective volume reduction while preserving mucosal integrity and minimizing postoperative morbidity. Objective To evaluate the safety and efficacy of irreversible electroporation (IRE) for non‐thermal ablation ...
Narin Nard Carmel Neiderman +9 more
wiley +1 more source
Angewandte Chemie International Edition, EarlyView.This study demonstrates a novel hybrid system that integrates electrochemical CO2 reduction with microbial biosynthesis in a single reactor, using a synthetic autotrophic yeast to produce itaconic acid from CO2. Using an industrial yeast provides feasibility of direct electromicrobial CO2 conversion to bio‐based building blocks while identifying key ...
Paniz Izadi +9 more
wiley +1 more source
Electroporation induced protein elution out to extracellular media and cytoplasmic membrane blebbing
Electroporation, a method that uses electrical pulses to temporarily permeabilize cell membranes, has emerged as a useful tool in cell biology and biotechnology.
Jakštys, Baltramiejus +2 more
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The Role of miRNAs in Chicken Immune Regulation and Prospects for Disease‐Resistant Breeding
Animal Research and One Health, EarlyView.A schematic workflow illustrating the screening of disease‐resistant miRNAs and the generation of miRNA‐based disease‐resistant chickens via PGC‐mediated germline genome editing. ABSTRACT MicroRNAs (miRNAs) are emerging as pivotal regulators of the immune system, playing a decisive role in shaping disease resistance in chicken.
Qiangzhou Wang +10 more
wiley +1 more source
626 Improving the yeast transformation efficiency for yeast display in antibody development
Journal for ImmunoTherapy of Cancer, 2020 Jian Chen, George Sun
doaj +1 more source