Results 1 to 10 of about 11,404 (119)

Re-biopsy and modified biopsy in mosaic human embryos with simple segmental chromosomal deletion [PDF]

open access: yesReproductive Biology and Endocrinology
Background There is a considerable proportion of false positive results when the result of preimplantation genetic testing for aneuploidy is chromosomal mosaicism.
Weizhou Wang   +7 more
doaj   +2 more sources

Revealing cell populations catching the early stages of human embryo development in naive pluripotent stem cell cultures

open access: yesStem Cell Reports, 2023
Summary: Naive human pluripotent stem cells (hPSCs) are defined as the in vitro counterpart of the human preimplantation embryo’s epiblast and are used as a model system to study developmental processes.
Marta Moya-Jódar   +13 more
doaj   +1 more source

A Shorter Equilibration Period Improves Post-Warming Outcomes after Vitrification and in Straw Dilution of In Vitro-Produced Bovine Embryos

open access: yesBiology, 2021
This study was designed to the optimize vitrification and in-straw warming protocol of in vitro-produced bovine embryos by comparing two different equilibration periods, short equilibrium (SE: 3 min) and long equilibrium (LE: 12 min).
Iris Martínez-Rodero   +10 more
doaj   +1 more source

microRNA expression during trophectoderm specification. [PDF]

open access: yesPLoS ONE, 2009
Segregation of the trophectoderm from the inner cell mass of the embryo represents the first cell-fate decision of mammalian development. Transcription factors essential for specifying trophectoderm have been identified, but the role of microRNAs (miRNAs)
Srinivas R Viswanathan   +5 more
doaj   +1 more source

The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey

open access: yesCells, 2021
Precise gene regulation is critical during embryo development. Long terminal repeat elements (LTRs) of endogenous retroviruses (ERVs) are dynamically expressed in blastocysts of mammalian embryos.
Kui Duan   +8 more
doaj   +1 more source

Effect of blastocyst morphology on the incidence of monozygotic twinning pregnancy after single blastocyst transfer

open access: yesGynecological Endocrinology, 2023
Aims: To explore whether blastocyst morphology [blastocyst stage, inner cell mass (ICM), and trophectoderm (TE) grading] impacts the occurrence of monozygotic twinning (MZT) after single blastocyst transfer (SBT).Materials and methods: A single-center ...
Xiaofei Ge   +3 more
doaj   +1 more source

Karyotype of the Blastocoel Fluid Derived by Laser-Assisted Hatching Demonstrates a Low Agreement With the Trophectoderm

open access: yesFrontiers in Physiology, 2022
ObjectiveThe aim of this study is to compare the amplification efficiency and the genomic profiles of blastocoel fluid (BF) derived by laser-assisted hatching and trophectoderm (TE) cells derived from the same blastocyst.MethodsFifty-four fresh ...
Liang Wang   +6 more
doaj   +1 more source

Development of an Improved in vitro Model of Bovine Trophectoderm Differentiation

open access: yesFrontiers in Animal Science, 2022
The mechanisms regulating early stages of placentation and trophectoderm differentiation in the ruminant conceptus remain poorly understood. Here we present a model of trophectoderm (TE) differentiation in vitro from outgrowths of individual in vitro ...
M. Sofia Ortega   +9 more
doaj   +1 more source

Cytoplasmic strings between ICM and mTE are a positive predictor of clinical pregnancy and live birth outcomes: A time-lapse study

open access: yesFrontiers in Medicine, 2022
BackgroundElective single blastocyst transfer (eSBT) is considered to reduce the incidence of multiple pregnancy compared to double embryo transfer. Blastocyst selection is the key to achieving pregnancy.
Bing-Xin Ma   +4 more
doaj   +1 more source

High-resolution transcriptional and morphogenetic profiling of cells from micropatterned human ESC gastruloid cultures

open access: yeseLife, 2020
During mammalian gastrulation, germ layers arise and are shaped into the body plan while extraembryonic layers sustain the embryo. Human embryonic stem cells, cultured with BMP4 on extracellular matrix micro-discs, reproducibly differentiate into ...
Kyaw Thu Minn   +7 more
doaj   +1 more source

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