Results 251 to 260 of about 206,297 (286)

Cryopreservation of Mammalian Embryos

2007
The cryopreservation of mammalian embryos has expanded over the past 20 yr by encompassing a range of sophisticated methods to deal with different developmental stages and different sensitivities to low-temperature exposure. We have described a method for slow, controlled-rate freezing of early stage embryos based on exposure to 1,2-propanediol and ...
S.J. Paynter, Barry Fuller
openaire   +3 more sources

Gene transfer into mammalian embryos

Human Reproduction, 1987
Transgenic mice provide a unique system fusing classical genetics with molecular biology. They enable the dissection of molecular mechanisms underlying the control of growth and differentiation, issues we only dreamed of 20 years ago. The transgenic technology can be expected to be applied also in areas like eugenetics of animal production systems as ...
Paul Krimpenfort, Anton Berns
openaire   +3 more sources

In vitro development of the mammalian embryo

Journal of Experimental Zoology, 1983
AbstractNormal growth and differentiation of mammalian embryos in vitro during the preimplantation period appear to be dependent upon the availability of appropriate metabolic substrates. For preimplantation embryos, defined conditions of culture have been achieved only in a few laboratory species.
Mrinal K. Sanyal, Frederick Naftolin
openaire   +3 more sources

Mammalian Preimplantation Embryo Culture

2013
Since the inception of modern embryo culture media over 50 years ago there have been significant developments in culture systems for the mammalian preimplantation embryo. Carbohydrate gradients have been shown to impact embryo physiology and viability, while amino acids have been determined to have specific temporal effects during the preimplantation ...
Gardner, D.K., Lane, M.
openaire   +4 more sources

Calcium and neurulation in mammalian embryos

Development, 1985
ABSTRACT The role of calcium in neurulation in rat embryos has been studied. Rat embryos at 10-4 days of gestation, when the cephalic neural folds have elevated but not fused, have been cultured in various media, and the effects of these media on the morphology of the cephalic neural folds have been observed by scanning and transmission ...
Martin Stanisstreet, Martin J. Smedley
openaire   +3 more sources

MANIPULATION OF THE MAMMALIAN EMBRYO

Journal of Animal Science, 1985
Technological advances in manipulation of mammalian embryos outside the maternal environment have resulted in opportunities for study of preimplantation embryo development, identification of developmental phenomena that are unique to mammals, and further improvement of technology.
openaire   +2 more sources

Experimental genetics of the mammalian embryo

Journal of Cellular Physiology, 1982
Recent progress in experimental mouse embryology has provided new approaches to the genetic manipulation of the mammalian embryo. The production of uniparental embryos enables one to compare maternal and paternal gene activity during development, to study the biological consequences of homozygosity of mutant genes, and to further elucidate the unsolved
openaire   +3 more sources

Apoptosis in Mammalian Embryos

2002
The preimplantation development of eutherian mammals is remarkably similar, spanning cleavage of the fertilized ovum; compaction and morula formation; and finally cavitation, with the formation of a blastocyst. Blastocyst formation marks the differentiation of the epithelial trophectoderm (TE), which is specialized for implantation, and the ...
S Spanos, Kate Hardy
openaire   +2 more sources

Cryopreservation of Mammalian Embryos

1988
Early investigations of the effects of cooling and rewarming cells employed both spermatozoa and ova as experimental material. Spermatozoa were selected because of the ease of collection, inherent motility, and small size. Oocytes were employed mainly for their large size which allowed direct morphological observations.
Patrick Quinn, Frank B. Kuzan
openaire   +2 more sources

The function of glypicans in the mammalian embryo

American Journal of Physiology-Cell Physiology, 2022
Glypicans are proteoglycans that are bound to the outer surface of the plasma membrane by a glycosylphosphatidylinositol anchor. The mammalian genome contains six members of the glypican family ( GPC1 to GPC6). Although the degree of sequence homology within the family is rather low, the three-dimensional structure of these proteoglycans is highly ...
openaire   +2 more sources