Results 101 to 110 of about 175,424 (290)
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Environmental governance in Southern Europe: the domestic filters of Europeanisation
Environmental Politics, 2010 Over the last two decades, the EU has shown a preference for the replacement of traditional command-and-control patterns of environmental regulation with more cooperative policy schemes in the expectation of improving compliance in member states. But has the EU performed as a ‘governance-shaper’ in member states, such as Spain, Portugal and Greece that
Fernández, A.M. +2 more
openaire +2 more sources
Environmental Drivers Override Host Phylogeny in a Locoweed–Endophyte Symbiosis
Journal of FungiPlant endophytes, often termed the “second genome”, critically shape host adaptability. However, the complexity of their interactions, regulated by microbial traits, host species, and environment, has limited both our understanding of symbiosis and the ...
Yue-Yang Zhang +2 more
doaj +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
, 2020 Species distribution models (SDMs) are widely used in ecology and conservation. However, their performance is known to be affected by a variety of factors related to species occurrence characteristics. In this study, we used a virtual species approach to
Vítězslav Moudrý +7 more
core +1 more source
A Novel Filtering Method of 3D Reconstruction Point Cloud from Tomographic SAR
, 2023 With the development of airborne synthetic aperture radar (SAR) technology, the 3D SAR point cloud reconstruction has emerged as a crucial development trend in the current SAR community.
Liangjiang Zhou +4 more
core +1 more source
FEBS Open Bio, EarlyView.In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes +32 more
wiley +1 more source
AnimalsScale mites of the genus Geckobia (Pterygosomatidae) are highly specialized permanent parasites of geckos, but their diversity and ecology in arid environments remain poorly understood.
Monika Fajfer-Jakubek, Bożena Sikora
doaj +1 more source
Metacommunity Structure of Stream Insects across Three Hierarchical Spatial scales
Ecology and Evolution, 2020 A major challenge in community ecology is to understand the underlying factors driving metacommunity (i.e., a set of local communities connected through species dispersal) dynamics.
Siwen He +3 more
doaj +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source