Results 111 to 120 of about 2,925 (189)

Use of Human Intestinal Enteroids to Detect 
Human Norovirus Infectivity

Emerging Infectious Diseases, 2019
Tools to detect human norovirus infectivity have been lacking. Using human intestinal enteroid cultures inoculated with GII.Pe-GII.4 Sydney–infected fecal samples, we determined that a real-time reverse transcription PCR cycle threshold cutoff of 30 may ...
Martin Chi-Wai Chan, Sarah K.C. Cheung, Kirran N. Mohammad, Jenny C.M. Chan, Mary K. Estes, Paul K.S. Chan   +5 more
doaj   +1 more source

Long-term culture captures injury-repair cycles of colonic stem cells [PDF]

, 2019
The colonic epithelium can undergo multiple rounds of damage and repair, often in response to excessive inflammation. The responsive stem cell that mediates this process is unclear, in part because of a lack of in vitro models that recapitulate key ...
Chen, Feidi, Cheng, Jiye, Chiang, I-Ling, Fujii, Satoru, Gordon, Jeffrey I, Han, Nathan D, Janova, Hana, Lai, Chin-Wen, Liu, Ta-Chiang, Lu, Qiuhe, Muegge, Brian D, Ohara, Takahiro E, Sibley, L David, Stappenbeck, Thaddeus S, VanDussen, Kelli L, Ver Heul, Aaron, Wang, Yi, Whitehurst, Charles E, Wu, Renee, Xiong, Shanshan   +19 more
core   +1 more source

Using primary murine intestinal enteroids to study dietary TAG absorption, lipoprotein synthesis, and the role of apoC-III in the intestine[S]

Journal of Lipid Research, 2017
Since its initial report in 2009, the intestinal enteroid culture system has been a powerful tool used to study stem cell biology and development in the gastrointestinal tract. However, a major question is whether enteroids retain intestinal function and
Javeed Jattan, Cayla Rodia, Diana Li, Adama Diakhate, Hongli Dong, Amy Bataille, Noah F. Shroyer, Alison B. Kohan   +7 more
doaj   +1 more source

Human enteroid monolayers as a potential alternative for Ussing chamber and Caco-2 monolayers to study passive permeability and drug efflux [PDF]

After oral administration, the intestine is the first site of drug absorption, making it a key determinant of the bioavailability of a drug, and hence drug efficacy and safety. Existing non-clinical models of the intestinal barrier in vitro often fail to
Bervoets, Sander, de Wildt, Saskia N., Greupink, Rick, Keuper-Navis, Marit, O'Gorman, Luke, Russel, Frans G.M., Stommel, Martijn W.J., Streekstra, Eva J., van de Steeg, Evita, van den Broek, Petra, van den Heuvel, Jeroen J.M.W.   +10 more
core   +2 more sources

Equine coronavirus infection and replication in equine intestinal enteroids

Veterinary Research
Abstract In this study, equine intestinal enteroids (EIEs) were generated from the duodenum, jejunum, and ileum and inoculated with equine coronavirus (ECoV) to investigate their suitability as in vitro models with which to study ECoV infection. Immunohistochemistry revealed that the EIEs were composed of various cell types expressed in vivo in
Yoshinori Kambayashi, Manabu Nemoto, Akihiro Ochi, Daiki Kishi, Takanori Ueno, Koji Tsujimura, Hiroshi Bannai, Nanako Kawanishi, Minoru Ohta, Tohru Suzuki   +9 more
openaire   +3 more sources

The potential of enteroids derived from children and adults to study age-dependent differences in intestinal CYP3A4/5 metabolism [PDF]

Drug metabolism in the intestinal wall affects bioavailability of orally administered drugs and is influenced by age. Hence, it is important to fully understand the drug metabolizing capacity of the gut to predict systemic exposure. The aim of this study
Botden, Sanne M.B.I., de Boode, Willem P., de Wildt, Saskia N., Greupink, Rick, Keuper-Navis, Marit, Russel, Frans G.M., Stommel, Martijn W.J., Streekstra, Eva J., van de Steeg, Evita, van den Broek, Petra, van den Heuvel, Jeroen J.W.M.   +10 more
core   +2 more sources

In Vitro Culture of Human Norovirus in the Last 20 Years

Biomedicines
Human noroviruses (HuNoVs) are the main pathogens that cause acute gastroenteritis and lead to huge economic losses annually. Due to the lack of suitable culture systems, the pathogenesis of HuNoVs and the development of vaccines and drugs have ...
Chao Cheng, Xia Cai, Jingjing Li, Xiaomeng Zhang, Youhua Xie, Junqi Zhang   +5 more
doaj   +1 more source

Working with patient-derived enteroids and colonoids v1

, 2023
This is a compilations of working with patient-derived enteroids or colonoids, from recovery to cryopreservation, in expansion culture (minimal differentiation)
openaire   +1 more source

Working with patient-derived enteroids and colonoids v2

, 2023
This is a compilations of protocols for working with patient-derived enteroids or colonoids, from recovery to cryopreservation, in expansion culture (minimal differentiation)
openaire   +1 more source

Cultivation, cryopreservation, and transcriptomic studies of host-adapted Cryptosporidium parvum and Cryptosporidium hominis using enteroids

iScience
Summary: Cryptosporidium hominis and Cryptosporidium parvum are major causes of severe diarrhea. Comparative studies of them are hampered by the lack of effective cultivation and cryopreservation methods, especially for C. hominis.
Miner Deng, Tianyi Hou, Jie Zhang, Xinjie Mao, Fuxian Yang, Yanting Wei, Yongping Tang, Wanting Zeng, Wanyi Huang, Na Li, Lihua Xiao, Yaoyu Feng, Yaqiong Guo   +12 more
doaj   +1 more source