Results 61 to 70 of about 106,966 (240)
A novel zinc-finger nuclease platform with a sequence-specific cleavage module [PDF]
, 2011 Zinc-finger nucleases (ZFNs) typically consist of three to four zinc fingers (ZFs) and the non-specific DNA-cleavage domain of the restriction endonuclease FokI.
Cathomen, Toni +5 more
core +2 more sources
Genome editing technologies to fight infectious diseases [PDF]
, 2017 Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa +2 more
core +1 more source
RETRACTED: Bacterial persistence by RNA endonucleases [PDF]
Proceedings of the National Academy of Sciences, 2011 Bacteria form persisters, individual cells that are highly tolerant to different types of antibiotics. Persister cells are genetically identical to nontolerant kin but have entered a dormant state in which they are recalcitrant to the killing activity of the antibiotics. The molecular mechanisms underlying bacterial persistence are unknown.
Maisonneuve E +3 more
openaire +5 more sources
Topological digestion drives time-varying rheology of entangled DNA fluids
Nature Communications, 2022 Understanding and controlling the rheology of polymeric complex fluids is of fundamental importance in both industry and biology. Here, Michieletto et al. show how to achieve time-dependent rheology of DNA solutions via enzymatically-driven architectural
D. Michieletto +6 more
doaj +1 more source
A cleavage map of bacteriophage Phi-X174 genome [PDF]
, 1974 Restriction endonucleases isolated from Hemophilus influenzae, Hemophilus parainfluenzae, and Hemophilus aegyptius were used to cleave phi-X174 replicative form DNA into three sets of specific DNA fragments.
Lee, Amy Shiu, Sinsheimer, Robert L.
core
Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3 [PDF]
, 2010 double Holliday junction (dHJ) is a central intermediate of homologous recombination that can be processed to yield crossover or non-crossover recombination products. To preserve genomic integrity, cells possess mechanisms to avoid crossing over. We show
C Gaillard +37 more
core +2 more sources
A Cas9 with PAM recognition for adenine dinucleotides
Nature Communications, 2020 Protospacer adjacent motif (PAM) requirements limit the target range of CRISPR endonucleases. Here, the authors graft the 5$$^{\prime}$$ ′ -NAAN-3$$^{\prime}$$ ′ PAM-interacting domain of SmacCas9 onto SpyCas9 to create adenine dinucleotide targeting ...
Pranam Chatterjee +7 more
doaj +1 more source
Encyclopedia, 2021 The deoxyribonuclease 1 (Dnase1) family is a key family of endonucleases that degrades DNA. Loss of Dnase1 family function causes several diseases where the host’s immune system targets the host, such as systemic lupus erythematosus, hypocomplementemic ...
Minal Engavale +6 more
doaj +1 more source
Endonucleases and simian virus 40 virions: origin of a virion-associated endonuclease [PDF]
Journal of Virology, 1976 The origin and role of the endonuclease activity associated with purified virions of simian virus 40, previously described by this and other laboratories, have been further investigated. We found that the enzymatic activity from virions of temperature-sensitive (ts) mutants is not more heat labile than that from wild-type virions.
H L Ozer, D C Dooley, M T Ryzlak
openaire +3 more sources
BioTechniques, 1999 REF Select, expert system software, has been developed to assist in the selection of optimal restriction endonucleases for restriction endonuclease fingerprinting (REF), a method for rapid and sensitive mutation screening of long DNA segments (1–2 kb ...
William A. Scaringe +3 more
doaj +1 more source