Results 21 to 30 of about 536,166 (300)

Optical transfection and injection techniques applied to mammalian and embryonic cells [PDF]

, 2012
The delivery of biomolecules into living cells is an important methodology in cell and molecular biology. Optical methods using lasers are attractive tools for such application.
Torres, Maria Leilani
core   +2 more sources

Actomyosin polarisation through PLC-PKC triggers symmetry breaking of the mouse embryo

Nature Communications, 2017
The molecular trigger that establishes cell polarity in the mammalian embryo is unclear. Here, the authors show that de novo polarisation of the mouse embryo at the 8-cell stage is directed by Phospholipase C and Protein kinase C and occurs in two phases:
Meng Zhu, Chuen Yan Leung, Marta N. Shahbazi, Magdalena Zernicka-Goetz   +3 more
doaj   +1 more source

Oocyte specific lncRNA variant Rose influences oocyte and embryo development

Non-coding RNA Research, 2021
Fully grown mammalian oocytes store a large amount of RNA synthesized during the transcriptionally active growth stage. A large part of the stored RNA belongs to the long non-coding class which contain either transcriptional noise or important ...
Rajan Iyyappan, Daria Aleshkina, Linkai Zhu, Zongliang Jiang, Veronika Kinterova, Andrej Susor   +5 more
doaj   +1 more source

Morphogenesis of extra-embryonic tissues directs the remodelling of the mouse embryo at implantation

Nature Communications, 2019
How the shape of the pre-implantation murine embryo changes dramatically upon implantation is unclear. Here, the authors use live imaging with a cdx2-GFP reporter line in combination with loss of function experiments to demonstrate that FGF signalling ...
Neophytos Christodoulou, Antonia Weberling, Douglas Strathdee, Kurt I. Anderson, Paul Timpson, Magdalena Zernicka-Goetz   +5 more
doaj   +1 more source

Cell arrest and cell death in mammalian preimplantation development [PDF]

, 2011
The causes, modes, biological role and prospective significance of cell death in preimplantation development in humans and other mammals are still poorly understood.
Tuna Güngör, Eckhard Wolf, Güngör Tuna, Horst-Dieter Reichenbach, Leidenfrost, Sandra, Reichenbach, Myriam, Reichenbach Myriam, Leidenfrost Sandra, Reichenbach, Horst-Dieter, Myriam Reichenbach, Sandra Leidenfrost, Marc Boelhauve, Fred Sinowatz, Habermann, Felix A., Felix A Habermann, Wolf Eckhard, Felix A. Habermann, Boelhauve, Marc, Güngör, Tuna, Boelhauve Marc, Wolf, Eckhard, Sinowatz, Fred, Reichenbach Horst-Dieter, Sinowatz Fred, Habermann Felix A.   +24 more
core   +2 more sources

Biosynthesis and expression of zona pellucida glycoproteins in mammals [PDF]

, 2001
The zona pellucida (ZP) is an extracellular matrix surrounding the oocyte and the early embryo that exerts several important functions during fertilization and early embryonic development.
Töpfer-Petersen, E., Kölle, S., Sinowatz, Fred   +2 more
core   +1 more source

Insights into epigenetic patterns in mammalian early embryos

Protein & Cell, 2020
Mammalian fertilization begins with the fusion of two specialized gametes, followed by major epigenetic remodeling leading to the formation of a totipotent embryo. During the development of the pre-implantation embryo, precise reprogramming progress is a
Ruimin Xu, Chong Li, Xiaoyu Liu, Shaorong Gao   +3 more
doaj   +1 more source

Endometrial stromal cells of women with recurrent miscarriage fail to discriminate between high- and low-quality human embryos [PDF]

, 2012
Background The aetiology of recurrent miscarriage (RM) remains largely unexplained. Women with RM have a shorter time to pregnancy interval than normally fertile women, which may be due to more frequent implantation of non-viable embryos.
Weimar, Charlotte H. E., Gellersen, Birgit, Heijnen Cobi J., Johanna M T de Vreeden-Elbertse, Jan J. Brosens (109247), de Vreeden-Elbertse Johanna M. T., de Vreeden-Elbertse, Johanna M.T., Nick S Macklon, Macklon Nick S., Kavelaars, Annemieke, Charlotte H. E. Weimar (148304), Nick S. Macklon (148320), Jan J Brosens, Weimar Charlotte H. E., Gellersen Birgit, Cobi J. Heijnen (148314), Brosens Jan J., Macklon, Nick S., Weimar, Charlotte H.E., Kavelaars Annemieke, Cobi J Heijnen, Charlotte H E Weimar, Birgit Gellersen (148307), de Vreeden-Elbertse, Johanna M. T., Brosens, Jan J., Heijnen, Cobi J., Annemieke Kavelaars, Annemieke Kavelaars (12570), Birgit Gellersen, Johanna M. T. de Vreeden-Elbertse (148309)   +29 more
core   +2 more sources

Embryo donation parents' attitudes towards donors : comparison with adoption [PDF]

, 2008
BACKGROUND: Embryo donation produces a family structure where neither rearing parent is genetically related to the child, as in adoption. It is not known how embryo donation parents view the donors compared with how adoptive parents view the birth ...
MacCallum, Fiona
core   +1 more source

The meganuclease I-SceI containing nuclear localization signal (NLS-I-SceI) efficiently mediated mammalian germline transgenesis via embryo cytoplasmic microinjection. [PDF]

PLoS ONE, 2014
The meganuclease I-SceI has been effectively used to facilitate transgenesis in fish eggs for nearly a decade. I-SceI-mediated transgenesis is simply via embryo cytoplasmic microinjection and only involves plasmid vectors containing I-SceI recognition ...
Yong Wang, Xiao-Yang Zhou, Peng-Ying Xiang, Lu-Lu Wang, Huan Tang, Fei Xie, Liang Li, Hong Wei   +7 more
doaj   +1 more source