Results 91 to 100 of about 2,925 (189)

Alistipes senegalensis is Critically Involved in Gut Barrier Repair Mediated by Panax Ginseng Neutral Polysaccharides in Aged Mice

Advanced Science, Volume 12, Issue 36, September 25, 2025.
An integral functional axis through which GPN and functional metabolites of Alistipes senegalensis can hydrolyze tryptophan into indoles. Furthermore, the Alistipes senegalensis‐indole‐AhR pathway is found to be associated with increased expression of tight junction‐associated proteins and activation of gut stem cells.
Dandan Wang, Hui Wang, Yingna Li, Jing Lu, Xiaolei Tang, Dan Yang, Manying Wang, Daqing Zhao, Fangbing Liu, Shuai Zhang, Liwei Sun   +10 more
wiley   +1 more source

Ex Vivo Enteroids Recapitulate In Vivo Citrulline Production in Mice [PDF]

The Journal of Nutrition, 2018
The endogenous production of arginine relies on the synthesis of citrulline by enteral ornithine transcarbamylase (OTC). Mutations in the gene coding for this enzyme are the most frequent cause of urea cycle disorders. There is a lack of correlation between in vivo metabolic function and DNA sequence, transcript abundance, or in vitro enzyme activity ...
Xiaoying Wang, Yang Yuan, Inka C Didelija, Mahmoud A Mohammad, Juan C Marini   +4 more
openaire   +2 more sources

Opinion: Are Organoids the End of Model Evolution for Studying Host Intestinal Epithelium/Microbe Interactions?

Microorganisms, 2019
In the pursuit to understand intestinal epithelial cell biology in health and disease, researchers have established various model systems, from whole animals (typically rodents) with experimentally induced disease to transformed human carcinomas.
Michelle M. George, Mushfiqur Rahman, Jessica Connors, Andrew W. Stadnyk   +3 more
doaj   +1 more source

Protocol for the cryopreservation of Cryptosporidium isolates using enteroids. [PDF]

STAR Protoc
A major bottleneck in the progress of Cryptosporidium research is the lack of accessible cryopreservation of Cryptosporidium oocysts. Here, we present a protocol for the cryopreservation of Cryptosporidium isolates using enteroids. We describe the steps for the establishment of enteroid cultures and cryopreservation of C. parvum-infected HCT-8 cultures.
Deng M, Wei Y, Li N, Xiao L, Feng Y, Guo Y.   +5 more
europepmc   +4 more sources

The Role of Iron Chelation Therapy in Colorectal Cancer: A Systematic Review on Its Mechanisms and Therapeutic Potential

Cancer Medicine, Volume 14, Issue 13, July 2025.
Iron chelation is a promising yet underexplored strategy for colorectal cancer (CRC) treatment. Iron chelators have a dual role in inhibiting and potentially promoting cancer progression. Iron chelation affects key CRC pathways, including histone methylation, NDRG1 upregulation, Wnt/β‐catenin, and p53 modulation.
Gihani Vidanapathirana, Md Sajedul Islam, Sujani Gamage, Alfred K. Lam, Vinod Gopalan   +4 more
wiley   +1 more source

Engineered Tissue Models to Decode Host–Microbiota Interactions

Advanced Science, Volume 12, Issue 23, June 20, 2025.
Host–Microbiota interactions in the human body. Created in BioRender. Ghezzi, C. (2025) https://BioRender.com/ihivskg. Abstract A mutualistic co‐evolution exists between the host and its associated microbiota in the human body. Bacteria establish ecological niches in various tissues of the body, locally influencing their physiology and functions, but ...
Miryam Adelfio, Grace E. Callen, Xuesong He, Bruce J. Paster, Hatice Hasturk, Chiara E. Ghezzi   +5 more
wiley   +1 more source

A Dynamic Double‐Flow Gut‐On‐Chip Model for Predictive Absorption Studies In Vitro

Advanced Materials Technologies, Volume 10, Issue 9, May 6, 2025.
This study presents a novel millifluidic gut‐on‐chip model using the MIVO® device to replicate human intestinal conditions. Dynamic co‐culture of CaCo‐2 and HT‐29 cells accelerates differentiation, modulates mucus thickness, and enhances barrier function.
Maria Elisabetta Federica Palamà, Maurizio Aiello, Gergő Borka, Jacopo Furci, Ilaria Parodi, Giuseppe Firpo, Silvia Scaglione   +6 more
wiley   +1 more source

Identification of Reg3β-producing cells using IL-22-stimulated enteroids

Bioscience, Biotechnology, and Biochemistry, 2020
ABSTRACT Reg3β, a lectin, displays antibacterial activity. This study investigated Reg3β-expressing cells using IL-22-stimulated enteroids. IL-22 stimulation elevated the mRNA and protein levels of Reg3β. IL-22 also increased the mRNA levels of CD133 (a transit-amplifying cell marker) and lysozyme (a Paneth cell marker ...
Mika, Sato, Akihiko, Inaba, Ken, Iwatsuki, Yuki, Saito, Miki, Tadaishi, Makoto, Shimizu, Kazuo, Kobayashi-Hattori   +6 more
openaire   +2 more sources

Replication of human noroviruses in stem cell–derived human enteroids [PDF]

Science, 2016
The major barrier to research and development of effective interventions for human noroviruses (HuNoVs) has been the lack of a robust and reproducible in vitro cultivation system. HuNoVs are the leading cause of gastroenteritis worldwide. We report the successful cultivation of multiple HuNoV strains in enterocytes in stem cell–derived, nontransformed ...
Khalil, Ettayebi, Sue E, Crawford, Kosuke, Murakami, James R, Broughman, Umesh, Karandikar, Victoria R, Tenge, Frederick H, Neill, Sarah E, Blutt, Xi-Lei, Zeng, Lin, Qu, Baijun, Kou, Antone R, Opekun, Douglas, Burrin, David Y, Graham, Sasirekha, Ramani, Robert L, Atmar, Mary K, Estes   +16 more
openaire   +2 more sources

Hemagglutinin Protease HapA Associated With Vibrio cholerae Outer Membrane Vesicles (OMVs) Disrupts Tight and Adherens Junctions

Journal of Extracellular Vesicles, Volume 14, Issue 5, May 2025.
ABSTRACT This study explores the virulence mechanisms of Vibrio cholerae, with a particular emphasis on HapA, a zinc metalloprotease delivered via outer membrane vesicles (OMVs). The findings reveal that OMV‐associated HapA disrupts the integrity of tight and adherens junctions in intestinal epithelial cell models more effectively than its purified ...
Palwasha Baryalai, David Irenaeus, Eric Toh, Madeleine Ramstedt, Bernt Eric Uhlin, Aftab Nadeem, Sun Nyunt Wai   +6 more
wiley   +1 more source