Results 101 to 110 of about 64,056 (293)
Genome editing using programmable endonucleases
, 2014 Programmable endonucleases are engineered proteins that recognize specific nucleotide sequences and that are capable of introducing double-strand breaks within these sequences. Zinc-finger nucleases have been used extensively as a tool in genome editing,
Hanečková, Radmila
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Selective microbial genomic DNA isolation using restriction endonucleases.
PLoS ONE, 2014 To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads.
Helen E Barnes +10 more
doaj +1 more source
Isolation, characterization and molecular cloning of restriction endonucleases.
, 1990 by Leung Sau-mei.With: Two articles bound together subsequent to publication.Thesis (M.Phil.)--Chinese University of Hong Kong, 1990.Bibliography: leaves 101-108.Abstract --- p.iAcknowledgements --- p.ivList of Abbreviations --- p.vTable of content ---
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Homing endonucleases: from basics to therapeutic applications
Homing endonucleases (HE) are double-stranded DNAses that target large recognition sites (12-40 bp). HE-encoding sequences are usually embedded in either introns or inteins.
Montoya, Guillermo +4 more
core +1 more source
Cleavage and sequence recognition of 2,6-diaminopurine-containing DNA by site-specific endonucleases [PDF]
, 1987 The susceptibility of 2,6-diaminopurine (DAP)-containing bacteriophage DNA to several restriction and other endonucleases was examined. With the only exception of TaqI, these enzymes did not accept the modified base as a substitute for adenine. The phage
Andrey V. Matveyev +3 more
core +1 more source
Chicken Pulmonary MicroRNAs Targeting the PB2 (Segment 1) of Avian Influenza Virus
Animal Research and One Health, EarlyView.The PB2 segment of H5N1 is essential for replication and host adaptation. We screened 200 miRNAs and identified five (gga‐miR‐17‐3p, gga‐miR‐29a‐5p, gga‐miR‐1718, gga‐miR‐16c‐5p, and gga‐miR‐1744‐5p) using thermodynamic stability of heteroduplex, seed sequence complementarity, conservation, and accessibility, offering insights into host antiviral ...
Akanksha Choudhary +7 more
wiley +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner +4 more
wiley +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Virus‐like particles (VLPs) are a promising modality with extensive applications in prophylactic and therapeutic vaccine design. Human Papillomavirus (HPV) vaccines are one such example where VLP vaccine applications have led to the successful reduction in HPV‐associated diseases, such as cervical cancer.
Nicholas Samuelson +5 more
wiley +1 more source
, 2018 Additional file 1. Phylogenetic analysis of seven P. falciparum 3D7 DNA endonucleases with homologous proteins of other species. Amino acid sequences were aligned using MEGA 6, and a phylogenetic tree was generated by the neighbour-joining method.
Yiwei Zhang (1448599) +15 more
core +1 more source
BMEMat, EarlyView.TCPL, a transfersome with Tween 80 and PEI, enables efficient transdermal siNF‐κB delivery, resolving PEI's toxicity issue. It promotes lysosomal escape and effective transfection, silences NF‐κB, modulates immunity, and inhibits angiogenesis, alleviating psoriasis. With good biocompatibility, TCPL holds strong clinical translation potential. Schematic
Hui Xing +6 more
wiley +1 more source