Results 111 to 120 of about 175,424 (290)

Using phylogeny and functional traits for assessing community assembly of alpine shrubs along environmental gradients in Three-River-Source Nature Reserve, China

Frontiers in Ecology and Evolution
IntroductionThe increasing impacts of climate change and anthropogenic activities on ecological communities pose substantial challenges to alpine ecosystems.
Min Li, Min Li, Wenxu Cao, Wenxu Cao, Xu Li, Xu Li, Qinghe Li, Qinghe Li   +7 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Thermal Niche Differentiation Shapes the Hibernating Bat Assemblages in Bulgarian Caves Across an Elevational Gradient

Biology
Elevation is a strong proxy for the thermal environment because it causes a predictable drop in temperature and food availability. This restricts cave-dwelling bats to species with specific metabolic traits, such as torpor or migration to avoid cold ...
Heliana Dundarova, Ilya Acosta-Pankov, Elena Nedyalkova, Andrea Lubenova, Maksim Kolev, Krasimir Kirov, Krasimir Lakovski, Olya Genova, Valeri Parvanov, Plamenka Iskrenova, Vladimir Trifonov, Tsenka Chassovnikarova   +11 more
doaj   +1 more source

High-precision temporal interferometry from nonlinearly-spaced phase-shifted interferograms through spatial-filtering

, 2019
We present a high-precision temporal-spatial phase-demodulation algorithm for phase-shifting interferometry (PSI) affected by random/systematic phase-stepping errors.
Guillermo Garnica (16032587), Gonzalo Paez (14511005), Manuel Servin (14511002), Moises Padilla (14510999)   +3 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Multiscale patterns of sponge diversity on Western Atlantic coral reefs: insights from the Southern Gulf of Mexico and the Mexican Caribbean

Frontiers in Marine Science
IntroductionSponges are increasingly dominant components of coral reefs in the Tropical Western Atlantic, yet patterns of their diversity across spatial scales remain poorly quantified.MethodsWe applied a hierarchical sampling design across 13 reefs and ...
Diana Ugalde, Nuno Simões, Nuno Simões, Nuno Simões, María Cristina Díaz, María Cristina Díaz, Edlin Guerra-Castro, Edlin Guerra-Castro   +7 more
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source