Results 41 to 50 of about 6,038,018 (411)
Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker.
We examined the hypothesis that myeloperoxidase (MPO), a plentiful constituent of neutrophils, might serve as a marker for tissue neutrophil content. To completely extract MPO from either neutrophils or skin, hexadecyltrimethylammonium bromide (HTAB) was
P. Bradley+3 more
semanticscholar +1 more source
Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme.
Nitric oxide mediates vascular relaxing effects of endothelial cells, cytotoxic actions of macrophages and neutrophils, and influences of excitatory amino acids on cerebellar cyclic GMP.
D. Bredt, S. Snyder
semanticscholar +1 more source
Enzymes as chaperones and chaperones as enzymes
Chaperones and foldases are two groups of accessory proteins which assist maturation of nascent peptides into functional proteins in cells. Protein disulfide isomerase, a foldase, and ATPādependent proteases, responsible for degradation of misfolded proteins in cells, both have intrinsic chaperone activities.
Chen Lu Tsou, Chih-chen Wang
openaire +3 more sources
Kinetic Characterization and X-ray Structure of a Mutant of Haloalkane Dehalogenase with Higher Catalytic Activity and Modified Substrate Range [PDF]
Conversion of halogenated aliphatics by haloalkane dehalogenase proceeds via the formation of a covalent alkyl-enzyme intermediate which is subsequently hydrolyzed by water.
Dijkstra, Bauke W.,+7 more
core +3 more sources
Background Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed.
Kristina Volkenborn+5 more
doaj +1 more source
Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle
Background RNA interference (RNAi) has emerged as an efficient tool to control insect pests. When insects ingest double-stranded RNAs (dsRNAs) targeted against essential genes, strong gene silencing and mortality can be induced.
Letian Xu+6 more
doaj +1 more source
Background Laccases are green biocatalysts with wide industrial applications. The study of efficient and specific laccase producers remains a priority.
Long-Bin Zhang+2 more
doaj +1 more source
Conventional serum antibody titer, which expresses antibody level, does not provide antigen binding avidity of the variable region of the antibody, which is essential for the defense response to infection.
Etsuhisa Takahashi+11 more
doaj +1 more source
A new intrinsic thermal parameter for enzymes reveals true temperature optima [PDF]
Two established thermal properties of enzymes are the Arrhenius activation energy and thermal stability. Arising from anomalies found in the variation of enzyme activity with temperature, a comparison has been made of experimental data for the activity ...
Daniel, Roy M.+4 more
core +2 more sources
BACKGROUND Renal function declines progressively in patients who have diabetic nephropathy, and the decline may be slowed by antihypertensive drugs. The purpose of this study was to determine whether captopril has kidney-protecting properties independent
E. Lewis+3 more
semanticscholar +1 more source