Results 41 to 50 of about 6,038,018 (411)

Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker.

open access: yesJournal of Investigative Dermatology, 1982
We examined the hypothesis that myeloperoxidase (MPO), a plentiful constituent of neutrophils, might serve as a marker for tissue neutrophil content. To completely extract MPO from either neutrophils or skin, hexadecyltrimethylammonium bromide (HTAB) was
P. Bradley   +3 more
semanticscholar   +1 more source

Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 1990
Nitric oxide mediates vascular relaxing effects of endothelial cells, cytotoxic actions of macrophages and neutrophils, and influences of excitatory amino acids on cerebellar cyclic GMP.
D. Bredt, S. Snyder
semanticscholar   +1 more source

Enzymes as chaperones and chaperones as enzymes

open access: yesFEBS Letters, 1998
Chaperones and foldases are two groups of accessory proteins which assist maturation of nascent peptides into functional proteins in cells. Protein disulfide isomerase, a foldase, and ATP‐dependent proteases, responsible for degradation of misfolded proteins in cells, both have intrinsic chaperone activities.
Chen Lu Tsou, Chih-chen Wang
openaire   +3 more sources

Kinetic Characterization and X-ray Structure of a Mutant of Haloalkane Dehalogenase with Higher Catalytic Activity and Modified Substrate Range [PDF]

open access: yes, 1996
Conversion of halogenated aliphatics by haloalkane dehalogenase proceeds via the formation of a covalent alkyl-enzyme intermediate which is subsequently hydrolyzed by water.
Dijkstra, Bauke W.,   +7 more
core   +3 more sources

The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis

open access: yesMicrobial Cell Factories, 2020
Background Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed.
Kristina Volkenborn   +5 more
doaj   +1 more source

Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle

open access: yesMicrobiome, 2021
Background RNA interference (RNAi) has emerged as an efficient tool to control insect pests. When insects ingest double-stranded RNAs (dsRNAs) targeted against essential genes, strong gene silencing and mortality can be induced.
Letian Xu   +6 more
doaj   +1 more source

Genome-wide study of Cerrena unicolor 87613 laccase gene family and their mode prediction in association with substrate oxidation

open access: yesBMC Genomics, 2023
Background Laccases are green biocatalysts with wide industrial applications. The study of efficient and specific laccase producers remains a priority.
Long-Bin Zhang   +2 more
doaj   +1 more source

Clinical Utility of SARS-CoV-2 Antibody Titer Multiplied by Binding Avidity of Receptor-Binding Domain (RBD) in Monitoring Protective Immunity and Clinical Severity

open access: yesViruses, 2023
Conventional serum antibody titer, which expresses antibody level, does not provide antigen binding avidity of the variable region of the antibody, which is essential for the defense response to infection.
Etsuhisa Takahashi   +11 more
doaj   +1 more source

A new intrinsic thermal parameter for enzymes reveals true temperature optima [PDF]

open access: yes, 2004
Two established thermal properties of enzymes are the Arrhenius activation energy and thermal stability. Arising from anomalies found in the variation of enzyme activity with temperature, a comparison has been made of experimental data for the activity ...
Daniel, Roy M.   +4 more
core   +2 more sources

The effect of angiotensin-converting-enzyme inhibition on diabetic nephropathy. The Collaborative Study Group.

open access: yesNew England Journal of Medicine, 1993
BACKGROUND Renal function declines progressively in patients who have diabetic nephropathy, and the decline may be slowed by antihypertensive drugs. The purpose of this study was to determine whether captopril has kidney-protecting properties independent
E. Lewis   +3 more
semanticscholar   +1 more source

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