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Transfection by Electroporation
Current Protocols in Molecular Biology, 2010AbstractElectroporation—the use of high‐voltage electric shocks to introduce DNA into cells—can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
Huntington, Potter, Richard, Heller
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EP Europace, 2005
Abstract Defibrillation shocks are commonly used to terminate life-threatening arrhythmias. According to the excitation theory of defibrillation, such shocks are aimed at depolarizing the membranes of most cardiac cells resulting in resynchronization of electrical activity in the heart. If shock-induced changes in transmembrane potential
Vladimir P, Nikolski, Igor R, Efimov
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Abstract Defibrillation shocks are commonly used to terminate life-threatening arrhythmias. According to the excitation theory of defibrillation, such shocks are aimed at depolarizing the membranes of most cardiac cells resulting in resynchronization of electrical activity in the heart. If shock-induced changes in transmembrane potential
Vladimir P, Nikolski, Igor R, Efimov
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Electroporation of Mycobacteria
2021The introduction of DNA into bacterial cells is one of the foundational methods of bacterial genetics. Transformation of mycobacterial species is complicated due to the structure of the cell wall, which has a complex outer layer with low permeability. Electroporation has become a routine procedure in genetic studies.
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Electroporation of Mycobacteria
2015High-efficiency transformation of DNA is integral to the study of mycobacteria, allowing genetic manipulation. Electroporation is the most widely used method for introducing DNA into mycobacterial strains. Many parameters contribute to high-efficiency transformation; these include the species per strain, the transforming DNA, the selectable marker, the
Goude, Renan +2 more
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Electroporation of Mycobacteria
2003High-efficiency transformation is a major limitation in the study of mycobacteria. The genus Mycobacterium can be difficult to transform; this is mainly caused by the thick and waxy cell wall but is compounded by the fact that most molecular techniques have been developed for distantly related species such as Escherichia coli and Bacillus subtilis.
T, Parish, N G, Stoker
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Pflügers Archiv - European Journal of Physiology, 2001
Using modified patch-clamp methodology, we demonstrated that it is possible to insert genes or other compounds routinely into single cells by electroporation. When the cell is indented by a small-tipped microelectrode, a voltage of 10 V or less in the pipette is divided by the pipette resistance and the series resistance of the cleft between the ...
James L, Rae, Richard A, Levis
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Using modified patch-clamp methodology, we demonstrated that it is possible to insert genes or other compounds routinely into single cells by electroporation. When the cell is indented by a small-tipped microelectrode, a voltage of 10 V or less in the pipette is divided by the pipette resistance and the series resistance of the cleft between the ...
James L, Rae, Richard A, Levis
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Electroporation of Physarum polycephalum
2003The protist Physarum polycephalum is a convenient system for studies of molecular and cellular biology of fundamental eukaryotic processes, including DNA replication, mitotic regulation, single-cell development, the cytoskeleton, and motility. The life cycle of this acellular slime mold exhibits a variety of developmental transitions, and the two ...
Burland, T. G., Bailey, Juliet
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Cell death due to electroporation – A review
Bioelectrochemistry, 2021Tina Batista Napotnik +1 more
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