Results 251 to 260 of about 212,225 (290)

Cryopreservation of Mammalian Embryos

2007
The cryopreservation of mammalian embryos has expanded over the past 20 yr by encompassing a range of sophisticated methods to deal with different developmental stages and different sensitivities to low-temperature exposure. We have described a method for slow, controlled-rate freezing of early stage embryos based on exposure to 1,2-propanediol and ...
Barry J, Fuller, Sharon J, Paynter
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Gene transfer into mammalian embryos

Human Reproduction, 1987
Transgenic mice provide a unique system fusing classical genetics with molecular biology. They enable the dissection of molecular mechanisms underlying the control of growth and differentiation, issues we only dreamed of 20 years ago. The transgenic technology can be expected to be applied also in areas like eugenetics of animal production systems as ...
P, Krimpenfort, A, Berns
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Mammalian Preimplantation Embryo Culture

2013
Since the inception of modern embryo culture media over 50 years ago there have been significant developments in culture systems for the mammalian preimplantation embryo. Carbohydrate gradients have been shown to impact embryo physiology and viability, while amino acids have been determined to have specific temporal effects during the preimplantation ...
Gardner, D.K., Lane, M.
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Cryopreservation of mammalian embryos

Molecular Biotechnology, 1997
As an innovative method for embryo cryopreservation, vitrification not only reduced the cooling stage duration to a minimum, but also eliminated any injuries cased by extracellular ice, which is a major cause of cell injury. Therefore, if embryos are treated adequately, high survival can be obtained.
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DNA repair in mammalian embryos

Mutation Research/Reviews in Mutation Research, 2007
Mammalian cells have developed complex mechanisms to identify DNA damage and activate the required response to maintain genome integrity. Those mechanisms include DNA damage detection, DNA repair, cell cycle arrest and apoptosis which operate together to protect the conceptus from DNA damage originating either in parental gametes or in the embryo's ...
Souraya, Jaroudi, Sioban, SenGupta
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Nuclear Transfer in Mammalian Embryos

1990
Publisher Summary This chapter discusses the nuclear transfer in mammalian embryos. Transfer of nuclei from one cell to another provides a powerful method to study the interactions of the cytoplasm of one cell with the nuclei of another. Nuclei from various differentiated states can be transferred to nondifferentiated cytoplasm and the effect on the ...
R S, Prather, N L, First
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MANIPULATION OF THE MAMMALIAN EMBRYO

Journal of Animal Science, 1985
Technological advances in manipulation of mammalian embryos outside the maternal environment have resulted in opportunities for study of preimplantation embryo development, identification of developmental phenomena that are unique to mammals, and further improvement of technology.
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Experimental genetics of the mammalian embryo

Journal of Cellular Physiology, 1982
Recent progress in experimental mouse embryology has provided new approaches to the genetic manipulation of the mammalian embryo. The production of uniparental embryos enables one to compare maternal and paternal gene activity during development, to study the biological consequences of homozygosity of mutant genes, and to further elucidate the unsolved
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Postimplantation mammalian embryos

1993
Abstract Techniques for culturing whole postimplantation mammalian embryos were pioneered by D.A.T. New in Cambridge (1, 2). Originally, rat embryos were cultured on plasma clots in watch glasses. Better development was found to result from the use of blood serum as a culture medium and by the use of ‘circulators’, in which ...
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Postiplantation Mammalian Embryos

1990
Abstract In recent years, the quest for greater understanding of postimplantation mammalian development has led research workers to devise techniques which overcome the problems of inaccessibility posed by embryos in the uterus, and to adapt analytic techniques such as in situ hybridization and antibody labelling to the unique ...
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