Results 151 to 160 of about 133,618 (201)

Proteomic Differentiation of Colorectal Cancer From Normal Tissue via Ex Vivo E-Biopsy: A Novel Approach to Molecular Sampling for Diagnostic Precision. [PDF]

open access: yesCancer Med
Saulis G   +9 more
europepmc   +1 more source

Transfection by Electroporation

Current Protocols in Molecular Biology, 2010
AbstractElectroporation—the use of high‐voltage electric shocks to introduce DNA into cells—can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
Huntington, Potter, Richard, Heller
openaire   +6 more sources

Electroporation of the heart

EP Europace, 2005
Abstract Defibrillation shocks are commonly used to terminate life-threatening arrhythmias. According to the excitation theory of defibrillation, such shocks are aimed at depolarizing the membranes of most cardiac cells resulting in resynchronization of electrical activity in the heart. If shock-induced changes in transmembrane potential
Vladimir P, Nikolski, Igor R, Efimov
openaire   +2 more sources

Electroporation of Mycobacteria

2021
The introduction of DNA into bacterial cells is one of the foundational methods of bacterial genetics. Transformation of mycobacterial species is complicated due to the structure of the cell wall, which has a complex outer layer with low permeability. Electroporation has become a routine procedure in genetic studies.
openaire   +2 more sources

Electroporation of Mycobacteria

2015
High-efficiency transformation of DNA is integral to the study of mycobacteria, allowing genetic manipulation. Electroporation is the most widely used method for introducing DNA into mycobacterial strains. Many parameters contribute to high-efficiency transformation; these include the species per strain, the transforming DNA, the selectable marker, the
Goude, Renan   +2 more
openaire   +2 more sources

Electroporation of Mycobacteria

2003
High-efficiency transformation is a major limitation in the study of mycobacteria. The genus Mycobacterium can be difficult to transform; this is mainly caused by the thick and waxy cell wall but is compounded by the fact that most molecular techniques have been developed for distantly related species such as Escherichia coli and Bacillus subtilis.
T, Parish, N G, Stoker
openaire   +3 more sources

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