Results 51 to 60 of about 154,667 (281)

Magnesium rescues the morphology of Bacillus subtilis mreB mutants through its inhibitory effect on peptidoglycan hydrolases

open access: yesScientific Reports, 2022
Cell wall homeostasis in bacteria is tightly regulated by balanced synthesis and degradation of peptidoglycan (PG), allowing cells to expand their sacculus during growth while maintaining physical integrity.
Benoit Tesson   +5 more
doaj   +1 more source

Identifying the histone H3 endopeptidase.

open access: yes, 2014
(a) Schematic of histone H3 endopeptidase enrichment. Whole cell extract from early stationary phase cells was used to purify the histone H3 endopeptidase.
Yong Xue (307664)   +4 more
core   +1 more source

Mesenchymal Stromal Cell‐Mediated Intercellular Communication: Mapping the Interactome for Skeletal Muscle Homeostasis and Regeneration

open access: yesAdvanced Science, EarlyView.
Liu et al. define a systems‐level interactome of fibroadipogenic progenitor (FAP)‐mediated signaling in skeletal muscle by integrating single‐cell transcriptomics with FAP depletion‐based perturbation analysis. Functional interrogation using a conditioned media bioassay links predicted signaling to multicellular outcomes, establishing a framework to ...
Xingyu Liu   +13 more
wiley   +1 more source

Ion exchange chromatography – basic principles and application [PDF]

open access: yes, 2011
Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful ...
Dowling, Oonagh   +3 more
core  

Early Detection and Inhibition of Post‐Surgical Cancer Recurrence by Synthetic Extracellular Vesicles

open access: yesAdvanced Science, EarlyView.
An implantable hydrogel is designed to hold gene transfection agents engineered to turn early recurrent tumor cells into generators of synthetic EVs. These synthetic EVs can express engineered miR‐26a (E‐miR‐26a) for highly sensitive detection and PD‐1 (a PD‐L1‐blocking agent) for therapeutic intervention, thereby enabling early detection and ...
Junli Zhang   +7 more
wiley   +1 more source

PRB1 is required for clipping of the histone H3 N terminal tail in Saccharomyces cerevisiae.

open access: yesPLoS ONE, 2014
Cathepsin L, a lysosomal protein in mouse embryonic stem cells has been shown to clip the histone H3 N- terminus, an activity associated with gene activity during mouse cell development.
Yong Xue   +4 more
doaj   +1 more source

Specificity of prolyl endopeptidase [PDF]

open access: yesFEBS Letters, 1986
A series of tetrapeptides, Cbz(Bz)‐Gly‐X‐Leu‐Gly, were synthesized and the kinetic parameters, k cat and , determined for their hydrolyses by prolyl endopeptidase from Flavobacterium. The peptides with X = N‐Me‐Ala, Sar and Ala as well as the standard substrate (X = Pro) were found to be good substrates, while those with X = α‐aminobutyryl, Hyp, Ser ...
openaire   +2 more sources

Rapid micro methods for the determination of exo- and endopeptidase activities in plant extracts

open access: yes, 1987
Rapid and sensitive photometric assays for aminopeptidase, carboxypeptidase, and endopeptidase activities were developed. The methods of aminopeptidase and carboxypeptidase determinations were based on the hydrolysis of artificial substrates (l-leucine-p-
Salgó, András, Feller, Urs
core   +1 more source

DMS‐MaPseq and DREEM Analyses Implicate the Critical Role of RNA Structural Dynamics in Turnip Yellow Mosaic Virus Pathogenicity

open access: yesAdvanced Science, EarlyView.
RNA structural profiling of Turnip Yellow Mosaic Virus by DMS‐MaPseq and DREEM analyses uncover that viral genome‐wide RSS is highly complicated and heterogeneous, with alternative RSSs widely distributed across the genome. Notably, the viral 3’ tRNA‐like structure adopts alternative conformations in vivo.
Jiaying Zhu   +7 more
wiley   +1 more source

Crystal structure of glycyl endopeptidase from Carica papaya: A cysteine endopeptidase of unusual substrate specificity

open access: yes, 1995
Glycyl endopeptidase is a cysteine endopeptidase of the papain family, characterized by specificity for cleavage C-terminal to glycyl residues only and by resistance to inhibition by members of the cystatin family of cysteine proteinase inhibitors ...
Hemmings, Andrew M.   +3 more
core   +1 more source

Home - About - Disclaimer - Privacy