Results 241 to 250 of about 182,543 (292)

Nuclear and cytoplasmic remodeling of ectocervical cells by high activity of gelsolin. [PDF]

open access: yesSci Rep
Farkas B   +8 more
europepmc   +1 more source

Systematic Optimization of 293T Cell Electroporation: Balancing High-Efficiency Gene Delivery with Cell Viability

open access: yes
Zhang M   +11 more
europepmc   +1 more source

Transfection by Electroporation

Current Protocols in Molecular Biology, 2010
AbstractElectroporation—the use of high‐voltage electric shocks to introduce DNA into cells—can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
Huntington, Potter, Richard, Heller
openaire   +6 more sources

Electroporation of Mycobacteria

2021
The introduction of DNA into bacterial cells is one of the foundational methods of bacterial genetics. Transformation of mycobacterial species is complicated due to the structure of the cell wall, which has a complex outer layer with low permeability. Electroporation has become a routine procedure in genetic studies.
openaire   +2 more sources

Single-cell electroporation

Pflügers Archiv - European Journal of Physiology, 2001
Using modified patch-clamp methodology, we demonstrated that it is possible to insert genes or other compounds routinely into single cells by electroporation. When the cell is indented by a small-tipped microelectrode, a voltage of 10 V or less in the pipette is divided by the pipette resistance and the series resistance of the cleft between the ...
James L, Rae, Richard A, Levis
openaire   +2 more sources

Single-cell electroporation

Current Opinion in Biotechnology, 2003
Electroporation is a widely used method for the introduction of polar and charged agents such as dyes, drugs, DNA, RNA, proteins, peptides, and amino acids into cells. Traditionally, electroporation is performed with large electrodes in a batch mode for treatment of a large number of cells in suspension.
Jessica, Olofsson   +5 more
openaire   +2 more sources

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