Results 41 to 50 of about 1,754,066 (244)

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Extremophile enzyme activity lab: using catalase from Pyrobaculum calidifontis to highlight temperature sensitivity and thermostable enzyme activity

Journal of Microbiology & Biology Education
There are places on earth that are considered to possess extreme physico-chemical characteristics as they relate to life. Surprisingly, there are microbes that have adapted various strategies that enable them to form robust communities in these ...
Joseph W. Scott, J. Jordan Steel
doaj   +1 more source

Dietary cholesterol increases paraoxonase 1 enzyme activity

Journal of Lipid Research, 2012
HDL-associated paraoxonase 1 (PON1) activity has been consistently associated with cardiovascular and other diseases. Vitamins C and E intake have previously been positively associated with PON1 in a subset of the Carotid Lesion Epidemiology and Risk ...
Daniel S. Kim, Amber A. Burt, Jane E. Ranchalis, Rebecca J. Richter, Julieann K. Marshall, Karen S. Nakayama, Ella R. Jarvik, Jason F. Eintracht, Elisabeth A. Rosenthal, Clement E. Furlong, Gail P. Jarvik   +10 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

ANTIOXIDANT ENZYME ACTIVITY AND FRESH-CUT ARRACACHA QUALITY

Ciência e Agrotecnologia, 2015
The arracacha is an alternative of fresh-cut product; however it can be easily degraded after the processing techniques. The objective of this work was to evaluate the useful life of fresh-cut arracacha submitted to two types of cuts and storage, as well
Hêmina Carla Vilela, Patrícia de Fátima Pereira Goulart, Kamila Rezende Dázio de Souza, Ana Carolina Vilas Boas, Jane Silva Roda, Roseane Maria Evangelista de Oliveira   +5 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

A Review of Enzyme Immobilization Methods and Critical Parameters for Evaluating Immobilized Enzymes [PDF]

Journal of Mazandaran University of Medical Sciences
‌Enzymes, natural biocatalysts with high catalytic efficiency, are essential in numerous biological and industrial processes. However, their inherent instability and sensitivity to environmental conditions often limit their direct application.
Mehdi Aghaee, Mehdi Mogharabi-Manzari
doaj  

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

SOME PROPERTIES AND FACTORS AFFECTING CHITINASE PRODUCTION BY Bacillus licheniformis AND ITS USE AS ANTIFUNGAL AGENT [PDF]

Journal of Food and Dairy Sciences, 2000
  Chitinolytic enzymes of B. licheniformis , which used as antifungal agent were studied, and the results revealed that the growth of B. licheniformis reached its maximum activity in the third day.  Maximum enzyme activity was observed in the fifth day. 
M. Demerdash, M. El-Sawah, T. Shady,
doaj   +1 more source