Results 191 to 200 of about 155,310 (382)
DNA targeting by compact Cas9d and its resurrected ancestor
Type II CRISPR endonucleases are widely used programmable genome editing tools. Recently, CRISPR-Cas systems with highly compact nucleases have been discovered, including Cas9d (a type II-D nuclease).
Rodrigo Fregoso Ocampo +13 more
doaj +1 more source
Genetic engineering for SIT application: a fruit fly‐focused review
Abstract Sterile insect technique (SIT) has become a key component of efficient pest control. Fruit fly pests from the Drosophilidae and Tephritidae families pose a substantial and overwhelmingly increasing threat to the agricultural industry, aggravated by climate change and globalization among other contributors.
Serafima Davydova +2 more
wiley +1 more source
Iflaviruses in arthropods: when small is mighty
Many arthropod species harbor iflaviruses, which often cause covert (asymptomatic) infections, but may still affect host fitness. We review the impact of iflaviruses on arthropod fitness, immunity, behaviour as well as the iflavirus’ host range, transmission, tissue tropism and the interactions with other microorganisms within arthropods.
Annamaria Mattia +3 more
wiley +1 more source
Mechanism of graphene‐induced cytotoxicity: Role of endonucleases
T. Fahmi +6 more
semanticscholar +1 more source
Bactrocera fruit flies are significant horticultural pests that cause major economic losses. A “neoclassical approach” incorporating genome editing via CRISPR/Cas9 to develop genetic sexing strains (GSS) could render the sterile insect technique (SIT) against these pests more efficient and cost‐effective.
Chrysanthi Ioannidou +5 more
wiley +1 more source
Neoclassical development of genetic sexing strains for insect pest and disease vector control
The sterile insect technique has been effectively used for decades, and an important component is the availability of sex separation systems, in particular genetic sexing strains. Classical approaches, such as irradiation‐induced chromosomal translocations, have yielded stable strains for species like the Mediterranean fruit fly.
Giovanni Petrucci +5 more
wiley +1 more source
Mutational Analysis of Two Putative Catalytic Motifs of the Type IV Restriction Endonuclease Eco57I [PDF]
Renata Rimšelienė, A. Janulaitis
openalex +1 more source
The Ticking Clock: Differential Time‐Dependent Deterioration Between Washed and Thawed Sperm
ABSTRACT Background Cryopreservation is widely used in assisted reproductive technologies. While fresh sperm undergoes gradual time‐dependent deterioration, it remains unclear whether thawed sperm exhibits a more accelerated decline. Objectives To directly compare the rate of deterioration in sperm motility, vitality, and DNA fragmentation between ...
Adiel Kahana +6 more
wiley +1 more source
Endonuclease J: A Site-specific Endonuclease Cleaving Immunoglobulin Genes
S, Kondo +5 more
openaire +2 more sources
Recombinational repair of chromosomal DNA double‐strand breaks generated by a restriction endonuclease [PDF]
Gareth A. Cromie, David R. F. Leach
openalex +1 more source

