Results 31 to 40 of about 671,790 (405)

Dynamics of Mycobacterium tuberculosis Ag85B Revealed by a Sensitive Enzyme-Linked Immunosorbent Assay. [PDF]

open access: yes, 2019
Secretion of specific proteins contributes to pathogenesis and immune responses in tuberculosis and other bacterial infections, yet the kinetics of protein secretion and fate of secreted proteins in vivo are poorly understood. We generated new monoclonal
Bolz, Miriam   +2 more
core   +2 more sources

Effect of Spacer and the Enzyme-Linked Immunosorbent Assay [PDF]

open access: yes, 2016
The effect of spacers and the enzyme-linked immunosorbent assay (ELISA) formats on the functional parameters of assays such as lower detection limit, inhibitory concentration at 50 per cent (IC50), and specificity were studied.
Agrawal, Sumit   +3 more
core   +2 more sources

Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites

open access: yesJournal of Natural Medicines, 2017
Immunoassays are antibody-based analytical methods for quantitative/qualitative analysis. Since the principle of immunoassays is based on specific antigen–antibody reaction, the assays have been utilized worldwide for diagnosis, pharmacokinetic studies ...
S. Sakamoto   +6 more
semanticscholar   +1 more source

Monitoring Disease Activity in Systemic Lupus Erythematosus With Single‐Molecule Array Digital Enzyme‐Linked Immunosorbent Assay Quantification of Serum Interferon‐α

open access: yesArthritis & Rheumatology, 2019
No simple or standardized assay is available to quantify interferon‐α (IFNα) in routine clinical practice. Single‐molecule array (Simoa) digital enzyme‐linked immunosorbent assay (ELISA) technology enables direct IFNα quantification at attomolar ...
A. Mathian   +18 more
semanticscholar   +1 more source

Development of a Simple Pretreatment Immunoassay Based on an Organic Solvent-Tolerant Nanobody for the Detection of Carbofuran in Vegetable and Fruit Samples

open access: yesBiomolecules, 2019
Nanobodies are one-tenth the size of conventional antibodies and are naturally obtained from the atypical heavy-chain-only antibodies present in camelids.
Jin-ru Zhang   +10 more
doaj   +1 more source

One-step, Wash-free, Nanoparticle Clustering-based Magnetic Particle Spectroscopy (MPS) Bioassay Method for Detection of SARS-CoV-2 Spike and Nucleocapsid Proteins in Liquid Phase [PDF]

open access: yesarXiv, 2021
With the ongoing global pandemic of coronavirus disease 2019 (COVID-19), there is an increasing quest for more accessible, easy-to-use, rapid, inexpensive, and high accuracy diagnostic tools. Traditional disease diagnostic methods such as qRT-PCR (quantitative reverse transcription-PCR) and ELISA (enzyme-linked immunosorbent assay) require multiple ...
arxiv  

Allergen-Based Diagnostic: Novel and Old Methodologies with New Approaches [PDF]

open access: yes, 2017
This chapter is an extensive review of allergen-based diagnostic methodologies including old techniques such as skin prick test, radio-allergo sorbent test, enzyme-linked immunosorbent assay, and fluorescent-enzyme immunosorbent assay. Novel technologies
Ayala-Balboa, Julio   +3 more
core   +1 more source

The ELISA, Enzyme-Linked Immunosorbent Assay [PDF]

open access: yesClinical Chemistry, 2010
Featured Article: Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971;8:871–4.1 This paper was my first as a graduate student of Professor Peter Perlmann at Stockholm University.
openaire   +2 more sources

Using Enzyme-Linked Aptamer Assay to Detect Hepatitis B Surface Antigen in Comparison to Enzyme-Linked Immunosorbent Assay

open access: yesمجله دانشکده پزشکی اصفهان, 2018
Background: Clinical diagnostic kits for detecting hepatitis B are based on antibodies, and have inefficient sensitivity, stability, and cost. Therefore, researches about aptamers and using them instead of antibodies may make these defects up.
Hamidreza Mianesaz   +4 more
doaj   +1 more source

A new enzyme-linked immunosorbent assay for neurofilament light in cerebrospinal fluid: analytical validation and clinical evaluation

open access: yesAlzheimer's Research & Therapy, 2018
Cerebrospinal fluid (CSF) neurofilament light (NfL) is a reliable marker of neuro-axonal damage in different neurological disorders that is related to disease severity.
L. Gaetani   +10 more
semanticscholar   +1 more source

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