Results 61 to 70 of about 233,252 (269)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

The role of semiotic engineering in software engineering [PDF]

Proceedings of the 5th International Workshop on Theory-Oriented Software Engineering, 2016
Semiotic engineering is based upon the semiotic theory of Human-Computer Interaction (HCI), which focuses on communication between designers and users. Semiotic engineering tries to improve users’ interpretation through meta- communication and emphasizes that designers should play the role of legitimate interlocutors in interactive systems.
Vahdat Abdelzad, Timothy C. Lethbridge, Mahmood Hosseini   +2 more
openaire   +1 more source

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

Design method and the development of the low-temperature-difference indirect heating Stirling engine

Nihon Kikai Gakkai ronbunshu, 2018
Low-temperature-difference indirect heating Stirling engine is the most suitable device for a small-scale wood biomass combined heat and power. To design this type of Stirling engine, conventional design method is not sufficient for optimizing working ...
Makoto TAKEUCHI, Shinji SUZUKI, Yutaka ABE   +2 more
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Effect of Diesel Fuel-Coconut Oil-Butanol Blends on Operational Parameters of Diesel Engine

Energies, 2020
The global concentration of greenhouse gasses in the atmosphere is increasing as well as the emissions of harmful pollutants. Utilization of liquid biofuels in combustion engines helps to reduce these negative effects. For diesel engines, the most common
Jakub Čedík, Martin Pexa, Michal Holúbek, Zdeněk Aleš, Radek Pražan, Peter Kuchar   +5 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Flow distribution of supercritical hydrocarbon fuel in parallel regenerative cooling channels under non-uniform heat flux

Propulsion and Energy
Regenerative cooling technology is an important approach for thermal protection in scramjet engines. Non-uniform axial and circumferential heat flow distribution on the combustor leads to improper flow distribution, resulting in reduced cooling ...
Renshuo Zhu, Xinyan Pei, Sihan Zou, Lingyun Hou   +3 more
doaj   +1 more source