Results 141 to 150 of about 135,255 (300)

PENGARUH TEGANGAN ELEKTROPORASI TERHADAP EFISIENSI TRANSFORMASI PLAMID pND968 BAKTERI ASAM LAKTAT KE E. coli HB101 [The Effect of Electroporation Voltage on Transformation Efficiency of Plasmid pND968 of Lactic Acid Bacteria Into E. coli HB101]

Jurnal Teknologi dan Industri Pangan, 2012
The research was conducted to examine the effect of different electroporation voltage on transformation efficiency of plasmid pND968. This was carried out by exposing the mixture of plasmid pND968 and competent cells of E.
Widodo .
doaj  

Efficient transformation and genome editing in a nondomesticated, biocontrol strain, Bacillus subtilis GLB191

Phytopathology Research
Bacillus subtilis has been widely used as a biological control agent in agricultural production. Environmental strains of B. subtilis are an important source of biological control agents.
Yu Zhao, Zhenshuo Wang, Qian Wang, Bing Wang, Xiaoning Bian, Qingchao Zeng, Daowan Lai, Qi Wang, Yan Li   +8 more
doaj   +1 more source

Research on the behavior of annexin A4 protein after cell electroporation: insights into the active membrane repair mechanisms

Increase in cell membrane permeability after cell treatment with short, high-voltage electric pulses (electroporation) is related with the formation of hydrophilic pores and ROS induced phospholipid oxidation.
Jakštys, Baltramiejus, Makarovas, Dominykas, Šatkauskas, Saulius   +2 more
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Electroporation of the Skin

, 2010
Transdermal drug delivery offers an attractive alternative to the conve ntional drug delivery methods of oral administration and injection. However, the stratum corneum acts as a barrier that limits the penetration of substances through the skin ...
Cesar A. Gonzalez, Boris Rubinsky
core   +1 more source

Extracellular Vesicles‐Based Biological Macromolecule Delivery Systems: Biogenesis, Characterization, and Emerging Clinical Applications

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Extracellular vesicles (EVs) are nanoscale membrane‐bound structures that play a pivotal role in intercellular communication by transporting bioactive molecules, including proteins, lipids, nucleic acids, and organelles, between cells. Originating from the endosomal pathway, EVs reflect the physiological and pathological status of their parent
Emine İncilay Torunoğlu, Zeynep Betül Sarı, Muhammed Emin Sarı, Erdi Can Aytar   +3 more
wiley   +1 more source

Combinatorial Metabolic Engineering of Alkane Biosynthesis in the Osmotolerant Yeast Debaryomyces hansenii CBS 767

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Debaryomyces hansenii is a yeast with diverse potential applications like producing oleochemicals from biomass. Yet, combinatorial metabolic engineering in D. hansenii is limited because, like many nonconventional organisms, key genomic data and genetic elements are missing. Here, we report phenotypic characterization, genomic integration loci,
Zekun Li, Nilesh Kumar Sharma, Sarah Weintraub, Eric Young   +3 more
wiley   +1 more source

Unmasking Glycoforms: Lectin‐Based Profiling and Functional Implications of Targeted Glycosylation Knockouts in Chinese Hamster Ovary Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody (mAb) glycosylation is a critical quality attribute that is difficult to rationally engineer and rapidly assess during cell line development. Here, we investigate whether cell‐surface glycosylation can serve as a surrogate readout of mAb product glycosylation following targeted glycogene engineering in CHO cells.
Cristina Abascal Ruiz, Sheryl Li Yan Lim, Jacobus Brink, Sara Carillo, Eoin Casey, Jonathan Bones, Ioscani Jiménez del Val   +6 more
wiley   +1 more source

Optimizing electroporation: efficiency and cell viability in the simultaneous transfer of bovine serum albumin, propidium iodide and nucleic acids

The delivery of exogenous proteins into cells via electroporation has been used for biological inquiries, such as CRISPR-Cas9 gene editing and intracellular signaling detection.
Barauskaitė-Šarkinienė, Neringa, Venckus, Justinas, Jakštys, Baltramiejus, Vykertas, Salvijus, Urbanskas, Ernestas, Ruzgys, Paulius, Šatkauskas, Saulius   +6 more
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Cell and Gene Therapies Manufacturing Challenges and Integrated Good Manufacturing Practices Solutions: A Lifecycle Perspective

Biotechnology and Bioengineering, EarlyView.
Lifecycle perspective on cell and gene therapy manufacturing challenges and enabling GMP solutions. ABSTRACT Cell and gene therapies (CGTs) are revolutionizing modern medicine; however, making these advanced medicines scalable and readily available to commercial manufacturers worldwide is a major challenge. The number of approved CGT products continues
Rajath Samaga, Udisha Singh, Sachin Dattram Pawar   +2 more
wiley   +1 more source

TeraHertz Spectroscopy for Electroporation

, 2017
The application of intense, short-duration pulsed electric fields to mammalian cells causes an increase in the permeability of plasma membrane to external molecules (electroporation).
Romeo S
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