ESSENTIAL MEIOTIC ENDONUCLEASE 1 is required for chloroplast development and DNA repair in rice
Plant Biotechnology Journal, Volume 23, Issue 7, Page 2931-2948, July 2025.Summary Chloroplast development is fundamental to photosynthesis and plant growth but is sensitive to environmental stress. Chloroplast development and division require genome stability and DNA repair, but the underlying mechanisms have been unclear. Using a forward genetic approach, we identified the striped‐leaf mutant k48 in the rice (Oryza sativa L.
Yanxin Du +5 more
wiley +1 more source
Homodimerisation-independent cleavage of dsRNA by a pestiviral nicking endoribonuclease [PDF]
Scientific Reports, 2018 AbstractThe glycoprotein Erns plays a central role in the biology of the pestivirus bovine viral diarrhea virus (BVDV). This soluble endonuclease mediates the escape from an interferon (IFN) response in the infected fetus, thereby permitting the establishment of persistent infection. Viral single-stranded (ss) and double-stranded (ds) RNA act as potent
Carmela Lussi +2 more
openaire +3 more sources
Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition [PDF]
, 2015 The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements. The fundamental basis of the system is the ability to capture small pieces of foreign DNA for incorporation into the genome at the CRISPR ...
Arslan +34 more
core +4 more sources
Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria
FEBS Open Bio, Volume 15, Issue 6, Page 949-962, June 2025.The transcription and mRNA structures of the alkane biosynthesis genes ado/aar were analysed in the representative cyanobacteria PCC 6803 and SK1‐2‐1, which produce C17H36 and C15H32, respectively. PCC 6803 transconjugants carrying these genes showed a 1.8‐ to 2.3‐fold increase in C17H36 production.
Misato Nagao +4 more
wiley +1 more source
Post-transcriptional control of miRNA biogenesis
RNA: A publication of the RNA Society, 2018 MicroRNAs (miRNAs) are important regulators of gene expression that bind complementary target mRNAs and repress their expression. Precursor miRNA molecules undergo nuclear and cytoplasmic processing events, carried out by the endoribonucleases DROSHA and
G. Michlewski, J. Cáceres
semanticscholar +1 more source
Nucleic Acids Research, 2020 Type III CRISPR–Cas prokaryotic immune systems provide anti-viral and anti-plasmid immunity via a dual mechanism of RNA and DNA destruction. Upon target RNA interaction, Type III crRNP effector complexes become activated to cleave both target RNA (via ...
K. Foster +4 more
semanticscholar +1 more source
Pyrimidine-specific cleavage by an endoribonuclease of Saccharomyces cerevisiae [PDF]
Journal of Bacteriology, 1985 An endoribonuclease with pyrimidine cleavage site specificity was isolated from Saccharomyces cerevisiae. The enzyme had a pH optimum of 6 to 7 and did not require a divalent cation. It was inhibited by 5 X 10(-5) M ethidium bromide, although it appeared to be single strand specific.
openaire +3 more sources
Rex1p Deficiency Leads to Accumulation of Precursor Initiator tRNA\u3csup\u3eMet\u3c/sup\u3e and Polyadenylation of Substrate RNAs in \u3cem\u3eSaccharomyces cerevisiae\u3c/em\u3e [PDF]
, 2009 A synthetic genetic array was used to identify lethal and slow-growth phenotypes produced when a mutation in TRM6, which encodes a tRNA modification enzyme subunit, was combined with the deletion of any non-essential gene in Saccharomyces cerevisiae.
Anderson, James T. +5 more
core +1 more source
RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions [PDF]
, 2011 Prokaryotes have evolved multiple versions of an RNA-guided adaptive immune system that targets foreign nucleic acids. In each case, transcripts derived from clustered regularly interspaced short palindromic repeats (CRISPRs) are thought to selectively ...
Barendregt, Arjan +10 more
core +4 more sources
Opportunities in Therapeutic mRNA Stabilization: Sequence, Structure, Adjuvants and Vectors
Advanced Therapeutics, Volume 8, Issue 6, June 2025.Current mRNA lipid nanoparticles rely on cold storage, which increases the cost and reduces access to the vaccines. As mRNA expands to other clinical opportunities, better methods to stabilize the medicines during shipping, storage, and delivery are needed.
Joshua A. Choe +4 more
wiley +1 more source