Expression of Green Fluorescence Protein (GFP) in Zebrafish Muscle through Injection: A Gene Therapy Model [PDF]
, 2008 Expression of the target gene is important for gene therapy. Presently, localized transgenesis is used for gene therapy which can be achieved by a target gene expression. Here, we have reported the plasmid mediated gene therapy to zebrafish model.
C. H. Hsu +4 more
core +2 more sources
Kinetic Modeling and Numerical Simulation as Tools to Scale Microalgae Cell Membrane Permeabilization by Means of Pulsed Electric Fields (PEF) From Lab to Pilot Plants [PDF]
, 2020 Pulsed Electric Fields (PEF) is a promising technology for the gentle and energy efficient disruption of microalgae cells such as Chlorella vulgaris.
Knappert, Justus +2 more
core +1 more source
Non-viral delivery and optimized optogenetic stimulation of retinal ganglion cells led to behavioral restoration of vision [PDF]
, 2012 Stimulation of retinal neurons using optogenetics via use of chanelrhodopsin-2 (ChR2) has opened up a new direction for restoration of vision for treatment of retinitis pigmentosa (RP).
Edward Wong +5 more
core +2 more sources
BMC Biotechnology, 2023 Background Porcine fetal fibroblasts (PFFs) are important donor cells for generating genetically modified pigs, but the transfection efficiencies of PFFs are often unsatisfactory especially when large-size vectors are to be delivered.
Weiwei Liu +7 more
doaj +1 more source
Combined effect of neolamarckia cadamba leaves and electroporation method on hela cell anti- proliferation process [PDF]
, 2019 This study suggests that natural sources may become an important tool in treating cancer. Neolamarckia cadamba (NC) leaves also well-known as “Anthocephalus Cadamba”, is a precious plant in Ayurvedic medicine.
Abd Rahman, Nur Adilah +6 more
core +1 more source
Chemotactic properties of Escherichia coli mutants having abnormal Ca2+ content [PDF]
, 1995 The calA, calC, and calD mutants of Escherichia coli are known to be sensitive to Ca2+ (R. N. Brey and B. P. Rosen, J. Bacteriol. 139:824-834, 1979).
Adler, Julius, Tisa, Louis S.
core +3 more sources
Analysis of the Electric Field-Dependent Current During Electroporation Pulses
IEEE Access, 2020 When delivered across a cell, certain pulsed electric fields can cause an increase in the cell membrane permeability through a biophysical process known as electroporation.
Yanpeng Lv +6 more
doaj +1 more source
Molecules, 2021 Electroporation (EP) is one of the successful physical methods for intracellular drug delivery, which temporarily permeabilizes plasma membrane by exposing cells to electric pulses.
Tina Batista Napotnik +1 more
doaj +1 more source
Electroporation of Mycobacteria [PDF]
Journal of Visualized Experiments, 2008 High efficiency transformation is a major limitation in the study of mycobacteria. The genus Mycobacterium can be difficult to transform; this is mainly caused by the thick and waxy cell wall, but is compounded by the fact that most molecular techniques have been developed for distantly-related species such as Escherichia coli and Bacillus subtilis. In
Renan, Goude, Tanya, Parish
openaire +3 more sources
Gene doctoring: a method for recombineering in laboratory and pathogenic Escherichia coli strains [PDF]
, 2009 Background: Homologous recombination mediated by the lambda-Red genes is a common method for making chromosomal modifications in Escherichia coli.
Bingle, Lewis EH +6 more
core +5 more sources