Results 11 to 20 of about 192,083 (323)
Cold Spring Harbor Protocols, 2019 Electroporation is a process in which brief electrical pulses create transient pores in the plasma membrane that allow nucleic acids to enter the cellular cytoplasm. Here, we provide information on the history, mechanism, and optimization of electroporation.
Priti, Kumar +2 more
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Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation
Sensors, 2022 A specific pulsed electric field protocol can be used to induce electroporation. This is used in the food industry for yeast pasteurization, in laboratories for generic transfer and the medical field for cancer treatment.
Guilherme B. Pintarelli +3 more
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APL Bioengineering, 2023 Electroporation shows great potential in biology and biomedical applications. However, there is still a lack of reliable protocol for cell electroporation to achieve a high perforation efficiency due to the unclear influence mechanism of various factors,
Qiongyao Mou +4 more
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Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel
Micromachines, 2020 The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics ...
Yifei Ye +7 more
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Recent electroporation-based systems for intracellular molecule delivery
Nanotechnology and Precision Engineering, 2021 Intracellular delivery of functional molecules, such as DNA probes and plasmids, is an important method for investigating cellular mechanisms and changing cell fates in biomedicine.
Zaizai Dong, Lingqian Chang
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Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts
BMC Molecular and Cell Biology, 2020 Background Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than ...
Iqbal Hyder +2 more
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The Journal of Reproduction and Development, 2022 Genetically engineered animals can be produced quickly using genome editing technology. A new electroporation technique, technique for animal knockout system by electroporation (TAKE), aids in the production of genome-edited animals by introducing ...
Yui WAKE +3 more
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Irreversible Electroporation [PDF]
Seminars in Interventional Radiology, 2015 Irreversible electroporation (IRE) is a predominantly nonthermal ablative technology that uses high-voltage, low-energy DC current pulses to induce cell death. Thermal ablative technologies such as radiofrequency ablation, microwave ablation, and cryoablation have several applications in oncology but have limitations that have been established. IRE has
openaire +2 more sources
Synergistic Combination of Electrolysis and Electroporation for Tissue Ablation. [PDF]
PLoS ONE, 2016 Electrolysis, electrochemotherapy with reversible electroporation, nanosecond pulsed electric fields and irreversible electroporation are valuable non-thermal electricity based tissue ablation technologies. This paper reports results from the first large
Michael K Stehling +5 more
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Biomolecules, 2023 Electroporation is a biophysical phenomenon involving an increase in cell membrane permeability to molecules after a high-pulsed electric field is applied to the tissue.
Maria Scuderi +8 more
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