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In vitro development of the mammalian embryo

Journal of Experimental Zoology, 1983
AbstractNormal growth and differentiation of mammalian embryos in vitro during the preimplantation period appear to be dependent upon the availability of appropriate metabolic substrates. For preimplantation embryos, defined conditions of culture have been achieved only in a few laboratory species.
M K, Sanyal, F, Naftolin
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Gene transfer into mammalian embryos

Human Reproduction, 1987
Transgenic mice provide a unique system fusing classical genetics with molecular biology. They enable the dissection of molecular mechanisms underlying the control of growth and differentiation, issues we only dreamed of 20 years ago. The transgenic technology can be expected to be applied also in areas like eugenetics of animal production systems as ...
P, Krimpenfort, A, Berns
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Nuclear Transfer in Mammalian Embryos

1990
Publisher Summary This chapter discusses the nuclear transfer in mammalian embryos. Transfer of nuclei from one cell to another provides a powerful method to study the interactions of the cytoplasm of one cell with the nuclei of another. Nuclei from various differentiated states can be transferred to nondifferentiated cytoplasm and the effect on the ...
R S, Prather, N L, First
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Erythropoiesis in the mammalian embryo

Experimental Hematology
Red blood cells (RBCs) comprise a critical component of the cardiovascular network, which constitutes the first functional organ system of the developing mammalian embryo. Examination of circulating blood cells in mammalian embryos revealed two distinct types of erythroid cells: large, nucleated "primitive" erythroblasts followed by smaller, enucleated
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Identification of Sex in Mammalian Embryos

1986
One of the uncertainties in reproduction of farm animals, regardless of whether one considers natural reproduction or reproduction assisted by artificial insemination or embryo transfer, is sex of the resulting off spring. This uncertainty can be of significant consequence when the economic value of one sex is considerably greater than that of the ...
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Cryopreservation of Mammalian Eggs and Embryos

1986
In the decade since reliable methods of freezing mouse embryos were first described (Whittingham et al., 1972; Wilmut, 1972a) methods have been developed for freezing the embryos of a number of other species. Frozen embryos have been used in the treatment of human infertility, in farm animal breeding, and in biological research. During this period, the
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Genetics of polarity in mammalian embryos

Reproductive BioMedicine Online, 2005
This brief review is devoted to the genetic control of polarity and embryonic axes in preimplantation mammalian embryos. Discussion is related to their formation, the considerable variations in gene activity in these early phases of development, and the influence of timers over polarities and related aspects of development.
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Apoptosis in Mammalian Embryos

2002
The preimplantation development of eutherian mammals is remarkably similar, spanning cleavage of the fertilized ovum; compaction and morula formation; and finally cavitation, with the formation of a blastocyst. Blastocyst formation marks the differentiation of the epithelial trophectoderm (TE), which is specialized for implantation, and the ...
Kate Hardy, Sophie Spanos
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Cryobiology: Preservation of Mammalian Embryos

1986
The preservation of mammalian embryos has become a routine procedure. Thousands of live offspring have been produced from frozen-thawed embryos transferred into recipient foster mothers. Species whose embryos have been successfully preserved include mouse, rat, rabbit, sheep, goat, cattle, horse, antelope, baboon, and human.
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Cryopreservation of Mammalian Embryos

1988
Early investigations of the effects of cooling and rewarming cells employed both spermatozoa and ova as experimental material. Spermatozoa were selected because of the ease of collection, inherent motility, and small size. Oocytes were employed mainly for their large size which allowed direct morphological observations.
Frank B. Kuzan, Patrick Quinn
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