Results 91 to 100 of about 308,220 (199)
EBioMedicine, 2019 Background: The injured liver loses normal function, with concomitant decrease of key identity genes. Super-enhancers contribute to mammalian cell identity. Here, we identified core transcription factors (TFs) that are active in hepatocytes, using genome-
Min Sung Joo +4 more
doaj +1 more source
A Region in the 3′ UTR of MnSOD RNA Enhances Translation of a Heterologous RNA
Biochemical and Biophysical Research Communications, 2000 The studies reported in this paper were designed to test the hypothesis that a cis element located in the 3' UTR of manganese superoxide dismutase (MnSOD) RNA, designated MnSOD-response element (MnSOD-RE), is a translational enhancer in vivo. NIH/3T3 cells were transfected with a posttranscriptional reporter construct in which MnSOD-RE was placed 3' of
L, Knirsch, L B, Clerch
openaire +2 more sources
The RNA Polymerase II Core Promoter in Drosophila. [PDF]
, 2019 Transcription by RNA polymerase II initiates at the core promoter, which is sometimes referred to as the "gateway to transcription." Here, we describe the properties of the RNA polymerase II core promoter in Drosophila The core promoter is at a strategic
Kadonaga, James T +2 more
core +1 more source
Creating New β-Globin-Expressing Lentiviral Vectors by High-Resolution Mapping of Locus Control Region Enhancer Sequences. [PDF]
, 2020 Hematopoietic stem cell gene therapy is a promising approach for treating disorders of the hematopoietic system. Identifying combinations of cis-regulatory elements that do not impede packaging or transduction efficiency when included in lentiviral ...
Aleshe, Bamidele +14 more
core +1 more source
Solid phase chemistry to covalently and reversibly capture thiolated RNA. [PDF]
, 2018 Here, we describe an approach to enrich newly transcribed RNAs from primary mouse neurons using 4-thiouridine (s4U) metabolic labeling and solid phase chemistry.
Canzio, Daniele +3 more
core +1 more source
Genome BiologyBackground RNA-RNA spatial interactions play crucial roles in various cellular processes, including gene transcriptional and post-transcriptional regulations. However, research on this area of RNA regulation remains limited in plants.
Miaomiao Wen +6 more
doaj +1 more source
Gene isoform specificity through enhancer-associated antisense transcription.
PLoS ONE, 2012 Enhancers and antisense RNAs play key roles in transcriptional regulation through differing mechanisms. Recent studies have demonstrated that enhancers are often associated with non-coding RNAs (ncRNAs), yet the functional role of these enhancer:ncRNA ...
Courtney S Onodera +6 more
doaj +1 more source
Shep RNA-Binding Capacity Is Required for Antagonism of gypsy Chromatin Insulator Activity
G3: Genes, Genomes, Genetics, 2019 Chromatin insulators are DNA-protein complexes that regulate chromatin structure and gene expression in a wide range of organisms. These complexes also harbor enhancer blocking and barrier activities.
Dahong Chen +3 more
doaj +1 more source
Rescue of splicing-mediated intron loss maximizes expression in lentiviral vectors containing the human ubiquitin C promoter. [PDF]
, 2014 Lentiviral vectors almost universally use heterologous internal promoters to express transgenes. One of the most commonly used promoter fragments is a 1.2-kb sequence from the human ubiquitin C (UBC) gene, encompassing the promoter, some enhancers, first
Cooper, Aaron R +3 more
core +1 more source