To cleave or not and how? The DNA exonucleases and endonucleases in immunity
Genes and DiseasesDNA exonucleases and endonucleases are key executors of the genome during many physiological processes. They generate double-stranded DNA by cleaving damaged endogenous or exogenous DNA, triggering the activation of the innate immune pathways such as ...
Mingjun Lu +5 more
doaj +1 more source
Nucleic Acids Research, 2012 The ability to specifically engineer the genome of living cells at precise locations using rare-cutting designer endonucleases has broad implications for biotechnology and medicine, particularly for functional genomics, transgenics and gene therapy ...
F. Daboussi +30 more
semanticscholar +1 more source
Advanced Science, EarlyView.We analyzed long‐read genomic sequencing data obtained from 40 inbred mouse strains to produce a large database of structural variants. This dataset captures the major types of structural variants, which includes deletions, insertions, duplications, and inversions.
Wenlong Ren +6 more
wiley +1 more source
The role of DNA restriction-modification systems in the biology of Bacillus anthracis
Frontiers in Microbiology, 2016 Restriction-modification (R-M) systems are widespread among prokaryotes and, depending on their type, may be viewed as selfish genetic elements that persist as toxin-antitoxin modules or as cellular defense systems against phage infection.
Ramakrishnan eSitaraman
doaj +1 more source
The Human Pregnancy-Specific Glycoprotein Genes are Tightly Linked on the Long Arm of Chromosome 19 and are Coordinately Expressed [PDF]
, 1990 The pregnancy-specific glycoprotein (PSG) genes encode a group of proteins which are found in large amounts in placenta and maternal serum. In situ hybridization analyses of metaphase chromosomes reveal that all the human pregnancy-specific glycoprotein (
Barnett +40 more
core +1 more source
Distinct roles of structure-specific endonucleases EEPD1 and Metnase in replication stress responses [PDF]
, 2020 Neelam Sharma +7 more
openalex +1 more source
Engineering RNA Endonucleases with Customized Sequence Specificities
Nature Communications, 2012 Specific cleavage of RNAs is critical for in vitro manipulation of RNA and for in vivo gene silencing. Here we engineer artificial site-specific RNA endonucleases to function analogously to DNA restriction enzymes.
Rajarshi Choudhury +4 more
semanticscholar +1 more source
Generating golden Syrian hamsters with conditional alleles via zygote microinjection of CRISPR/Cas9
Animal Models and Experimental Medicine, EarlyView.We established the first conditional knockout (cKO) model in the golden Syrian hamster by CRISPR/Cas9‐mediated genome editing. Cas9 protein, two sgRNAs, and a donor plasmid carrying loxP‐flanked exon 2 of the ApoF gene were microinjected into one‐cell embryos. The floxed allele was efficiently generated (up to 27%) and transmitted through the germline.
Wei Chen +16 more
wiley +1 more source
Expression of Gene Product 126 from Phage Gizmo and Creation of a Substrate [PDF]
, 2014 Endonucleases are enzymes which cleave DNA at specific nucleotide sequences. Homing Endonucleases (HEase) are a group of endonucleases that reside as open reading frames within self-splicing introns or inteins. After expression of the gene containing the
Bollivar, Faculty Advisor, David +1 more
core +1 more source
Plant-RRBS, a bisulfite and next-generation sequencing-based methylome profiling method enriching for coverage of cytosine positions [PDF]
, 2017 Background: Cytosine methylation in plant genomes is important for the regulation of gene transcription and transposon activity. Genome-wide methylomes are studied upon mutation of the DNA methyltransferases, adaptation to environmental stresses or ...
A Akalin +60 more
core +3 more sources