Results 131 to 140 of about 55,351 (242)

Editing and synthetic engineering of chloroplast genomes. [PDF]

Plant Commun
Fu J, Zhang J.
europepmc   +1 more source

TKC‐MC: An Effective Strategy for Generating Heritable Heterozygous Mutations in Essential Genes in Rice

Plant Biotechnology Journal, EarlyView.
ABSTRACT The CRISPR/Cas9 gene‐editing technology has been widely used in defining gene functions and crop improvement. However, some genes are essential for plant growth and development. Loss‐of‐function homozygous mutations in essential genes lead to plant death or sterility.
Meilian Xu, Lang Yan, Min Zhu, Zhitian Zhan, Hong Chen, Dantong Wang, Zhenping Zheng, Yujie Zhang, Lizhong Xiong, Yubing He   +9 more
wiley   +1 more source

Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases. [PDF]

Front Plant Sci
Basso MF, Pinto MS, Nonato JVA, Dante RA, Yassitepe JECT.   +4 more
europepmc   +1 more source

Cas9‐Embedding Hyperactive TadA8e Confers Efficient and Highly Specific A‐To‐G Base Editing in Rice

Plant Biotechnology Journal, EarlyView.
ABSTRACT Adenine base editors (ABEs) produce precise A‐to‐G conversion in the genomic target sites without causing double‐strand breaks. However, the hyperactive adenosine deaminase TadA8e raises safety concerns on genome‐wide off‐target edits. We engineered 11 chimeric proteins for ABEs (CP‐ABEs) by embedding hyperactive TadA8e within Cas9 nickase to ...
Jianjian Hu, Xue Li, Yuhong Gao, Yifan Guo, Yini Liu, Chen Wang, Gencheng Xu, Chaoyue Du, Shijia Liu, Zhigang Zhao, Yihua Wang, Yufeng Wu, Xiaoou Dong, Chao Li, Jianmin Wan   +14 more
wiley   +1 more source

Complete Genome Sequences <i>Microbacterium paraoxydans</i> phages BarnCat, Pochacco, Smelly and SpiderBri and <i>Microbacterium foliorum</i> phage Crisis. [PDF]

MicroPubl Biol
Acosta A, Baga M, Ballweber K, Brand K, Cho Y, Cox T, Franco A, Guevara Lopez F, Hernandez F, Hutchins L, Johnson A, Kang H, Kang J, Kim H, Kim M, Klar E, Kwon Y, Lee A, McMillan N, Michel L, Montiel R, Mugenzi S, Murillo L, Nguyen-Khoa E, Nhem T, Peralta N, Role J, Ruiz N, Samayoa A, Sanchez B, Shin A, Terriquez J, Toledo M, Tsao Z, Velasquez A, Dean N, Diaz A.   +36 more
europepmc   +1 more source

Enhancing CRISPR/Cas‐Mediated Gene Knockout With Short Non‐Homologous Oligonucleotides

Plant Biotechnology Journal, EarlyView.
ABSTRACT Chlamydomonas reinhardtii is a model green microalga that has great industrial potential as a sustainable bio‐factory for recombinant protein and high‐value chemical production. Efficient genome editing tools are required to redesign this organism for synthetic biology applications. CRISPR‐Cas editing technologies have already been adapted for
Yen Peng Chew, Aron Ferenczi, Marie Dannay, Cristina Ponce‐Lilly, Adam Kovac, Dávid Tóth, Szilvia Z. Tóth, Attila Molnar   +7 more
wiley   +1 more source

Revealing Cell-Free Mitochondrial DNA Breakage Patterns as Novel Biomarkers for Sepsis. [PDF]

Adv Sci (Weinh)
Liao H, Ma R, Hao S, Tan X, Zeng X, Song R, Chen B, Cao Z, Shen W, Luo Z, Huang J, Huang H, Liu L, Duan C.   +13 more
europepmc   +1 more source

Bibliometric‐Based Analysis of Global Trends and Collaborative Networks in Plant Genetic Engineering (1994–2024)

Plant Biotechnology Journal, EarlyView.
ABSTRACT Agricultural sustainability faces serious challenges from population growth, climate change and ecological degradation. Genetic modification (GM) technology can be regarded as a precise extension of the Green Revolution, aiming to balance yield enhancement with ecological integrity through biotechnology. To systematically examine global trend,
Tongxiao Xu, Teng Wang, Cong Zhang, Yuan Cao, Xiaoyun He   +4 more
wiley   +1 more source

The Biology of Nucleus-Forming Jumbo Phages. [PDF]

Annu Rev Genet
Birkholz EA, Armbruster EG, Pogliano J.
europepmc   +1 more source

Rooting Conifer Genetic Research: An Accessible and Efficient Transformation System

Plant Biotechnology Journal, EarlyView.
ABSTRACT Conifers serve as the cornerstone of global forest ecosystems, yet their genetic transformation faces notorious challenges. To overcome the intrinsic resistance of conifer adventitious roots to Agrobacterium/Rhizobium‐mediated transformation, we systematically and iteratively engineered the binary vector by introducing chimeric Ri plasmid ...
Jing‐Jing Li, Yi‐Mei Zhao, Jia‐Fan Liu, Chen‐He Li, Hui Zhang, Yi‐Tong Song, Quan Zuo, Pei‐Yi Wang, Kai Gao, Dong Meng, Shi‐Hui Niu   +10 more
wiley   +1 more source