Results 41 to 50 of about 3,488,746 (297)

Enzyme Kinetics by Isothermal Titration Calorimetry: Allostery, Inhibition, and Dynamics

Frontiers in Molecular Biosciences, 2020
Isothermal titration calorimetry (ITC) involves accurately measuring the heat that is released or absorbed in real time when one solution is titrated into another. This technique is usually used to measure the thermodynamics of binding reactions. However,
Yun Wang, Guanyu Wang, Nicolas Moitessier, Anthony K. Mittermaier   +3 more
doaj   +1 more source

Cofilin activation in pancreatic acinar cells plays a pivotal convergent role for mediating CCK-stimulated enzyme secretion and growth

Frontiers in Physiology, 2023
Introduction: The actin regulatory protein, cofilin plays a key signaling role in many cells for numerous cellular responses including in proliferation, development, motility, migration, secretion and growth.
Irene Ramos-Alvarez, Lingaku Lee, Lingaku Lee, Robert T. Jensen   +3 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

X-ray structure and enzymatic study of a bacterial NADPH oxidase highlight the activation mechanism of eukaryotic NOX

eLife
NADPH oxidases (NOX) are transmembrane proteins, widely spread in eukaryotes and prokaryotes, that produce reactive oxygen species (ROS). Eukaryotes use the ROS products for innate immune defense and signaling in critical (patho)physiological processes ...
Isabelle Petit-Hartlein, Annelise Vermot, Michel Thepaut, Anne-Sophie Humm, Florine Dupeux, Jerome Dupuy, Vincent Chaptal, Jose Antonio Marquez, Susan ME Smith, Franck Fieschi   +9 more
doaj   +1 more source

Activation of the Bradykinin System by Angiotensin-Converting Enzyme Inhibitors

European Journal of Inflammation, 2010
The Bradykinin (BK) system has a very significant role in the regulation of blood pressure (BP). Hence, reduced activity of BK receptors mediated via decreased circulating endogenous kinin might explain the cause of high BP.
J.N. Sharma
doaj   +1 more source

Subcellular localization of hexadecanedioic acid activation in human liver

Journal of Lipid Research, 1974
The activation of hexadecanedioic acid has been studied in subcellular fractions of human liver. The activation capacity in a total homogenate of human liver was found to be 0.5 μmole/min/g wet wt of tissue, about 10% of that for palmitic acid ...
Jon Elling Pettersen, Magne Aas
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

β-Galactosidase- and Photo-Activatable Fluorescent Probes for Protein Labeling and Super-Resolution STED Microscopy in Living Cells

Molecules
We report on the synthesis of two fluorescent probes which can be activated by β-Galactosidase (β-Gal) enzymes and/or light. The probes contained 2-nitro-4-oxybenzyl and 3-nitro-4-oxybenzyl fragments, with β-Gal residues linked to C-4.
Taukeer A. Khan, Stefan Stoldt, Mariano L. Bossi, Vladimir N. Belov, Stefan W. Hell   +4 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Structural characteristics of the nonallosteric human cytosolic malic enzyme

, 2017
Human cytosolic NADP(+)-dependent malic enzyme (c-NADP-ME) is neither a cooperative nor an allosteric enzyme, whereas mitochondrial NAD(P)(+)-dependent malic enzyme (m-NAD(P)-ME) is allosterically activated by fumarate.
Hsieh, Ju-Yi;Li, Shao-Yu;Chen, Meng-Chun;Yang, Pai-Chun;Chen, Hui-Yi;Chan, Nei-Li;Liu, Jyung-Hurng;Hung, Hui-Chih, 詹迺立   +1 more
core   +1 more source