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Gene transfer into mammalian embryos

Human Reproduction, 1987
Transgenic mice provide a unique system fusing classical genetics with molecular biology. They enable the dissection of molecular mechanisms underlying the control of growth and differentiation, issues we only dreamed of 20 years ago. The transgenic technology can be expected to be applied also in areas like eugenetics of animal production systems as ...
P, Krimpenfort, A, Berns
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Cryopreservation of mammalian embryos

Molecular Biotechnology, 1997
As an innovative method for embryo cryopreservation, vitrification not only reduced the cooling stage duration to a minimum, but also eliminated any injuries cased by extracellular ice, which is a major cause of cell injury. Therefore, if embryos are treated adequately, high survival can be obtained.
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MANIPULATION OF THE MAMMALIAN EMBRYO

Journal of Animal Science, 1985
Technological advances in manipulation of mammalian embryos outside the maternal environment have resulted in opportunities for study of preimplantation embryo development, identification of developmental phenomena that are unique to mammals, and further improvement of technology.
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Calcium and neurulation in mammalian embryos

Development, 1985
ABSTRACT The role of calcium in neurulation in rat embryos has been studied. Rat embryos at 10-4 days of gestation, when the cephalic neural folds have elevated but not fused, have been cultured in various media, and the effects of these media on the morphology of the cephalic neural folds have been observed by scanning and transmission ...
M J, Smedley, M, Stanisstreet
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Experimental genetics of the mammalian embryo

Journal of Cellular Physiology, 1982
Recent progress in experimental mouse embryology has provided new approaches to the genetic manipulation of the mammalian embryo. The production of uniparental embryos enables one to compare maternal and paternal gene activity during development, to study the biological consequences of homozygosity of mutant genes, and to further elucidate the unsolved
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The Regulative Nature of Mammalian Embryos

2018
The striking developmental plasticity of early mammalian embryos has been known since the classical experiments performed in the 1950s and 1960s. There are many lines of evidence that the mammalian embryo is able to continue normal development even when exposed to severe experimental manipulations of the number and position of cells within the embryo ...
Katarzyna, Klimczewska   +2 more
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Mammalian Preimplantation Embryo Culture

2013
Since the inception of modern embryo culture media over 50 years ago there have been significant developments in culture systems for the mammalian preimplantation embryo. Carbohydrate gradients have been shown to impact embryo physiology and viability, while amino acids have been determined to have specific temporal effects during the preimplantation ...
Gardner, D.K., Lane, M.
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DNA repair in mammalian embryos

Mutation Research/Reviews in Mutation Research, 2007
Mammalian cells have developed complex mechanisms to identify DNA damage and activate the required response to maintain genome integrity. Those mechanisms include DNA damage detection, DNA repair, cell cycle arrest and apoptosis which operate together to protect the conceptus from DNA damage originating either in parental gametes or in the embryo's ...
Souraya, Jaroudi, Sioban, SenGupta
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Symmetry Breaking in the Mammalian Embryo

Annual Review of Cell and Developmental Biology, 2018
We present an overview of symmetry breaking in early mammalian development as a continuous process from compaction to specification of the body axes. While earlier studies have focused on individual symmetry-breaking events, recent advances enable us to explore progressive symmetry breaking during early mammalian development.
Hui Ting, Zhang, Takashi, Hiiragi
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Nuclear Transfer in Mammalian Embryos

1990
Publisher Summary This chapter discusses the nuclear transfer in mammalian embryos. Transfer of nuclei from one cell to another provides a powerful method to study the interactions of the cytoplasm of one cell with the nuclei of another. Nuclei from various differentiated states can be transferred to nondifferentiated cytoplasm and the effect on the ...
R S, Prather, N L, First
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