Results 1 to 10 of about 10,608 (175)

YAP1 and QSER1 are key modulators of embryonic signaling pathways in the mammalian epiblast [PDF]

EMBO Reports
YAP1 signaling is essential for development but its specific roles in early embryogenesis remain poorly understood. To shed light on this, we analyze YAP1’s role in regulating the pluripotency of the mammalian epiblast, using scRNAseq approaches ...
Elizabeth Abraham, Thomas Roule, Aidan Douglas, Emily Megill, Olivia M Pericak, Jordan E Howe, Carmen Choya-Foces, Joanne F Garbincius, Henry M Cohen, Paula Roig-Flórez, Mikel Zubillaga, Mark D Andrake, Seonhee Kim, John W Elrod, Naiara Akizu, Conchi Estaras   +15 more
doaj   +2 more sources

Live imaging and functional characterization of the avian hypoblast redefine the mechanisms of primitive streak induction [PDF]

Nature Communications
In birds and mammals, the formation of the primitive streak, the hallmark of the primary axis and site of gastrulation, is thought to occur when the anterior displacement of the hypoblast (visceral endoderm in mice) lifts its inhibition on NODAL ...
Aurélien Villedieu, Olinda Alegria-Prévot, Carole Phan, Yu Ieda, Francis Corson, Jérôme Gros   +5 more
doaj   +2 more sources

The epigenetic modifier Fam208a is required to maintain epiblast cell fitness

Scientific Reports, 2017
Gastrulation initiates with the formation of the primitive streak, during which, cells of the epiblast delaminate to form the mesoderm and definitive endoderm.
Shohag Bhargava, Brian Cox, Christiana Polydorou, Veronika Gresakova, Vladimir Korinek, Hynek Strnad, Radislav Sedlacek, Trevor Allan Epp, Kallayanee Chawengsaksophak   +8 more
doaj   +1 more source

PDGFRα+ Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors

Stem Cell Reports, 2017
In early mouse pre-implantation development, primitive endoderm (PrE) precursors are platelet-derived growth factor receptor alpha (PDGFRα) positive. Here, we demonstrated that cultured mouse embryonic stem cells (mESCs) express PDGFRα heterogeneously ...
Antonio Lo Nigro, Anchel de Jaime-Soguero, Rita Khoueiry, Dong Seong Cho, Giorgia Maria Ferlazzo, Ilaria Perini, Vanesa Abon Escalona, Xabier Lopez Aranguren, Susana M. Chuva de Sousa Lopes, Kian Peng Koh, Pier Giulio Conaldi, Wei-Shou Hu, An Zwijsen, Frederic Lluis, Catherine M. Verfaillie   +14 more
doaj   +1 more source

Pluripotency Potential of Embryonic Stem Cell-Like Cells Derived from Mouse Testis

Cell Journal, 2019
Objective: During the cultivation of spermatogonial stem cells (SSCs) and their conversion into embryonic stem-like (ES-like) cells, transitional ES-like colonies and epiblast-like cells were observable.
Hossein Azizi, Behruz Asgari, Thomas Skutella   +2 more
doaj   +1 more source

The potent neuroepithelium-promoting activity of Otx2 during gastrulation, as demonstrated by its exogenous epiblast-wide expression in chicken embryos

Frontiers in Cell and Developmental Biology
Although Otx2 has been a topic of research for over 3 decades, the role of Otx2 expressed in the brain-forming anterior epiblast during gastrulation has not been clarified.
Naoya Takami, Kagayaki Kato, Koya Yoshihi, Akihito Kawamura, Hideaki Iida, Hisato Kondoh, Hisato Kondoh   +6 more
doaj   +1 more source

Single-Cell RNA-Seq Reveals Cellular Heterogeneity of Pluripotency Transition and X Chromosome Dynamics during Early Mouse Development

Cell Reports, 2019
Summary: Following implantation, the epiblast (EPI) cells transit from the naive to primed pluripotency, accompanied by dynamic changes in X chromosome activity in females. To investigate the molecular attributes of this process, we performed single-cell
Shangli Cheng, Yu Pei, Liqun He, Guangdun Peng, Björn Reinius, Patrick P.L. Tam, Naihe Jing, Qiaolin Deng   +7 more
doaj   +1 more source

p53-mediated regulation of epiblast cell numbers predicts reactivation during mouse embryonic diapause

Cell Reports
Summary: Embryonic diapause is a temporary suspension of proliferation in mammalian pre-implantation embryos, allowing for reactivation later. Cells in mouse diapause embryos enter the G0 phase within 7 days of diapause initiation.
Megumi Murata, Akihito Harada, Haruka Hirose, Hinako Maeda, Teppei Shimamura, Yasuyuki Ohkawa, Tatsuya Takemoto, Katsuyoshi Takaoka   +7 more
doaj   +1 more source

Primitive to visceral endoderm maturation is essential for mouse epiblast survival beyond implantation

iScience
Summary: The implantation of the mouse blastocyst initiates a complex sequence of tissue remodeling and cell differentiation events required for morphogenesis, during which the extraembryonic primitive endoderm transitions into the visceral endoderm ...
Antonia Weberling, Dylan Siriwardena, Christopher Penfold, Neophytos Christodoulou, Thorsten E. Boroviak, Magdalena Zernicka-Goetz   +5 more
doaj   +1 more source

Non-apical mitoses contribute to cell delamination during mouse gastrulation

Nature Communications
During the epithelial-mesenchymal transition driving mouse embryo gastrulation, cells divide more frequently at the primitive streak, and half of those divisions happen away from the apical pole.
Evangéline Despin-Guitard, Viviane S. Rosa, Steffen Plunder, Navrita Mathiah, Kristof Van Schoor, Eliana Nehme, Sara Merino-Aceituno, Joaquim Egea, Marta N. Shahbazi, Eric Theveneau, Isabelle Migeotte   +10 more
doaj   +1 more source