Results 71 to 80 of about 1,293,017 (382)

Improving single-cell cloning workflow for gene editing in human pluripotent stem cells [PDF]

, 2018
The availability of human pluripotent stem cells (hPSCs) and progress in genome engineering technology have altered the way we approach scientific research and drug development screens.
Chen, Yi-Hsien, Pruett-Miller, Shondra M
core   +2 more sources

Improvement in XIa Selectivity of Snake Venom Peptide Analogue BF9-N17K Using P2′ Amino Acid Replacements

Toxins
Coagulation factor XIa is a new serine-protease family drug target for next-generation anticoagulants. With the snake venom Kunitz-type peptide BF9 as the scaffold, we obtained a highly active XIa inhibitor BF9-N17K in our previous work, but it also ...
Li Ding, Zhiping Zhai, Tianxiang Qin, Yuexi Lin, Zhicheng Shuang, Fang Sun, Chenhu Qin, Hongyi Luo, Wen Zhu, Xiangdong Ye, Zongyun Chen, Xudong Luo   +11 more
doaj   +1 more source

Mouse embryonic stem cells self-organize into trunk-like structures with neural tube and somites

Science, 2020
Trunk formation in a dish Building mammalian embryos from self-organizing stem cells in culture would accelerate the investigation of morphogenetic and differentiation processes that shape the body plan. Veenvliet et al.
Jesse V. Veenvliet, Adriano Bolondi, H. Kretzmer, Leah Haut, Manuela Scholze-Wittler, Dennis Schifferl, Frederic Koch, L. Guignard, Abhishek Sampath Kumar, Milena Pustet, Simon Heimann, René Buschow, L. Wittler, B. Timmermann, A. Meissner, B. Herrmann   +15 more
semanticscholar   +1 more source

Soman induces endoplasmic reticulum stress and apoptosis of cerebral organoids via the GRP78‐ATF6‐CHOP signaling pathway

FEBS Open Bio, EarlyView.
Cerebral organoids were employed as a novel model to explore the neurotoxicity of soman. Soman inhibited acetylcholinesterase activity, increased cell apoptosis and upregulated endoplasmic reticulum (ER) stress markers glucose‐regulated protein 78 (GRP78), activating transcription factor 6 (ATF6) and C/EBP homologous protein (CHOP).
Yue Wei, Zhanbiao Liu, Jingjing Shi, Qian Jin, Wenqian Chen, Xuejun Chen, Liqin Li, Hui Chen   +7 more
wiley   +1 more source

Human Embryonic Stem Cell-Derived Oligodendrocyte Progenitor Cell Transplants Remyelinate and Restore Locomotion after Spinal Cord Injury

Journal of Neuroscience, 2005
Demyelination contributes to loss of function after spinal cord injury, and thus a potential therapeutic strategy involves replacing myelin-forming cells.
H. Keirstead, G. Nistor, G. Bernal, Minodora O. Totoiu, Frank Cloutier, K. Sharp, O. Steward   +6 more
semanticscholar   +1 more source

Co‐expression of HSV‐1 ICP34.5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity

FEBS Open Bio, EarlyView.
ICP34.5 is one of the most important antihost response proteins. The saRNA‐encoding HSV‐1 neurovirulence protein ICP34.5 clearly mediated the eukaryotic initiation factor 2 alpha subunit (eIF2α) dephosphorylation and significant suppression of innate immune responses in vitro, leading to enhanced expression of the saRNA‐encoded gene.
Xuemin Lu, Yabin Wu, Chunye Zhao, Jie Zheng, Shangwu Chen, Yigang Wang, Yulong Xia   +6 more
wiley   +1 more source

Stem Cell Therapeutics: Exploring Newer Alternatives to Human Embryonic Stem Cells [PDF]

, 2008
Stem cells therapeutics has come a long way since stem cells and their potential was discovered for the first time. Intense research into cellular biology of stem cells has revealed that they possess immense potential for curing many human diseases ...
Garg, Abhishek D.
core  

Exclusive multipotency and preferential asymmetric divisions in post-embryonic neural stem cells of the fish retina. [PDF]

, 2014
The potency of post-embryonic stem cells can only be addressed in the living organism, by labeling single cells after embryonic development and following their descendants.
Ander, Janina-J, Centanin, Lázaro, Harris, William A, Hasel, Eva, Hoeckendorf, Burkhard, Kellner, Tanja, Kraemer, Isabel, Lust, Katharina, Simons, Benjamin D, Urbany, Cedric, Wittbrodt, Joachim   +10 more
core   +1 more source

Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines

Stem Cells International, 2016
Although human pluripotent stem cells (hPSCs) can proliferate robustly on the feeder-free culture system, genetic instability of hPSCs has been reported in such environment. Alternatively, feeder cells enable hPSCs to maintain their pluripotency.
Ruttachuk Rungsiwiwut, Praewphan Ingrungruanglert, Pranee Numchaisrika, Pramuan Virutamasen, Tatsanee Phermthai, Kamthorn Pruksananonda   +5 more
doaj   +1 more source

Immunosuppression by Embryonic Stem Cells [PDF]

Stem Cells, 2007
AbstractEmbryonic stem cells or their progeny inevitably differ genetically from those who might receive the cells as transplants. We tested the barriers to engraftment of embryonic stem cells and the mechanisms that determine those barriers. Using formation of teratomas as a measure of engraftment, we found that semiallogeneic and fully allogeneic ...
Cody A. Koch, Jeffrey L. Platt, Pedro Geraldes   +2 more
openaire   +3 more sources