Results 101 to 110 of about 60,825 (304)

Selective microbial genomic DNA isolation using restriction endonucleases.

PLoS ONE, 2014
To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads.
Helen E Barnes, Guohong Liu, Christopher Q Weston, Paula King, Long K Pham, Shannon Waltz, Kimberly T Helzer, Laura Day, Dan Sphar, Robert T Yamamoto, R Allyn Forsyth   +10 more
doaj   +1 more source

Isolation, characterization and molecular cloning of restriction endonucleases.

, 1990
by Leung Sau-mei.With: Two articles bound together subsequent to publication.Thesis (M.Phil.)--Chinese University of Hong Kong, 1990.Bibliography: leaves 101-108.Abstract --- p.iAcknowledgements --- p.ivList of Abbreviations --- p.vTable of content ---

core  

MOESM1 of Identification and characterization of DNA endonucleases in Plasmodium falciparum 3D7 clone

, 2018
Additional file 1. Phylogenetic analysis of seven P. falciparum 3D7 DNA endonucleases with homologous proteins of other species. Amino acid sequences were aligned using MEGA 6, and a phylogenetic tree was generated by the neighbour-joining method.
Yiwei Zhang (1448599), Yiwei Zhang, Jianping Li (300447), Zhiwei Tu, Qijun Chen (21638), Jianping Li, Ying Feng, Na Yang (266025), Na Yang, Ning Jiang, Zhiwei Tu (5413235), Xiaoyu Sang (5232929), Xiaoyu Sang, Ning Jiang (118848), Ying Feng (151159), Qijun Chen   +15 more
core   +1 more source

Commonalities of platelet dysfunction in heart failure with preserved ejection fraction and underlying comorbidities

ESC Heart Failure, Volume 12, Issue 2, Page 1013-1028, April 2025.
Abstract Heart failure with preserved ejection fraction (HFpEF) is characterized by a lack of a specific targeted treatment and a complex, partially unexplored pathophysiology. Common comorbidities associated with HFpEF are hypertension, atrial fibrillation, obesity and diabetes.
Giorgia D'Italia, Blanche Schroen, Judith M.E.M. Cosemans   +2 more
wiley   +1 more source

Effect of endonucleases inhibitor in mice sperm mediated gene transfer

, 2012
A baixa eficiência e a dificuldade de reprodução de resultados da técnica de transferência gênica mediada por espermatozoides (TGME) têm como possível explicação à ativação de endonucleases espermáticas.
Fernanda Sevciuc Maria, Maria, Fernanda Sevciuc   +1 more
core   +1 more source

Bridging the Gap Between Human Toxicology and Ecotoxicology Under One Health Perspective by a Cross‐Species Adverse Outcome Pathway Network for Reproductive Toxicity

Environmental Toxicology and Chemistry, EarlyView.
Cross‐species extrapolation of adverse outcome pathway network on reproductive toxicity under the One Health perspective using new approach methodologies. AOP = adverse outcome pathway. Abstract Although ecotoxicological and toxicological risk assessments are performed separately from each other, recent efforts have been made in both disciplines to ...
Elizabeth Dufourcq Sekatcheff, Jaeseong Jeong, Jinhee Choi   +2 more
wiley   +1 more source

Homing endonucleases: from basics to therapeutic applications

Homing endonucleases (HE) are double-stranded DNAses that target large recognition sites (12-40 bp). HE-encoding sequences are usually embedded in either introns or inteins.
Montoya, Guillermo, Blanco, Francisco J, Marcaida, Maria J, Prieto, Jesús, Muñoz, Inés G   +4 more
core   +1 more source

Silencing SGO2 by Oxamic Acid Dissociates Glycolysis and BRCA1‐Mediated DNA Repair to Improve the Chemosensitivity of Lung Adenocarcinoma

Exploration, EarlyView.
SGO2 interacts with BRCA1 to inhibit BRCA1 ubiquitination and degradation, thereby promoting BRCA1‐induced DNA damage repair signaling and reducing the chemo sensitivity of LUADa. OA targets glycolysis to disrupt H3K18la‐ and H3K27ac‐mediated chromatin accessibility, repressing SGO2 transcription and subsequently alleviating SGO2‐mediated cancer ...
Xian Lin, Zhidan Hua, Chen Liu, Minxia Yang, Beilei Zhang, Xiaofeng Zhu, Xiao Chen   +6 more
wiley   +1 more source

Neurolathyrism in Sub‐Saharan Africa—Assessing the Neurotoxic Risks of Lathyrus sativus Amid Drought and Food Security Challenges

Food Safety and Health, EarlyView.
Representation of grass pea consumption in drought‐stricken sub‐Saharan Africa sustains nutrition, but excess β‐ODAP exposure due to multiple reasons triggers neurolathyrism, a progressive neurotoxic disorder. ABSTRACT Neurolathyrism is a progressive motor neuron disease due to the consumption of Lathyrus sativus (grass pea) over long periods.
Biruk Demisse Ayalew, Muhammad Umar, Pakeezah Tabasum, Maria Qadri, Talha Farooq, Eskeatnaf Yosef Getachew, Zemichael Getu Alemayehu, Eden Haile Hagos, Hailemariam Shimelis Gebeyehu, Hanna Tsehay Abebe, Nuradin Abdi Ali, David Chinaecherem Innocent, Temesgen Mamo Sharew   +12 more
wiley   +1 more source

Three new active members of the I-OnuI family of homing endonucleases

, 2017
In vitro characterization of three LAGLIDADG-type homing endonucleases (I-CcaI, I-CcaII and I-AstI) that belong to the I-OnuI family showed that they are functional homing endonucleases that cleave their respective cognate target sites.
Hausner, Georg, Bilto, Iman M., Alvan Wai, Wai, Alvan, Guha , Tuhin Kumar, Tuhin K. Guha, Iman M. Bilto, Georg Hausner   +7 more
core   +1 more source