Results 111 to 120 of about 308,220 (199)

Large-scale discovery of enhancers from human heart tissue. [PDF]

, 2011
Development and function of the human heart depend on the dynamic control of tissue-specific gene expression by distant-acting transcriptional enhancers.
Afzal, Veena, Akiyama, Jennifer A, Black, Brian L, Blow, Matthew J, Bristow, James, Holt, Amy, Jensen, Brian C, Kaplan, Tommy, May, Dalit, McCulley, David J, Pennacchio, Len A, Plajzer-Frick, Ingrid, Rubin, Edward M, Shoukry, Malak, Simpson, Paul C, Visel, Axel, Wright, Crystal   +16 more
core  

Metal-Enhanced Fluorescence-Based RNA Sensing

Journal of the American Chemical Society, 2006
In this communication, we present the proof-of-principle of a new RNA sensing technique, based on metal-enhanced fluorescence, which may improve the potential of solid substrate-based RNA capture assays. In this regard, the detection of RNA is accomplished by annealing a target RNA tagged with a fluorophore to a DNA anchor probe in a single step on a ...
Kadir, Aslan, Jun, Huang, Gerald M, Wilson, Chris D, Geddes   +3 more
openaire   +2 more sources

RACK7 senses and fine-tunes enhancer activity

iScience
Summary: Enhancers regulate dynamic gene expression in a spatiotemporal specific manner. A number of positive as well as negative regulators of enhancers have been reported so far.
Li Dong, Daixuan Zhang, Ying Cai, Min Zeng, Mandi Mu, Peng Zhao, Jin Wang, Siyi Cheng, Qingqing Cai, Jian Zhang, Yajun Hu, Lei Zhang, Li Tan, Feizhen Wu, Yujiang Geno Shi, Wenqi Xu, Hongjie Shen   +16 more
doaj   +1 more source

motifDiverge: a model for assessing the statistical significance of gene regulatory motif divergence between two DNA sequences

, 2014
Next-generation sequencing technology enables the identification of thousands of gene regulatory sequences in many cell types and organisms. We consider the problem of testing if two such sequences differ in their number of binding site motifs for a ...
Friedrich, Tara, Holloway, Alisha K., Kostka, Dennis, Pollard, Katherine S.   +3 more
core   +1 more source

Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements. [PDF]

, 2019
Pooled CRISPR-Cas9 screens are a powerful method for functionally characterizing regulatory elements in the non-coding genome, but off-target effects in these experiments have not been systematically evaluated.
Aradhana, Bassik, Michael C, Bintu, Lacramioara, Ego, Braeden K, Greenleaf, William J, Greenside, Peyton G, Hess, Gaelen T, Kaplow, Irene M, Kundaje, Anshul, Li, Amy, Marinov, Georgi K, Morgens, David W, Phanstiel, Douglas H, Snyder, Michael P, Spees, Kaitlyn, Trevino, Alexandro E, Truong, Alisa, Tycko, Josh, Ursu, Oana, Wainberg, Michael, Yao, David   +20 more
core  

Comprehensive Pan-Cancer Mutation Density Patterns in Enhancer RNA. [PDF]

Int J Mol Sci, 2023
Zhang T, Yu H, Jiang L, Bai Y, Liu X, Guo Y.   +5 more
europepmc   +1 more source

Enhancing RNA editing efficiency and specificity with engineered ADAR2 guide RNAs

Molecular Therapy Nucleic Acids
RNA editing is a prospective therapeutic approach for correcting harmful mutations, offering the benefits of reversibility and tunability without permanently modifying the genome. However, the relatively low enzymatic activity and the occurrence of off-target editing events present significant challenges, limiting its utility.
Xilei Ai, Sheng Ding, Shan Zhou, Feng Du, Shuai Liu, Xin Cui, Juan Dong, Xin Huang, Zhuo Tang   +8 more
openaire   +2 more sources

DNA G-quadruplex structures act as functional elements in α- and β-globin enhancers

Genome Biology
Background Enhancer elements interact with target genes at a distance to modulate their expression, but the molecular details of enhancer–promoter interaction are incompletely understood.
Colm Doyle, Krzysztof Herka, Sean M. Flynn, Larry Melidis, Somdutta Dhir, Stefan Schoenfelder, David Tannahill, Shankar Balasubramanian   +7 more
doaj   +1 more source

Cell-type-specific loops linked to RNA polymerase II elongation in human neural differentiation

Cell Genomics
Summary: DNA is folded into higher-order structures that shape and are shaped by genome function. The role of long-range loops in the establishment of new gene expression patterns during cell fate transitions remains poorly understood.
Katelyn R. Titus, Zoltan Simandi, Harshini Chandrashekar, Dominik Paquet, Jennifer E. Phillips-Cremins   +4 more
doaj   +1 more source

Crystal structure of a cap-independent translation enhancer RNA. [PDF]

Nucleic Acids Res, 2023
Lewicka A, Roman C, Jones S, Disare M, Rice PA, Piccirilli JA.   +5 more
europepmc   +1 more source