Results 61 to 70 of about 638,267 (297)

Soft motions encoded by the fold and mediated by conserved hinge sites drive V‐ATPase allosteric machinery and autoinhibition

FEBS Letters, EarlyView.
Vacuolar H+‐ATPase (V‐ATPase) is a multisubunit rotary proton pump that acidifies intracellular compartments. Using elastic network models, we identified conserved hinge residues that govern its large‐scale functional motions. We show that intrinsically encoded ‘soft modes’ drive critical functional transitions, including rotary catalysis and ...
Diksha Parwana, Xiaowei Bogetti, Stuti Sharma, Ivet Bahar   +3 more
wiley   +1 more source

Enzymatic Hydrolysis of Pteroylpolyglutamates in Cabbage

Experimental Biology and Medicine, 1972
SummaryThe presence of folate conjugase(s) was established in cabbage. The conjugase(s) was found to have its pH optimum at 5.0 and 8.0 in acetate buffer and Tris buffer, respectively. At pH 8.0 this conjugase was able to split down to third gamma-linkage yielding pteroyltriglutamate while the polyglutamates were split to monoglutamate at pH 5.0.
T, Tamura, K U, Buehring, E L, Stokstad
openaire   +2 more sources

Additives enhancing enzymatic hydrolysis of lignocellulosic biomass

, 2017
Linked to the development of cellulolytic enzyme cocktails from Myceliophthora thermophila, we studied the effect of different additives on the enzymatic hydrolysis yield.
Rocha Martín, Javier, Javier Rocha-Martín, Reyes-Sosa, Francisco Manuel, Claudio Martinez-Bernal, Pérez-Cobas, Yolanda, Bruno Díez García, Martínez-Bernal, Claudio, Díez García, Bruno, Yolanda Pérez-Cobas, Francisco Manuel Reyes-Sosa   +9 more
core   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of Saccharina japonica Biomass

Energies, 2021
This study investigated the production of fermentable sugars from carbohydrate-rich macroalgae Saccharina japonica using sequential hydrolysis (hydrothermal acid pretreatment and enzymatic hydrolysis) to determine the maximum reducing sugar yield (RSy ...
Eun-Young Park, Jung-Kyu Park
doaj   +1 more source

Enzyme-induced Formation of ß-Lactoglobulin Fibrils by AspN Endoproteinase [PDF]

, 2008
This paper describes a low temperature, enzymatic route to induce fibrillar structures in a protein solution. The route comprises two steps. First, ß-lactoglobulin was hydrolyzed into peptides at pH 8 and 37°C with the enzyme AspN endoproteinase, which ...
Venema, P., Goot, A.J., van der, Linden, E., van der, Akkermans, C., Boom, R.M., Erik van der Linden, Atze Jan van der Goot, Remko M. Boom, Cynthia Akkermans, Paul Venema   +9 more
core   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Optimization of the dilute maleic acid pretreatment of wheat straw [PDF]

, 2009
Background In this study, the dilute maleic acid pretreatment of wheat straw is optimized, using pretreatment time, temperature and maleic acid concentration as design variables. A central composite design was applied to the experimental set up.
Hendrik H Beeftink, Elinor L Scott, Johan PM Sanders, Scott, E.L., Sanders, J.P.M., Kootstra, A.M.J., Sanders Johan PM, Beeftink, H.H., A Maarten J Kootstra, Scott Elinor L, Kootstra A Maarten J, Beeftink Hendrik H   +11 more
core   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source