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Mammalian Preimplantation Embryo Culture
, 2013 Since the inception of modern embryo culture media over 50 years ago there have been significant developments in culture systems for the mammalian preimplantation embryo. Carbohydrate gradients have been shown to impact embryo physiology and viability, while amino acids have been determined to have specific temporal effects during the preimplantation ...
Gardner, D.K., Lane, M.
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Symmetry Breaking in the Mammalian Embryo
Annual Review of Cell and Developmental Biology, 2018We present an overview of symmetry breaking in early mammalian development as a continuous process from compaction to specification of the body axes. While earlier studies have focused on individual symmetry-breaking events, recent advances enable us to explore progressive symmetry breaking during early mammalian development.
Takashi Hiiragi
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Cryopreservation of Mammalian Embryos
2007The cryopreservation of mammalian embryos has expanded over the past 20 yr by encompassing a range of sophisticated methods to deal with different developmental stages and different sensitivities to low-temperature exposure. We have described a method for slow, controlled-rate freezing of early stage embryos based on exposure to 1,2-propanediol and ...
Barry J, Fuller, Sharon J, Paynter
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The function of glypicans in the mammalian embryo
American Journal of Physiology-Cell Physiology, 2022Glypicans are proteoglycans that are bound to the outer surface of the plasma membrane by a glycosylphosphatidylinositol anchor. The mammalian genome contains six members of the glypican family ( GPC1 to GPC6). Although the degree of sequence homology within the family is rather low, the three-dimensional structure of these proteoglycans is highly ...
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Cryopreservation of mammalian embryos
Molecular Biotechnology, 1997As an innovative method for embryo cryopreservation, vitrification not only reduced the cooling stage duration to a minimum, but also eliminated any injuries cased by extracellular ice, which is a major cause of cell injury. Therefore, if embryos are treated adequately, high survival can be obtained.
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Calcium and neurulation in mammalian embryos
Development, 1985ABSTRACT The role of calcium in neurulation in rat embryos has been studied. Rat embryos at 10-4 days of gestation, when the cephalic neural folds have elevated but not fused, have been cultured in various media, and the effects of these media on the morphology of the cephalic neural folds have been observed by scanning and transmission ...
M J, Smedley, M, Stanisstreet
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MANIPULATION OF THE MAMMALIAN EMBRYO
Journal of Animal Science, 1985Technological advances in manipulation of mammalian embryos outside the maternal environment have resulted in opportunities for study of preimplantation embryo development, identification of developmental phenomena that are unique to mammals, and further improvement of technology.
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The Regulative Nature of Mammalian Embryos
2018The striking developmental plasticity of early mammalian embryos has been known since the classical experiments performed in the 1950s and 1960s. There are many lines of evidence that the mammalian embryo is able to continue normal development even when exposed to severe experimental manipulations of the number and position of cells within the embryo ...
Suwińska, Aneta +2 more
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DNA repair in mammalian embryos
Mutation Research/Reviews in Mutation Research, 2007Mammalian cells have developed complex mechanisms to identify DNA damage and activate the required response to maintain genome integrity. Those mechanisms include DNA damage detection, DNA repair, cell cycle arrest and apoptosis which operate together to protect the conceptus from DNA damage originating either in parental gametes or in the embryo's ...
Souraya, Jaroudi, Sioban, SenGupta
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Experimental genetics of the mammalian embryo
Journal of Cellular Physiology, 1982Recent progress in experimental mouse embryology has provided new approaches to the genetic manipulation of the mammalian embryo. The production of uniparental embryos enables one to compare maternal and paternal gene activity during development, to study the biological consequences of homozygosity of mutant genes, and to further elucidate the unsolved
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